Chinese wolfberry fermented raw stock cosmetic and preparation method thereof
A technology of fermented puree and cosmetics, which is applied in the field of wolfberry fermented puree cosmetics and its preparation, can solve the problems that wolfberry has not been developed, and achieve the effects of maintaining natural activity, ensuring safety, and avoiding the loss of active ingredients
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Embodiment 1
[0034] Embodiment 1, preparation and detection of wolfberry fermented protoplasm cosmetics
[0035] The concentration in the expansion medium was 10 6 -10 8 Inoculate 20ml of CFU / ml Lactobacillus delbrueckii liquid into 15g of 50 mesh wolfberry fruit powder and 200g of water, and ferment in a 45°C incubator for 16 hours. Inactivation, the sterilized fermented liquid is 12000r / min, the centrifugal radius is 9cm; the centrifugal time is 20min, and the supernatant is collected, which is the fermented wolfberry puree cosmetic provided by the present invention.
[0036] The appearance of the wolfberry fermented puree cosmetic prepared in this example is viscous liquid, and the color is light red to deep red. The pH value is 5.2-6.8, the viscosity is 50-300cP, the mass percentage of soluble solids is 1.5-5.0%, the total number of colonies is less than 50CFU / ml, and no pathogenic bacteria is detected. It meets the quality requirements of cosmetics.
[0037] The component analysis...
Embodiment 2
[0039] Example 2, Antioxidative Efficacy Analysis of Lycium Barbarum Fermented Puree Cosmetics
[0040] DPPH is an early synthesized organic free radical, which is often used to evaluate the hydrogen-donating ability of antioxidants. It is very stable in organic solvents, has a purple color, and has a characteristic absorption peak at , when it encounters a free radical scavenger , the lone pair of electrons of DPPH is paired to make it fade, that is, the light absorption value at the maximum absorption wavelength becomes smaller. Therefore, the scavenging effect of the sample on DPPH free radicals can be evaluated by measuring the change of the absorbance value.
[0041] The specific experimental steps of the DPPH free radical scavenging experiment are as follows:
[0042] (1) Take an equal volume (usually 3mL) of the solution to be tested and 2×10 -4 mol / L DPPH solution mixed (A 1 Tube);
[0043] (2) Take an equal volume of absolute ethanol (the solvent for the analyte) ...
Embodiment 3
[0049] Example 3, safety evaluation of wolfberry fermented protoplasm
[0050] The patch test is mainly used to detect the irritation of cosmetic final products or raw materials. In the invention, the human body closed patch test is performed on the wolfberry fermented puree cosmetic to evaluate its potential skin irritation.
[0051] Choose 30 suitable volunteers, randomly selected in the age range of 18-60 years old. Weigh 0.020g-0.025g and put the solid sample or semi-solid sample into the spot tester for later use. Add 0.2mL to 0.025mL of the liquid sample dropwise on the filter paper, and then place the filter paper in the spot tester. Set a blank control for each sample, and add the same amount of sample solvent as the sample, such as distilled water or olive oil, into the hole of the control spot tester.
[0052] The test site is selected as the back of the human body, and the spot tester is fixed and pasted on the back of the subject with a non-irritating adhesive t...
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Abstract
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Application Information
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