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A canine stem cell-secreted factor repair solution for rapid healing of canine traumatic tissue

A technology for secreting factors and stem cells, which can be used in drug combinations, organic active ingredients, and inactive medical preparations, etc. It can solve the problems of expensive tissue-engineered skin, long production cycle, and complex preparation technology, and achieve good scientific research and development. Clinical value, high active effect

Active Publication Date: 2018-09-11
严玉霖
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems are poor anti-infection, delayed vascularization, complicated preparation technology, long production cycle, high price, and physiological defects of tissue-engineered skin.

Method used

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  • A canine stem cell-secreted factor repair solution for rapid healing of canine traumatic tissue

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Effect test

Embodiment 1

[0015] Embodiment 1: A repairing solution for fast healing of dog wound tissue, the repairing solution includes canine stem cell active ingredient extract, hyaluronic acid and preservative calcium propionate.

[0016] The specific extraction steps of the repair solution are as follows:

[0017] Step 1, in vitro isolation of canine BMSCs: Routinely anesthetize the puppies, prepare the skin of the posterior superior iliac spine, sterilize, spread a drape, extract about 10ml of bone marrow at 2-3 points with a bone marrow puncture needle containing 1ml of heparin sodium, mix well to prevent coagulation , quickly transferred to a 50ml sterilized centrifuge tube, diluted and mixed with an equal amount of DMEM / F12; take 10 centrifuge tubes with 5ml of lymphocyte separation solution added to the bottom, the concentration of the added lymphocyte separation solution was 1.077g / ml, and the upper layers were respectively attached to the wall Flow in an equal amount of bone marrow diluent...

Embodiment 2

[0031] Embodiment 2: The repair solution prepared in Embodiment 1 is applied.

[0032] Treat dogs of different breeds, ages, types and degrees of soft tissue open wounds received clinically by Kunming Emerson Pet Hospital, including bite wounds and laceration wounds caused by mutual biting and fighting. Cutting or cutting wounds caused by chopping objects, contusions caused by blunt objects, pressure wounds caused by rolling wheels or heavy objects, and skin, eye and corneal ulcers, etc., the clinical symptoms before treatment are hemorrhage, Pain, wound infection causes suppuration, and some manifest as inflammatory reactions such as elevated body temperature and increased white blood cell count.

[0033] Anti-shock treatment, analgesia, rehydration, and debridement are carried out for severe wounds first, and then the repair solution of the present invention is used for treatment; for dogs with systemic inflammatory reactions, antibiotics are first used for treatment, and th...

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Abstract

A dog stem cell excreted factor repair liquid capable of quickly healing dog wound tissue relates to the technical field of biology and especially to the technical field of dog stem cells. The repair liquid comprises a dog stem cell active component extract which is extracted from dog bone marrow substrate stem cells and includes various active growth factors, collagen, active polypeptide, micro elements, hyaluronic acid and the like. The repair liquid can quickly promote growth, activation and regeneration of cells and tissue and can achieve a net-typed adjustment effect on treatment of dog refractory wounds. The dog stem cell excreted factor repair liquid can quickly and effectively promote a wound on skin and a surgical wound to heal, can repair ulceration on skin and eyes and has a good scientific and clinical value.

Description

technical field [0001] The invention relates to canine stem cell technology in the field of biotechnology, in particular to a preparation method and clinical application of canine stem cell secreted factor repair solution for rapidly promoting canine wound tissue healing. Background technique [0002] Canine bone marrow stromal cells (BMSC) is a cell population with self-renewal and multilineage differentiation potential in bone marrow. With the characteristics of wide source, convenient collection, easy expansion in vitro, and low immunogenicity, it is an ideal seed source cell for regenerative medicine, tissue engineering, and gene therapy. Canine BMSCs can differentiate in a specific environment, so as to repair cell and tissue damage. Studies have shown that in addition to the differentiation of stem cells themselves, their paracrine effects play a greater role in the repair process. Canine BMSCs can secrete a large number of cytokines, and the secreted factors can be ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/28A61P17/02
CPCA61K9/0014A61K31/728A61K35/28A61K47/12A61K2300/00
Inventor 严玉霖曹景锋陈玲
Owner 严玉霖