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Biological processing method for probiotics cottonseed polypeptide

A technology for bioprocessing and cottonseed is applied in the field of bioprocessing of probiotic cottonseed polypeptides to achieve the effects of inhibiting pathogen infection, high digestion and absorption rate, and easy digestion and absorption

Inactive Publication Date: 2015-03-04
源耀生物科技(盐城)股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a bioprocessing method of probiotic cottonseed polypeptide, which mainly solves the problems of improving the digestion and absorption rate of cottonseed protein, improving the yield of small peptides and the degree of protein hydrolysis, improving the palatability of cottonseed protein, and being used in feed to improve animal resistance. sick problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Cottonseed protein powder 100kg, sterilized at 110°C for 10 minutes, add 20kg of Bacillus subtilis seed liquid, 0.2kg of alkaline protease, 0.1kg of flavor protease and 70kg of tap water to prepare the inoculation mixture, mix well, adjust the pH value to 7.2, put in Temperature-controlled fermentation in the fermentation tank, the thickness of the material layer is 20cm, the bottom is placed in a ventilated environment, fermented at 30°C for 60 hours, dried at 60°C, crushed and packaged to obtain the finished product.

[0041] The product is yellow in color with aroma, 53.3% crude protein, 26.9% small peptide content, 3*10^8 live bacteria / g, and 51% increase in antioxidant capacity.

Embodiment 2

[0043]Cottonseed protein powder 100kg, sterilized at 120°C for 5 minutes, add 15kg of Bacillus subtilis seed solution, 0.2kg of alkaline protease, 0.2kg of flavored protease and 65kg of tap water to prepare the inoculation mixture, mix well, adjust the pH value to 7.2, put in Temperature-controlled fermentation in the fermentation tank, the thickness of the material layer is 15cm, placed in a ventilated environment, fermented at 37°C for 72 hours, dried at 70°C, crushed and packaged to obtain the finished product.

[0044] The product is yellow in color with aroma, 51.2% crude protein, 26.9% small peptide content, 1*10^8 live bacteria / g, and 63% increase in antioxidant capacity.

Embodiment 3

[0046] Cottonseed protein powder 100kg, sterilized at 105°C for 15 minutes, add 15kg of Bacillus subtilis seed solution, 0.2kg of alkaline protease, 0.1kg of flavored protease and 75kg of tap water to prepare the inoculation mixture, mix well, adjust the pH value to 7.2, put in Temperature-controlled fermentation in the fermentation tank, the thickness of the material layer is 30cm, placed in a ventilated environment, fermented at 43°C for 72 hours, dried at 75°C, crushed and packaged to obtain the finished product.

[0047] The product is yellow in color with aroma, 52.9% crude protein, 24.8% small peptide content, 6*10^7 live bacteria / g, and 55% increase in antioxidant capacity.

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Abstract

The invention discloses a biological processing method for probiotics cottonseed polypeptide. According to the method, an excellent-quality cottonseed protein product is obtained by utilizing bacillus subtilis and an enzyme preparation to process cottonseed protein and fermenting at 30 DEG C for 2-3 d. The cottonseed protein product has the free gossypol content lower than the safe standard prescribed by the forage, the crude protein content of 50%, the small peptide content of 25%, the oxidation resistance improved by 53% and substantially-increased free amino acid content, and can be directly used as a protein forage. The biological processing method relatively well solves the problems that a product produced by employing a prior art is bad in sensory quality and bad in palatability and the protein quality becomes bad, is a cottonseed protein biological processing method which is capable of substantially improving nutrition value and oxidation resistance, is low in energy consumption and also enables the cottonseed protein to reach the forage standards, and has wide application prospect.

Description

technical field [0001] The invention relates to a bioprocessing method for co-processing cottonseed protein powder by using microbial preparations and enzyme preparations, in particular to a bioprocessing method for probiotic cottonseed polypeptides. Background technique [0002] my country is a big cotton-producing country, and its cotton output ranks first in the world. The annual output of cottonseed meal that can be used as protein resources is nearly 9 million tons, accounting for 10.7% of the total protein meal. At present, cottonseed protein products mainly include cottonseed meal and cottonseed protein powder. Cottonseed meal contains more than 40% high-quality protein resources. However, this huge protein resource limits its use in feed because of the toxin-gossypol. heavily utilized. [0003] Cottonseed protein powder is a newly developed protein product. Its protein content is as high as 50%. At the same time, its gossypol content is far lower than cottonseed mea...

Claims

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Application Information

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IPC IPC(8): A23K1/00A23K1/14
Inventor 孙中超朱校适方华夏佳吉俞涵丽季春源
Owner 源耀生物科技(盐城)股份有限公司
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