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Cationic antibacterial peptide as well as preparation method and application thereof

An antibacterial peptide and cation technology, applied in the field of biopharmaceuticals, can solve the problems of low antibacterial activity, high hemolytic toxicity, complex structure, etc., and achieve the effects of good antibacterial effect, broad-spectrum killing activity and simple structure

Inactive Publication Date: 2015-02-18
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the currently disclosed cationic antimicrobial peptides have complex structures, low antibacterial activity and high hemolytic toxicity. Therefore, it is urgent to develop cationic antimicrobial peptides with simple structure, high antibacterial activity, easy chemical synthesis and low hemolytic toxicity.

Method used

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  • Cationic antibacterial peptide as well as preparation method and application thereof
  • Cationic antibacterial peptide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Preparation of antimicrobial peptides (take the preparation of 0.1 mmol as an example):

[0030] The preparation is carried out one by one from the N-terminal to the C-terminal, and is automatically controlled by the pioneer peptide synthesizer. First weigh 0.1 mmol of the resin combined with the first amino acid, Phe (purchased from Applied Biosystems, USA), pack it into a column, and then use 20% (v / v) piperidine dimethylformamide solution for deprotection, and then Washed with dimethylformamide, the amino acid Arg protected with 9-Fat methoxycarbonyl (Fmoc) was dissolved in carbodiimide (DCC), and hydroxybenzotriazole (HOBt) / diisopropylethyl Amine (DIPEA), the dissolved solution was circulated on the column for coupling reaction for 30 minutes, washed with trifluoroacetic acid (TFA), and the above deprotection to coupling reaction steps were repeated until all amino acids in the SK1 sequence were inserted, and the preparation was completed. (For the specific ope...

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PUM

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Abstract

The invention discloses cationic antibacterial peptide as well as a preparation method and application thereof. The sequence of the cationic antibacterial peptide is as follows: SK1:FRWWR-NH2((Phe-Arg-Trp-Trp-Arg-NH2); the preparation method is a solid phase chemical synthesis method. the cationic antibacterial peptide provided by the invention has broad-spectrum killing activity to gram-positive bacteria and gram-negative bacteria and has higher bactericidal activity when being compared with natural antibacterial peptide; besides, the cationic antibacterial peptide has the advantages that the structure is simple, artificial synthesis is convenient, no decorative connection is required, the hemolytic toxicity is small, and no toxic effect is caused to animal and plant cells; the cationic antibacterial peptide has very important values in the aspects of development and application of antibacterial medicines.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and relates to an antibacterial peptide and its preparation method and application, in particular to a cationic antibacterial peptide, its preparation method and its application in treating bacterial infection diseases. [0002] Background technique [0003] Since the invention of antibiotics, humans have made great achievements in controlling and treating microbial infections. However, with the continuous use of antibiotics, microbial resistance has become a major problem in microbial infection control, so that some microbial bacteria have no control to kill substances, such as in clinical drug treatment, vancomycin-resistant grapes coccus, enterococci, and other Gram-negative bacterial infections, and the treatment of these diseases is a worldwide problem. [0004] Antimicrobial peptides are a class of small molecular polypeptides widely present in microorganisms, animals and plant...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/20C07K1/06C07K1/04A61K38/08A61P31/04
Inventor 王远强周朋朋夏庆友赵萍林治华丁元钟晓武
Owner SOUTHWEST UNIVERSITY
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