Semi-quantitative nitrite rapid detecting composition and application thereof

A technology of nitrite and composition, applied in the field of chemical detection, can solve problems such as unavailable test paper products, inaccurate test results by test paper method, and easy oxidation

Active Publication Date: 2015-03-25
贝因美(杭州)食品研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are two main problems with this method: one is that standardized test paper products cannot be obtained, and the amount of water absorbed by the filter paper of the chromogenic agent during product processing and the loss in the drying process cannot be unified; the other is that the chromogenic agent is attached to the paper In contact with air in the fiber, it is easy to oxidize
These reasons have caused the inaccurate test results of the test paper method and the defects that the product is not easy to preserve.

Method used

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  • Semi-quantitative nitrite rapid detecting composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1: composition of the present invention and freeze-dried solid thereof

[0049] Core material: Mix 0.4% p-aminobenzenesulfonic acid solution and 0.2% naphthaleneethylenediamine hydrochloride solution, the volume ratio is 1:1;

[0050] Wall material: 1 part of sodium pyrophosphate, 3 parts of mixture of phospholipid and caprylic glycerin, 30 parts of whey protein, 20 parts of lactose, 1 part of 0.1% tartrate, 50 parts of deionized water;

[0051] Composition: the core material and the wall material are mixed at a volume ratio of 1:1 to obtain a composition;

[0052] Freeze-dried solid: freeze-dried the composition to obtain a freeze-dried solid.

Embodiment 2

[0053] Embodiment 2: composition of the present invention and freeze-dried solid thereof

[0054] Core material: Mix 0.4% p-aminobenzenesulfonic acid solution and 0.2% naphthaleneethylenediamine hydrochloride solution, volume ratio 1:3;

[0055] Wall material: 5 parts of sodium pyrophosphate, 1 part of mixture of phospholipid and caprylic glycerin, 15 parts of whey protein, 50 parts of sucrose, 3 parts of 0.1% tartrate, 200 parts of deionized water;

[0056] Composition: the core material and the wall material are mixed at a volume ratio of 1:20 to obtain the composition;

[0057] Freeze-dried solid: freeze-dried the composition to obtain a freeze-dried solid.

Embodiment 3

[0058] Embodiment 3: composition of the present invention and freeze-dried solid thereof

[0059] Core material: Mix 0.4% p-aminobenzenesulfonic acid solution and 0.2% naphthaleneethylenediamine hydrochloride solution, volume ratio 1:5;

[0060] Wall material: 3 parts of sodium pyrophosphate, 5 parts of mixture of phospholipid and caprylic glycerin, 20 parts of soybean protein, 35 parts of lactose, 2 parts of 0.1% tartrate, 100 parts of deionized water;

[0061] Composition: the core material and the wall material are mixed at a volume ratio of 1:10 to obtain a composition;

[0062] Freeze-dried solid: freeze-dried the composition to obtain a freeze-dried solid.

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PUM

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Abstract

The invention relates to the field of chemical testing, and particularly discloses a semi-quantitative nitrite rapid detecting composition and application thereof. The composition comprises a core material and a wall material; the core material comprises 0.4 percent sulfanilic acid and 0.2 percent N-ethylenediamine; the wall material comprises a mixture of sodium pyrophosphate, phospholipid and capric triglyceride, soy protein or whey protein, lactose or sucrose, 0.1 percent tartrate and deionised water. According to the composition, the core material of the sulfanilic acid solution and the N-ethylenediamine solution is embedded into the wall material, so that the wall material can effectively carry and protect the core material, and can quickly disintegrated when the wall material is in contact with milk and dairy products, the reaction is quick, and the accuracy and sensitivity of the detection are high.

Description

technical field [0001] The invention relates to the field of chemical detection, in particular to a composition for semi-quantitative rapid detection of nitrite and an application thereof. Background technique [0002] Nitrite is an important detection index for raw materials of milk and dairy products, especially milk powder products. my country's national standard GB 2762 clearly stipulates the limits of nitrate and nitrite in milk powder. [0003] The toxicology of nitrite is classified as a highly toxic substance. After entering the blood, it turns oxyhemoglobin into methemoglobin, thereby losing its oxygen-carrying capacity, leading to tissue hypoxia, purpura, and teratogenic and carcinogenic effects. The harm of nitrite is described by WHO / FAO as strong toxicity, oral LD50=200mg / kg body weight for mice, 0.3g-0.5g for adults and ADI=0.2mg / kg body weight. [0004] Normal raw milk does not contain nitrite, and nitrite in the ecosystem mainly comes from surface water, ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 储小军富鑫华家才姜艳喜石红张素文
Owner 贝因美(杭州)食品研究院有限公司
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