Staining method for thrombus compositions and application of staining method

Abstract

The invention discloses a staining method for thrombus compositions and application of the staining method, and relates to the technical field of medical science, in particular to a chemical staining method for the thrombus compositions, and a method for identifying the thrombus compositions and authenticating thrombus properties. The disclosed staining method for the thrombus compositions comprises the following steps: dewaxing and hydrating a paraffin tissue section in sequence; performing mordant staining on the dewaxed and hydrated paraffin tissue section by using halotrichite aqueous solution, staining the paraffin tissue section which is subjected to the mordant staining by using hematoxylin, picric acid-orange G solution, and carmine-magenta solution, and differentiating colors by using phosphotungstic acid, so that different compositions in a thrombus present different colors. The invention also discloses the method for identifying the thrombus compositions and authenticating the thrombus properties by applying the staining method; the thrombus compositions can be identified and the thrombus properties can be authenticated effectively according to different colors of the different compositions in the thrombus and according to the colors and morphology.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a chemical staining method for thrombus components and a method for identifying thrombus components and identifying thrombus properties. Background technique [0002] In the process of medical specimen diagnosis and animal experiments, it is often necessary to identify thrombus. This thrombus identification can be completed through pathological detection, that is, through the staining and identification of thrombus to identify the components of thrombus, including red blood cells, white blood cells, platelets and fibers in thrombus. Protein or fibrin tissue; identify the nature of thrombus, including red thrombus, white thrombus, mixed thrombus. [0003] The staining and identification techniques involving thrombus in the prior art mainly include: (1) Hematoxylin-Eosin staining (Hematoxylin-Eosin staining), referred to as HE staining: it is the most basic and most commonly used ...

AI Technical Summary

Problems solved by technology

The hematoxylin in the dye solution of HE staining is a basic dye, which makes the chromatin in the nucleus and the ribosome in the cytoplasm stain purple blue; while the eosin in the dye solution is acidic, which makes the cytoplasm and extracellular matrix The components in the dye are dyed red, so the advantage of HE staining is that only two dyes, hematoxylin and eosin, are used, the staining steps are fewer, and the staining process is faster. It only takes 1-2 hours. The cells are clearly displayed; the disadvantage is that for thrombus, except for the white blood cell nucleus, which is stained blue, other components are stained red, including red blood cells, white blood cell cytoplasm, platelets, and cellulose components in the thrombus. These components cannot be determined accurately Distinguish; (2) Phosphotungstic acid hematoxylin staining method (PTAH method): after oxidizing and bleaching the sections with potassium permanganate and oxalic acid, the specimens were dipped with phosphotungstic acid hematoxylin dye for 24 hours to make the fibers in the thrombus components The agglomerates of the plain network structure are dyed blue-purple, which is beneficial to distinguish thrombus or even microthrombus. Therefore, the advantage of PTAH method is that it can identify thrombus, especially microthrombus, by identifying cellulose. The disadvantage is that the staining time is long, and it cannot identify the thrombus. Other cell components include red blood cells, white blood cells, and platelets; (3) Martius Scarlet Blue (MSB) staining method: Cellulose MSB staining method is respectively processed by lapis lazuli blue liquid staining, hematoxylin staining liquid staining, Matthew Yellow liquid staining, and bright crystal purple scarlet Dyeing liquid dyeing, phosphotungstic acid, and aniline blue liquid dyeing treatment can stain the red blood cells in the thrombus into yellow, the white blood cell nuclei into blue-black, and the cellulose into golden yellow to bright red. The staining results are colorful and poor in contrast; (4) blood cell staining: for example, peroxidase staining makes blue localize in the cytoplasm, periodic acid-Schiff staining purple makes red localize the glycogen substances in the cytoplasm, but does not recognize the cellulose in the thrombus