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Micro-channel-extraction-based bio-membrane reactor, and application of micro-channel-extraction-based bio-membrane reactor to butanol fermentation

A biofilm reactor and microchannel technology, applied in the application field of biological butanol fermentation, can solve the problems of low product concentration, increase separation cost, reduce biological reaction rate, etc., so as to avoid damage, enhance efficiency, and remove products. inhibiting effect

Active Publication Date: 2015-03-25
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: there is a phenomenon of product inhibition in the biological fermentation method. When the product reaches a certain concentration, it will inhibit the metabolism and growth of cells, greatly reduce the rate of biological reaction, make the concentration of the product not high, and increase the separation cost. , these shortcomings have seriously hindered the development of bio-fermentation industry

Method used

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  • Micro-channel-extraction-based bio-membrane reactor, and application of micro-channel-extraction-based bio-membrane reactor to butanol fermentation
  • Micro-channel-extraction-based bio-membrane reactor, and application of micro-channel-extraction-based bio-membrane reactor to butanol fermentation
  • Micro-channel-extraction-based bio-membrane reactor, and application of micro-channel-extraction-based bio-membrane reactor to butanol fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example illustrates the composition and workflow of a microchannel extractor biofilm reactor.

[0032] The biofilm reactor based on microchannel extraction consists of a hollow fiber membrane, a microchannel extractor, a feeding tank, a layering tank, an extractant storage tank and a fermenter. The fermenter and the hollow fiber membrane are connected by a silicone tube, and the feed liquid in the fermenter is pumped into the hollow fiber membrane by a peristaltic pump, and finally flows back into the fermenter from the upper outlet. The permeate outlet of the hollow fiber membrane (the cells are trapped by the hollow fiber membrane) is connected to the inlet of the microchannel extractor, and the extraction agent storage tank is connected to the other inlet of the microchannel extractor. Under the control of the plunger pump, the precise feeding is completed, and it is fully mixed inside the extractor. The discharge port of the microchannel extractor is connec...

Embodiment 2

[0036] This example illustrates the application of a microchannel continuous extraction bioreactor in biobutanol fermentation.

[0037] The specific operation steps are as follows:

[0038] The strain used in this example is Clostridium beijerinckii 8052, the extraction agent is biodiesel, the flow rate ratio of the two phases of the microchannel extractor is 1:1, and the amount of extraction agent is 2L.

[0039] Strain culture:

[0040] Clostridium butanogenes Clostridium beijerinckii 8052 was inoculated into an anaerobic bottle containing 50 mL of seed medium at an inoculum volume of 5% (v / v), passed through sterile nitrogen for 1-2 minutes, anaerobically cultured at 37°C for 12 hours, and then transferred to a bottle containing 200 Secondary activation was carried out in the anaerobic bottle of mL seed culture solution, nitrogen gas was passed for 1-2 minutes, and anaerobic cultivation was carried out at 37°C for 8 hours (the strain entered the logarithmic phase). Pu...

Embodiment 3

[0046] This example illustrates the application of a microchannel continuous extraction bioreactor in the production of butanol by fermentation.

[0047] The specific operation steps are as follows:

[0048] The strain used in this example is Clostridium beijerinckii 8052, the extraction agent is biodiesel. The feed flow rate ratio of the microchannel extractor is 1:2, and the extraction agent dosage is 2L.

[0049] Strain culture: Clostridium butanologenum Clostridium beijerinckii 8052 was inoculated into an anaerobic bottle containing 50mL seed medium at an inoculum volume of 5% (v / v), passed through sterile nitrogen for 1-2 minutes, anaerobically incubated at 37°C for 12 hours, and then transferred to a bottle containing 200mL seed medium. Secondary activation was carried out in the anaerobic bottle of the culture medium, nitrogen gas was passed for 1-2 minutes, and anaerobic cultivation was carried out at 37°C for 8 hours (the strain entered the logarithmic phase). P...

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Abstract

The invention provides a micro-channel-extraction-based bio-membrane reactor which comprises a hollow fiber membrane (A), a micro-channel extractor (B), a feed supplementing tank (C), a demixing tank (D), an extracting agent storage tank (E) and a fermentation tank (F), wherein the feed supplementing tank is connected with the fermentation tank; a feed opening and a discharge opening of the hollow fiber membrane are connected with the fermentation tank; another discharge opening of the hollow fiber membrane is connected with a feed opening of the micro-channel extractor; the extracting agent storage tank is connected to another feed opening of the micro-channel extractor; the demixing tank is connected to a discharge opening of the micro-channel extractor; the upper and lower parts of the demixing tank are connected with the extracting agent storage tank and the fermentation tank through a hose respectively. The invention further relates to application of the micro-channel-extraction-based bio-membrane reactor to butanol fermentation, and aims to improve the butanol fermentation efficiency.

Description

technical field [0001] The present invention relates to a biofilm reactor based on microchannel extraction and its application in butanol fermentation, specifically including the construction of a biofilm reactor based on microchannel extraction and its application in biobutanol fermentation, It belongs to the field of biochemical technology. Background technique [0002] Biological preparation of chemicals has the advantages of low energy consumption, safety, environmental protection, and sustainable development. In recent years, biological preparation of chemicals has become an important measure for many countries to improve energy security, reduce greenhouse gas emissions, and address climate change. Biological preparation of chemicals is often catalyzed by enzymes in the metabolic network of microbial cells to convert substrates into target products. The bottleneck technology of the biological fermentation method is that the enzymes in most biological cells are inhibite...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12P7/16
CPCC12M21/12C12M43/02C12P7/16Y02E50/10
Inventor 姜岷孔祥平贺爱永赵洁吴昊马江峰韦萍欧阳平凯
Owner NANJING UNIV OF TECH
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