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A kind of method that enzymatic method prepares sodium gluconate

A sodium gluconate, enzymatic preparation technology, applied in the field of enzymatic preparation of sodium gluconate, can solve the problems of troublesome extraction and refining, affecting product quality and conversion rate, etc., and achieve less impurities, high equipment utilization rate, and short production cycle Effect

Active Publication Date: 2018-01-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the addition of a large amount of medium and by-products, it brings a lot of trouble to the extraction and purification in the later stage, which directly affects the quality and conversion rate of the product.

Method used

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  • A kind of method that enzymatic method prepares sodium gluconate
  • A kind of method that enzymatic method prepares sodium gluconate

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Add 20L of 40% glucose solution to a 30L tank, at the same time add 150mL (to 6000U / ml) glucose oxidase, 75 (to 100,000 U / ml) mL catalase, set the reaction pH to 6.0, and 50% Sodium hydroxide was added to adjust the pH, the reaction temperature was maintained at 38°C, the compressed air was introduced, the ventilation rate was 0.5vvm, the tank pressure was maintained at 0.2MPa, the stirring was turned on, and the speed was 250-300r / min. Reaction for about 24 hours, the residual sugar is measured <0.3%, add 50% sodium hydroxide to 3.56L, and adjust the reaction pH to 7.0, maintain for half an hour, and the catalytic reaction ends. After the reaction is over, add 600g activated carbon and heat to boiling for 5 minutes, micropore suction filtration to obtain a colorless solution, the solution obtained by suction filtration is concentrated under reduced pressure at minus 0.08MPa and temperature 80°C to a concentration greater than 70%, and the solution Fluidized bed crystall...

Embodiment 2

[0020] Add 20L of 35% glucose solution in a 30L tank, add 200mL (to 8000U / ml) glucose oxidase and 60mL (to 100,000 U / ml) catalase at the same time, set the reaction pH to 6.5, and use 50% hydrogen Sodium oxide flow is added to adjust the pH. The reaction temperature was maintained at 38°C, compressed air was introduced, the ventilation rate was 0.8vvm, the tank pressure was maintained at 0.2MPa, the stirring was turned on, and the speed was 250-300r / min. Reaction time 20 hours, measured residual sugar <0.3%, when 50% sodium hydroxide is added to reach 3.11L, the pH of the reaction is adjusted to 7.0, and the catalytic reaction ends. After the reaction is over, add 600g activated carbon and heat to boiling for 5 minutes, micropore suction filtration to obtain a colorless solution, the solution obtained by suction filtration is concentrated under reduced pressure at minus 0.08MPa and temperature 80°C to a concentration greater than 70%, and the solution Fluidized bed crystalliza...

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Abstract

The invention discloses a method for preparing sodium gluconate with an enzymatic method and belongs to the technical field of biochemistry. The method comprises steps as follows: glucose has an oxidation reaction with oxygen and water under the catalytic action of a glucose oxidase to produce gluconic acid and hydrogen peroxide, the hydrogen peroxide is decomposed into water and oxygen under the action of a catalase, and the gluconic acid has a neutralization reaction with sodium hydroxide to produce the sodium gluconate; the finished product, namely, the sodium gluconate, is obtained through enzyme deactivation, decolorization, concentration, crystallization and drying. The method can be used for producing food-grade and medicine-grade sodium gluconate and has the advantages that the operation is simple, the reaction time is short, the sodium gluconate is high in purity, little waste is produced and the like.

Description

Technical field [0001] The invention relates to an enzymatic method for preparing sodium gluconate, which belongs to the technical field of biochemistry. Background technique [0002] Sodium gluconate is a kind of polyhydroxy organic acid salt, which is widely used in chemical, food, medicine, construction and other industries. The current production methods of sodium gluconate mainly include four methods: homogeneous chemical oxidation, electrolytic oxidation, heterogeneous catalytic oxidation and biological fermentation. The homogeneous chemical oxidation method uses crystalline glucose as a raw material, dissolves the crystalline glucose in water and then adds a catalyst. At a certain reaction temperature, sodium hypochlorite solution is added and lye is added dropwise to make the equilibrium system move toward the direction of sodium gluconate generation. After the reaction, the sodium gluconate product can be obtained by concentrating and crystallization. The process is ea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/58
CPCC12P7/58
Inventor 余晓斌肖成建顾秋亚
Owner JIANGNAN UNIV
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