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Novel helicobacter pylori detection method and device as well as application thereof

A Helicobacter pylori detection method technology, applied in the detection field of Helicobacter pylori, can solve the problem of low detection sensitivity of infrared spectrometer

Inactive Publication Date: 2015-03-25
SUZHOU QINGSHAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The technical problem to be solved in the present invention is, for the Helicobacter pylori in the prior art 14 The radiotoxicity of the C detection method causes the cancer risk of the subjects, and the environmental pollution of the production tracer and the traditional method 13 C detection uses an infrared spectrometer to detect defects with low sensitivity, and provides a technology that uses wavelength-scanning cavity ring-down spectroscopy (WS-CRDS) to improve 13 The accuracy of the C test, a new type of application that reduces the amount of isotope reagents and test costs 13 Method for detecting Helicobacter pylori by tracer breath of C-labeled urea, device using the method and application of the method in clinical detection

Method used

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  • Novel helicobacter pylori detection method and device as well as application thereof
  • Novel helicobacter pylori detection method and device as well as application thereof
  • Novel helicobacter pylori detection method and device as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Example 1 The detection process of the double-mirror optical cavity ring-down spectrum detection device

[0072] Schematic diagram of the detection process and the characteristic spectrum of the double-mirror optical cavity ring-down spectrum detection device as shown in figure 2 , image 3 with Figure 4 shown

[0073]Firstly, the threshold value of the light sensor is set to 1 / 100,000. When there is no exhaled gas, the laser diode generates pulsed single-frequency laser light, and the beam enters the chamber equipped with two high-reflectivity double-reflectors for continuous circular reflection. The number of continuous reflections is 50,000~times. The photoelectric sensor senses the laser light leaked from any interface and records its signal, which is output in proportion to the light intensity in the chamber. When the signal sensed by the light sensor reaches the set threshold, the continuous laser is turned off, and the laser remaining in the chamber continu...

Embodiment 2

[0076] Example 2 Detection process of ring-down spectrum detection device with three-mirror optical cavity

[0077] The schematic diagram of the detection process and characteristic spectrum of the three-mirror optical cavity ring-down spectrum detection device is as follows Figure 5 with Image 6 shown.

[0078] First, set the threshold value of the optical sensor to one hundred thousandth. When there is no exhaled gas, the laser generates pulsed single-frequency laser light, and the beam enters the resonant chamber equipped with two high-reflectivity double-reflectors for continuous circular reflection. The number of continuous reflections is 150,000~times. Other steps are the same as the detection process of the ring-down spectrum detection device with double-mirror optical cavity in embodiment 1.

Embodiment 3

[0079] Embodiment 3 uses three-mirror optical cavity ring-down spectroscopy to detect the patient's exhalation 13 C concentration to determine the level of Hp in the patient's stomach

[0080] Let patients take 13 C-labeled urea 13 C (NH 2 ) 2 preparations, such as 1 capsule, the urease contained in the Helicobacter pylori in the patient's stomach can catalyze 13 C-urea breaks down and finally passes through the lungs to 13 CO 2 The form exhales. After 30 minutes, the patient's breath was sampled, and the CO 2 The partial pressure is 0.01-1.0x10 -6 , the total gas pressure is 1-1.0x10 -6 atm. Using a three-mirror optical cavity ring-down spectroscopy device to obtain CO 2 The characteristic spectral line of the resonant chamber unit is calculated by integrating 13 CO 2 with 12 CO 2 The characteristic peaks can be obtained by taking 13 Patient Exhalation of C-Labeled Reagent 13 CO 2 / 12 CO 2 ratio, with unused 13 C tracer measured 13 CO 2 / 12 CO 2 Sc...

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Abstract

The invention provides a method for carrying out in vitro diagnosis on concentration of helicobacter pylori in a stomach by taking a wavelength scanning-cavity ring down spectroscopy (WS-CRDS) as a detection technology and taking urea marked by carbon-13 isotope as a tracer agent through detecting carbon-13 abundance change of carbon dioxide in breathing air of a patient. A measurement technology comprises the following steps: leading generated laser into a light reflection unit to be circulated and reflected until small amount of laser strength leaked by the light reflection unit reaches a threshold value; stopping inputting the laser until light cavity attenuation is zero; inputting CO2-containing gas exhaled by the patient into a resonance cavity unit to accelerate oscillation of laser; detecting a light intensity resonance difference in a resonance cavity by using the laser with adjustable wavelength; calculating a time difference of inputting target gas CO2 and hollow cavity oscillation to form a characteristic spectrum line; integrating characteristic spectrum peaks of 13CO2 and 12CO2; and measuring carbon-13 abundance of the target gas CO2; and comparing delta13CO2 of the carbon-13 abundance in the CO2 before and after taking the urea marked by the carbon-13 isotope as the tracer agent, wherein a high-confidence coefficient clinical statistical result shows that a direct ratio is formed by the delta13CO2 and Hp content. The method is accurate, flexible, environmentally friendly and economical, and is suitable for clinical large-scale application.

Description

[0001] technical field [0002] The present invention relates to the detection technique of Helicobacter pylori, in particular to a detection technology of Helicobacter pylori 13 C marks breath detection method, detection device and application thereof. Background technique [0003] Helicobacter pylori (Hp for short) is a unipolar, multi-flagellate, blunt-rounded, spirally curved bacterium with a length of 2.5-4.0 μm and a width of 0.5-1.0 μm. The bacterium often has a typical spiral or arc shape on the surface of gastric mucosal epithelial cells; when growing on a solid medium, in addition to the typical shape, sometimes it can appear rod-shaped or spherical. Helicobacter pylori is a microaerophilic bacterium that requires 5-8% oxygen in the environment and cannot grow in the atmosphere or in an absolutely anaerobic environment. Hp was first discovered by Barry J. Marshall and J. Robin Warren. In 1982, Marshall personally conducted human experiments and swallowed one bill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
Inventor 胡军王恩泽
Owner SUZHOU QINGSHAN BIOTECH
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