Method for extracting purified phytosterol from corn stigma

A technology of phytosterols and corn silk, which is applied in the field of extraction and purification of natural phytosterols, can solve the problems of uneconomical economic benefits, complex extraction process, and low product recovery rate, and achieve simple extraction equipment, short process flow, and high product purity. Effect

Active Publication Date: 2015-03-11
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Traditionally, phytosterols are derived from vegetable oil residues and tall oil pitch obtained from the papermaking process. However, these extraction methods have defects such as high extraction temperature, complicated extraction process, long process, large solvent consumption, and low product recovery rate. The sterols produced in the world are far from meeting the needs of industrial production
Chinese patent CN102250198A discloses a method for extracting phytosterols from dragon fruit stems using supercritical fluid extraction technology, but this method consumes a lot of fluid and has high costs, which is very uneconomical from the perspective of economic benefits

Method used

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  • Method for extracting purified phytosterol from corn stigma
  • Method for extracting purified phytosterol from corn stigma
  • Method for extracting purified phytosterol from corn stigma

Examples

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Effect test

Embodiment 1

[0024] A method for extracting and purifying phytosterols from corn silk, which comprises the following steps:

[0025] 1) Grind the removed corn silk with a grinder, pass through a 60-mesh sieve, and then dry it in an oven (80°C). Take 50g of crushed and dried corn silk and saponify with 100ml of saponification solution at 85°C for 3h, then add 250ml of absolute ethanol for ultrasonic extraction for 0.5h, centrifuge for 30min after ultrasonic extraction, take the supernatant, and rotary evaporate to obtain the crude extract. Described saponification liquid is the potassium hydroxide ethanol solution of concentration 3mol / L, is about to dissolve an appropriate amount of potassium hydroxide in 80V% ethanol to obtain saponification liquid;

[0026] 2) The crude extract was extracted with a mixed solution of water and ether at a volume ratio of 1:5, and the organic phase was rotary evaporated to obtain a concentrated extract, which was then recrystallized with ethyl acetate to ob...

Embodiment 2

[0028] A method for extracting and purifying phytosterols from corn silk, which comprises the following steps:

[0029] 1) Grind the removed corn silk with a grinder, pass through a 60-mesh sieve, and then dry it in an oven (80°C). Take 50g of crushed and dried corn silk and saponify with 300ml of saponification solution at 75°C for 4h, then add 300ml of absolute ethanol for ultrasonic extraction for 1h, centrifuge for 30min after ultrasonic extraction, take the supernatant, and rotary evaporate to obtain the crude extract. Described saponification liquid is the potassium hydroxide ethanol solution of concentration 4mol / L, is about to dissolve appropriate amount of potassium hydroxide in 90V% ethanol to obtain saponification liquid;

[0030] 2) The crude extract was extracted with a mixed solution of water and ether at a volume ratio of 1:6, and the organic phase was rotary evaporated to obtain a concentrated extract, which was then recrystallized from ethyl acetate to obtain ...

Embodiment 3

[0032] A method for extracting and purifying phytosterols from corn silk, which comprises the following steps:

[0033] 1) Grind the removed corn silk with a grinder, pass through a 60-mesh sieve, and then dry it in an oven (80°C). Take 50g of crushed and dried corn silk and saponify with 200ml of saponification solution at 90°C for 2h, then add 400ml of absolute ethanol for ultrasonic extraction for 0.5h, centrifuge for 30min after ultrasonic extraction, take the supernatant, and rotary evaporate to obtain the crude extract. Described saponification liquid is the potassium hydroxide ethanol solution of concentration 3mol / L, is about to be dissolved in an appropriate amount of potassium hydroxide in 85V% ethanol to obtain saponification liquid;

[0034] 2) The crude extract was extracted with a mixed solution of water and ether at a volume ratio of 1:8, and the organic phase was rotary evaporated to obtain a concentrated extract, which was then recrystallized with ethyl acetat...

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Abstract

The invention relates to a method for extracting purified phytosterol from corn stigma. The method comprises the following steps: 1) adding 1g of crushed and dried corn stigma into 2-6 ml of a saponifying solution, saponifying for 2-4 hours at 75-95 DEG C, adding absolute ethyl alcohol, performing ultrasonic extraction for 0.5-1 hour, centrifuging after ultrasonic extraction, taking a supernatant, and performing rotary evaporation on the supernatant to obtain a crude extract, wherein the saponifying solution is a potassium hydroxide ethanol solution with the concentration of 2-5 mol / L; 2) extracting the crude extract with a solvent, and recrystallizing by using ethyl acetat to obtain a finished product. The method is easy to operate, simple and short in process flow and low in production cost; the product is high in yield and purity (the purity can reach above 83%); by the method, a new way is developed for making full use of a waste resource corn stigma.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for extracting and purifying natural phytosterols from corn silk. Background technique [0002] my country's corn production ranks second in the world. As a by-product of corn, corn silk is a huge resource and is very easy to obtain, but its development and utilization are very limited. Except for a small amount of medicine, most of them are discarded in vain, which not only causes a lot of waste, And pollute the environment. Corn silk not only has obvious pharmacological value, but also has certain dietary characteristics, and has great prospects for industrial development and utilization. The development and research of corn silk is also conducive to the reuse of waste resources and meets the requirements of national resource utilization and environmental protection. [0003] Phytosterol is a natural active substance existing in plants. Sterol...

Claims

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Application Information

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IPC IPC(8): C07J9/00
CPCC07J9/00
Inventor 张海燕张杰薄冰张珅边涛葛泰宏曹晓婉
Owner HENAN UNIVERSITY
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