A kind of recombinant trail protein and its preparation method and application

A proteasome inhibitor and protein technology, which is applied in the field of recombinant TRAIL protein and its preparation and application, can solve problems such as instability and short half-life, and achieve strong stability, long half-life in vivo, and obvious effects
CN104342444BActive Publication Date: 2017-11-17WEST CHINA HOSPITAL SICHUAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
WEST CHINA HOSPITAL SICHUAN UNIV
Publication Date
2017-11-17

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Abstract

Provided is a nucleic acid fragment, the nucleotide sequence thereof being shown in SEQ ID NO: 1. Provided is a recombinant protein encoded by said nucleic acid fragment, and a preparation method and use of said recombinant protein. Also provided are a conjugate of a recombinant protein modified by PEG, and a preparation method and use of said recombinant protein. The recombinant protein has anti-tumor activity, and acts in synergy with a proteasome inhibitor; the PEG modification of the recombinant protein, while retaining anti-tumor drug effects, also has improved stability and a longer half-life.
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Description

technical field

[0001] The invention relates to a recombinant TRAIL protein and its preparation method and application. Background technique

[0002] Tumor necrosis factor-related apoptosis-inducing ligand (TNF related apoptosis-inducing ligand, TRAIL), also known as apoptin 2 ligand, is a member of the TNF family discovered in recent years that can induce tumor cell apoptosis. The combination of receptors exerts a selective killing effect on tumor cells. There are four types of TRAIL membrane receptors, including DR4, DR5, DcR1, and DcR2. Among them, DR4 and DR5 are death receptors (death receptors), which are highly expressed in tumor cells, and their molecules contain a death domain. When TRAIL binds to it After that, it will transmit signals through its death domain to induce apoptosis; DcR1 and DcR2 are decoy receptors (decoy receptors), highly expressed in normal cells, which also have the ability to bind TRAIL, but do not contain a death domain in the molecule , TRA...

Claims

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