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Application of osgsl5 protein in controlling plant fertility

A plant and fertility technology, applied in the field of OsGSL5 protein in the control of plant fertility, can solve the problems of microspore development process interference, few reports on the molecular regulation mechanism of callose degradation, and abnormal deposition of the outer wall

Inactive Publication Date: 2017-05-17
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can be inferred that the early or delayed degradation of the callus wall will interfere with the development of microspores, and lead to abnormal deposition of the outer wall, resulting in plant sterility
However, there are few reports on the molecular regulation mechanism of callose degradation during pollen development.

Method used

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  • Application of osgsl5 protein in controlling plant fertility
  • Application of osgsl5 protein in controlling plant fertility
  • Application of osgsl5 protein in controlling plant fertility

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1, OsGSL5 gene mutant gsl5-1 acquisition and phenotype

[0041] 1. Obtaining of gsl5-1 mutant

[0042] 1. Obtaining the gsl5-1 mutant

[0043] The rice T-DNA insertion mutant library used was constructed by the Army Biotechnology Research Institute of Chinese Agricultural Sciences using the vector pFX-E24.2-15R (the method for creating the mutant library has been published in a paper, see Wan et al., Activation tagging, an efficient tool for functional analysis of the rice genome. Plant Mol Biol. 2009 Jan; 69(1-2):69-80).

[0044] 10,000 seeds of the transgenic rice variety "Nihonbare (a sequenced japonica variety maintained by the Biotechnology of the Chinese Academy of Agricultural Sciences)" were selected, soaked and accelerated germination, sowed in the seedling field, and transplanted to the field 35 days later. Two rows of each material, 10 plants per row, constitute a family. The planting location is the experimental field in the high-tech industrial pa...

Embodiment 2

[0056] Embodiment 2, the application of the substance that suppresses the expression of OsGSL5 in cultivating rice sterile material

[0057] 1. Preparation of DNA molecules

[0058] Primers were designed according to the CDS sequence (sequence 1) of the OsGSL5 gene as follows: 5'-GCT TAT CTG AAA TCA TCTTGT CTC TC-3' and 5'-CCA AGA TGC GGG AGA TCT G-3'.

[0059] Total RNA was extracted from rice Nipponbare seedlings, and cDNA was synthesized from RNA using reverse transcriptase. Using the cDNA obtained by reverse transcription as a template, PCR amplification was performed with the above primers, and the PCR product was detected by 0.8% agarose gel electrophoresis, and a band with a molecular weight of about 540 bp was obtained. This fragment was recovered using an agarose gel recovery kit (TIANGEN). The recovery fragment was connected with pMD19-TSimple (Takara), the connection product was transformed into Escherichia coli DH5α competent cells, positive clones were screened ...

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Abstract

The invention discloses an application of an OsGSL5 protein in controlling plant fertility. The invention provides the application of a substance for inhibiting the expression of an OsGSL5 encoding gene in regulating and controlling the plant fertility. The amino acid sequence of the OsGSL5 protein is the sequence 2 in the sequence table. Experiments prove that sterile rice can be obtained by silencing the OsGSL5 gene expression in the rice by use of an antisense nucleic acid, and thus prove that the OsGSL5 gene is capable of regulating and controlling the fertility of the rice; the role that the OsGSL5 gene plays in the pollen development and formation process has an important application prospect especially in creating manually controllable male sterility, creating hybrid rice and creating an acceptor material of genetically modified organism biosafety.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of OsGSL5 protein in controlling plant fertility. Background technique [0002] Rice is one of the most important food crops in the world. It feeds half of the world's population. How to increase rice production is an important issue related to the national economy and people's livelihood. At present, the vast majority of rice in my country is produced by hybrid rice, and breeding sterile lines by controlling pollen fertility is the key to hybrid rice production. Some nuclear genes or cytoplasmic male sterile lines used in the production of hybrid rice play an important role in rice hybrid breeding and yield increase. [0003] Rice pollen development is a very complex cellular process, involving all stages of pollen development, such as meiosis, mitosis, tapetum cell development, anther wall formation, etc. (Liu et al.2005). An abnormality in any process or link may...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/82C12N15/29A01H5/00
CPCC07K14/415C12N15/8289
Inventor 吴金霞张治国孙学辉路铁刚
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI