Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound

A carotene and compound technology, applied in the field of biochemistry, can solve the problem of low carotenoid content in oleoresin, and achieve the effect of being suitable for large-scale commercial production and convenient operation

Active Publication Date: 2015-03-25
ZHE JIANG MEDICINE CO LTD XINCHANG PHARMA FAB
View PDF10 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0040] The above-mentioned disclosed patents or applications are mainly related to the improvement of plant species to increase the content of astaxanthin in flowers, or the process of extracting calendula officinalis oleoresin with lower astaxanthin content from it. It is possible to use silica gel column chromatography to purify Marigold officinalis oleoresin, and the total carotenoid content in the finally obtained oleoresin is not high, only between 5-10wt%, for other carotenoids with lower activity not studied or defined

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound
  • Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound
  • Carotenoid compound derived from plant source and containing natural astaxanthin as well as preparation method and composition of carotenoid compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] Get Marigold officinalis extract (Calendula officinalis extract is obtained after the dry granules of Calendula officinalis are leached with a mixed solvent of propane and butane and the solvent is volatilized.) 50g (UV content 10.5wt%), added to 2500ml In absolute ethanol, heat up to 75°C under stirring, keep warm for 1.0hr to fully dissolve the side calendula extract, add dropwise 432ml of NaOH solution with a mass concentration of 45% (w / w), after the dropwise addition is completed, the reaction is mixed The alkali concentration in the solution was 2.0 mol / L, and the reaction was stirred at 75° C. for 4.0 hr. Get 5000ml of deionized water and 1000ml of ethanol into the reaction solution, after slow stirring for 1.0hr, it can be seen that dark red crystals precipitate out, filter, filter cake with ethanol and deionized water (the ratio of ethanol and deionized water is 2:1 ) mixed solution was rinsed twice, using 600ml of solvent each time, and the obtained crystals w...

Embodiment 2

[0080] Get 100g of Marigold officinalis extract (UV content 10.5wt%), join in the 3000ml glycerol, be warming up to 60 ℃ under the stirring situation, keep warm for 1.0hr and make the calendula officinalis extract fully dissolve, the mass concentration of dripping is 25% (w / w) CH 3 1000ml of ONa solution, after the dropwise addition is completed, the alkali concentration in the reaction mixture solution is 1.16mol / L, and the reaction is stirred at 60°C for 0.5hr. Get 6000ml of deionized water and 1000ml of glycerol and join in the reaction solution, after stirring at a slow speed for 1.0hr, it can be seen that dark red crystals are separated out, filter, and the filter cake uses glycerol and deionized water (the ratio of glycerol and deionized water (3:1) mixed solution was rinsed three times, using 500ml of solvent each time, and the obtained crystals were dried in a vacuum environment to finally obtain 4.8g of dark purple carotenoid crystals.

[0081] The maximum absorption...

Embodiment 3

[0086] Take 100kg of Marigold officinalis extract (UV content 10.5wt%), add it to 5000L propylene glycol, fully disperse the extract while stirring at 25°C, add dropwise 123L of KOH solution with a mass concentration of 25% (w / w) , after the dropwise addition was completed, the reaction was stirred at 25° C. for 2.5 hr. Sampling by HPLC analysis, it was found that the reaction was not complete, and continued to react for 3.5 hours. After the reaction was monitored by HPLC, 5000L of deionized water and 500L of propylene glycol were added to the reaction solution to terminate the reaction. After stirring at a slow speed for 1.0 hours, dark red crystals could be seen. , pressure filtration, the filter cake was rinsed twice with a mixture of propylene glycol and deionized water (the ratio of propylene glycol and water is 2:1), each time the solvent was 600L, and the obtained crystals were dried in a vacuum environment to finally obtain 6.8Kg Dark purple carotenoid crystals.

[00...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a high-content carotenoid compound derived from adonis amurensis as well as a preparation method and a composition of the carotenoid compound. In the high-content carotenoid compound, the content of total carotenoid is larger than 95 wt%, wherein the proportion of all-trans (3S, 3'S)-astaxanthin in the high-content total carotenoid is larger than 80 wt%. The carotenoid crystal has high purity and can be applied in the fields of human dietary supplements, food additives, feed additives and cosmetics in a variety of forms. Meanwhile, the invention further provides a preparation method of the high-content carotenoid compound from adonis amurensis, and the method is simple and reliable and is suitable for industrial production.

Description

technical field [0001] The invention relates to a natural astaxanthin-derived carotenoid compound with less impurity content and high biological activity derived from plant extracts, an extraction preparation method and a composition thereof, belonging to the field of biochemistry. Background technique [0002] The molecular formula of astaxanthin is C 40 h 52 o 4 , full name 3,3'-dihydroxy-4,4'-diketo-β,β'-carotene, widely exists in the biological world, is an important substance in carotenoids, but more than other carotenoids Carotene has stronger physiological activity. Its molecular structural formula is as follows: [0003] [0004] Astaxanthin, as a natural preparation, has very broad application and development prospects in medicine, aquaculture, food, chemical industry and other industries. [0005] Astaxanthin does not need to be converted into vitamin A when it works in the body. As a non-vitamin A source carotenoid, it has excellent functions such as prote...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24A23L1/30A23K1/16
CPCC07C403/24A61K36/71A61Q19/00A61K2800/10A61K2800/43A23K20/179A23L33/105C07C2601/14C07C2601/16A61K31/122A61K8/9789A61K2236/33A61K2236/37A61K2236/51A61K2236/53
Inventor 许新德邵斌彭永健王胜南
Owner ZHE JIANG MEDICINE CO LTD XINCHANG PHARMA FAB
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap