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Method for producing organic fertilizer through biodegradation of died livestock

A technology of dead livestock and poultry and organic fertilizer, applied in the field of microorganisms, can solve problems such as elimination and increase of sick and dead livestock, ecological environment pollution, destruction, etc., to achieve the effect of ensuring healthy and sustainable development, prolonging the release cycle, and reducing huge pressure

Inactive Publication Date: 2015-01-28
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The prevalence and outbreak of various animal diseases caused by the large-scale modern animal husbandry production mode has caused a sharp increase in the number of eliminated and dead livestock and poultry; moreover, due to social awareness, technology and cost of the harmless treatment of eliminated and dead livestock and poultry, etc. , so that incidents of littering and burying sick and dead livestock and poultry continue to occur, which not only creates opportunities for the spread of animal diseases, but also causes great pollution and damage to the ecological environment

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1

[0031]Example 1-1: Cultivation method of Bacillus subtilis-1 (ZJNB0001)

[0032] Inoculate the purified culture of Bacillus subtilis-1 (ZJNB0001) into a shaker flask with a volume content of 15% of the activation medium-1. Cultivate for 20 hours at 37°C with a shaker flask rotation speed of 180rpm; inoculate the activated Bacillus subtilis strain into a bioreactor with a volume content of 50% of medium-1, and the volume inoculum of the strain is 1% , cultured for 20 hours at a culture temperature of 37°C, a ventilation rate of 8 L / s, and a bioreactor stirring speed of 220 rpm, and the content of Bacillus subtilis in the tank was ≥ 10 9 a / ml;

[0033] The formula of described activation medium-1 is glucose 18g / l, peptone 12g / l, sodium chloride 3g / l and beef extract 0.2g / l;

[0034] The formula of the medium-1 is corn flour 10g / l, glucose 3g / l, bean cake powder 18g / l, fish meal 3g / l, calcium carbonate 5g / l, ammonium sulfate 0.8g / l, dipotassium hydrogen phosphate 0.1g / l, magn...

Embodiment 1-2

[0035] Example 1-2: Cultivation of Bacillus subtilis-2 (ZJHZ0005)

[0036] Inoculate the purified cultured Bacillus subtilis-2 (ZJHZ0005) into a shaker flask with a volume content of 15% of the activation medium-1. Cultivate for 20 hours at 37°C with a shaker flask rotation speed of 180rpm; inoculate the activated Bacillus subtilis strain into a bioreactor with a volume content of 50% of medium-1, and the volume inoculum of the strain is 1% , cultured for 20 hours at a culture temperature of 37°C, a ventilation rate of 8 L / s, and a bioreactor stirring speed of 220 rpm, and the content of Bacillus subtilis in the tank was ≥ 10 9 a / ml;

[0037] The formula of described activation medium-1 is glucose 18g / l, peptone 12g / l, sodium chloride 3g / l and beef extract 0.2g / l;

[0038] The formula of the medium-1 is corn flour 10g / l, glucose 3g / l, bean cake powder 18g / l, fish meal 3g / l, calcium carbonate 5g / l, ammonium sulfate 0.8g / l, dipotassium hydrogen phosphate 0.1g / l, magnesium su...

Embodiment 1-3

[0039] Embodiment 1-3: the culture method of Bacillus licheniformis (ZJHZ 1025)

[0040] Inoculate the purified and cultured Bacillus licheniformis (ZJHZ 1025) into a shaker flask with a volume content of 15% of the activation medium-2. , Shake flask rotating speed is to cultivate 20h under 180rpm; The bacillus licheniformis bacterial classification that the activation culture is good is inoculated in the bioreactor that is equipped with volumetric content and is 50% fermentation medium, and the volume inoculum of bacterial classification is 1%, in cultivation The temperature is 37°C, the ventilation rate is 8L / s, and the stirring speed of the bioreactor is 200rpm, cultivated for 20h, and the content of Bacillus licheniformis in the tank is ≥ 10 9 a / ml;

[0041] The formula of described activation medium-2 is peptone 8g / l, beef extract 1g / l and sodium chloride 3g / l;

[0042] The formula of the fermentation medium is 13g / l of bean cake powder, 13g / l of corn flour, 0.05g / l of ...

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Abstract

The invention discloses a method for producing an organic fertilizer through biodegradation of died livestock. The method comprises the steps of fully mixing died livestock bodies with a compound microbial agent according to a proportion of 100Kg: (30-45L), then fully mixing with chicken manure, sludge and straw, putting into a fermentation tank for fermenting, and turning over in fermentation, wherein the ventilator capacity is 0.8-1.0VVM, the pressure of the tank is kept at 0.03-0.05Mpa, the temperature is at 55-62DEG C, and the period is 2-3 days. The organic fertilizer can reduce the cost of the fertilizer by over 65%, and can prolong the fertilizer nutrient release period to about 120%; due to adoption of the organic fertilizer, great pressure of the livestock died of disease on the environment can be lowered, so that the habitat balance is maintained; the compound microbial agent can ferment and degrade the livestock died of disease into organic fertilizer safely and efficiently in short time, so that the great pressure of the livestock died of disease on the environment can be lowered, furthermore, the waste is turned into wealth.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a method for preparing organic fertilizer by biodegrading sick and dead livestock and poultry, and a preparation method and application of a compound microbial bacterial agent. Background technique [0002] The prevalence and outbreak of various animal diseases caused by the large-scale modern animal husbandry production mode has caused a sharp increase in the number of eliminated and dead livestock and poultry; moreover, due to social awareness, technology and cost of the harmless treatment of eliminated and dead livestock and poultry, etc. , so that the incidents of littering and burying sick and dead livestock and poultry continue to occur everywhere, which not only creates opportunities for the spread of animal diseases, but also causes great pollution and damage to the ecological environment. If the biodegradation of dead livestock and poultry can be used, it can not only saf...

Claims

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Application Information

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IPC IPC(8): C05F11/08
CPCC05F1/00C05F17/00C05F3/00C05F7/00C05F11/00C05F11/08Y02A40/70
Inventor 韩剑众曲道峰吴劲松余全法竺礼斌
Owner ZHEJIANG GONGSHANG UNIVERSITY
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