Forage pediococcus acidilactici solid raw material fermentation medium and fermentation method

Abstract

The invention discloses a forage pediococcus acidilactici solid raw material fermentation medium and a fermentation method. The fermentation method comprises the following steps: inoculating pediococcus acidilactici slant strains onto a fresh MRS solid slant culture medium, carrying out constant-temperature culture to obtain solid slant strains; inoculating the solid slant strains onto a liquid seed culture medium under the aseptic condition, and carrying out constant-temperature culture to obtain liquid strains; inoculating a certain quantity of the cultured liquid strains onto the prepared solid raw material fermentation medium, standing and culturing at constant temperature, adding a heat resisting protection agent, and fully and uniformly stirring; and finally drying the fermented solid fermentation material until the content of water is less than 12%, and crushing. The method is simple in production process, low in requirements on devices, small in investment and low in production cost; pediococcus acidilactici preparations prepared by the method are stable in quality; the activity of the preparation is high; the pediococcus acidilactici preparations are easy to popularize and use in animal husbandry and aquaculture; the forage pediococcus acidilactici solid raw material fermentation medium has a great practical significance for improving the quality of animal products and aquatic products; meanwhile, the forage pediococcus acidilactici solid raw material fermentation medium has good economical and social benefits.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering fermentation, and in particular relates to a fermenting medium and a fermenting method for feed micrococcus acidophilus solid raw material. Background technique [0002] Lactic acid bacteria are the largest and earliest probiotics approved by various countries. As a beneficial bacterium (or called probiotic) for humans and animals, this bacterium is widely used in fermented milk products (such as probiotic yogurt, probiotic fermented milk drink, probiotic cheese), fermented soybean milk products, functional food ingredients and food additives (such as in infant formula milk powder, middle-aged and elderly milk powder, fruit and vegetable juice, etc.), dietary supplements (tablets, capsules, etc.) and feed microecological preparations. [0003] Pediococcus acidilactici is a lactic acid bacterium belonging to Gram-positive bacteria, non-motile, and facultatively anaerobic. At present...

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a feed micrococcus acidophilus solid raw meal fermentation medium and a fermentation method for the deficiencies of the above-mentioned prior art. The method has the advantages of simple production process, no need to invest in fermentation equipment, production Low cost and energy consumption, easy promotion and application, high number of live bacteria and high activity of the product, no discharge of three wastes, etc., solves the complex production process, high production cost and large equipment investment in the current production process of acidophilus. question