Leukocyte-increasing traditional Chinese medicine decoction piece combined preparation as well as preparation method and combined package thereof
A technology of traditional Chinese medicine decoction pieces and white prescriptions, which can be used in drug combinations, pharmaceutical formulations, medical preparations containing active ingredients, etc.
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[0057] The present invention is further illustrated below by means of examples, but the present invention is not limited to the scope of the examples.
[0058] Table 1 shows the raw materials and their contents of the combined preparations of Shengbai Fang traditional Chinese medicine decoction pieces of Examples 1-4, Comparative Examples 1-4 and Basic Examples.
[0059] The raw material of table 1 embodiment, comparative example and basic example and content thereof
[0060]
[0061] The preparation method of the Shengbaifang Chinese herbal medicine combination preparation of embodiment 1-4 and the product of comparative example 1-4 is:
[0062] Step 1: Grind epimedium, psoraleae, aconite, medlar, astragalus, atractylodes macrocephala and rehmannia glutinosa into 60-80 mesh fine powders, mix well, and soak in 80-90% ethanol that is 6-8 times the raw material 0.5 to 2 hours, heated and refluxed for 1 to 3 hours, extracted 1 to 3 times, filtered to remove medicinal residues...
Embodiment 5
[0070] The present invention also provides a combined package of a combined preparation of Shengbaifang traditional Chinese medicine decoction pieces, including Epimedium, Psoraleae, Aconite, Lycium barbarum, Astragalus, Caulis Spatholobus, Rubia, Angelica, Reed Rhizome, Ophiopogon japonicus, Licorice, Atractylodes macrocephala .
[0071] The package also includes auxiliary materials, including epimedium, psoraleae, aconite, medlar, astragalus, spatholobus, madder, angelica, reed rhizome, radix radix radix radices, licorice, Atractylodes macrocephala, rehmannia glutinosa, ginseng, scrophulariaceae, The ratio of the total amount of Tongcao to the auxiliary material is 1:1, and the auxiliary material is hydroxypropyl-β-cyclodextrin.
Embodiment 6
[0073] White blood cell phagocytosis test
[0074] Experimental principle: mix the white blood cells to be tested with staphylococci, and after incubating at 37°C for a certain period of time, the bacteria can be phagocytized by neutrophils. The number of leukocytes in phagocytes and the total number of phagocytized bacteria were calculated to calculate the phagocytosis rate and phagocytosis index, reflecting the phagocytosis function of neutrophils.
[0075] Materials: The equipment includes inoculation loops, small test tubes, micro-cell culture plates, water baths, glass slides, microscopes, etc. Preparation of bacteria solution: Take the lawn of Staphylococcus albus cultured on agar slant or plate for 24 hours, wash it twice with PBS (0.01mol / L pH6.5), and sterilize it in a boiling water bath for 15-20 minutes. Suspend the inactivated bacteria solution in 20% culture medium, adjust the cell concentration to 5*1000 / L by turbidimetry, and set aside at 4°C. 100U / ml heparin / ...
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