Immune colored silica microsphere

Abstract

The invention relates to an immune colored silica microsphere which can be used as an alternative to immune colloidal gold to be applied to immunochromatography and overcome the problem that the immune colloidal gold with the same particle size can only display one color and is only suitable for detecting one index at present; and simultaneously, the immune colored silica microsphere is also used for overcoming the defects of easiness in leakage, difficulty in synthesis, difficulty in control of size, poor water solubility, poor biocompatibility and the like of an existing immune colored polystyrene microsphere dye. The immune colored silica microsphere provided by the invention uses the colored silica microsphere as a colored marker, amino or other active groups are modified onto the surface of the colored silica sphere by a silane coupling agent through chemical bonds to have the active groups on the surface of the silica microsphere, and an antigen or antibody is further connected onto the surface of the microsphere through the chemical bonds to prepare the immune colored silica microsphere. The immune colored silica microsphere provided by the invention has great bioactivity, uniform particle size and rich colors.

Description

technical field [0001] The invention relates to an immune silica microsphere. Background technique [0002] Immunocolor microspheres are a class of high-tech nanomaterials with broad application prospects in the fields of chemical analysis and biological analysis. 1-3 . The so-called colored microspheres refer to those nanoparticles with a particle size between 10nm and 10μm and bright colors. When they are modified with antigens or antibodies, they become immunocolored microspheres. Immunocolor microspheres have bright colors, which can increase the sensitivity of detection compared with colorless microspheres. At the same time, multi-color microspheres can realize simultaneous detection of different analytes in a sample. Currently, the only two types of immunochromatic microspheres are immunocolloidal gold 4 and immune polystyrene latex microspheres 5 . Colloidal gold is an aggregate of metal atoms, its color is usually red, and it can also display other colors by cha...

AI Technical Summary

Problems solved by technology

The disadvantage of colloidal gold is: the color of colloidal gold is not easy to control, because the color of colloidal gold is determined by the particle size, and the particle size is affected by reaction conditions and reaction time. The formation speed of colloidal gold is very fast, and it is often difficult to control its particle size. path to obtain the desired color of colloidal gold

In addition, different color colloidal gold has different particle sizes, which will affect the physical and chemical properties of colloidal gold, so colloidal gold of different colors is not suitable for simultaneous detection in one reagent

The disadvantages of colored polystyrene microspheres are: colored polystyrene microspheres are prone to pigment leakage

Dyes are often encapsulated into polystyrene microspheres by swelling. There is no strong chemical bond between the dye and polystyrene microspheres, but they are encapsulated by physical adsorption, and the dyes are easily leaked from the microspheres. , affecting its application

Although it has been reported in the literature that the dyes and polyethylene monomers are polymerized simultaneously, and the dyes are used as one of the monomers to form a polymer, most of the dyes will affect the polymerization reaction, and only a few dyes designed by the laboratory can be used to synthesize color. microspheres, the inability to utilize commercially available dyes limits the applicability of this method

Images