Preparation method of electrochemical mark-free immunosensor of gastric cancer tumor marker

A technology of tumor markers and immunosensors, which is applied in the field of preparation of electrochemical label-free immunosensors, can solve problems such as cumbersome operations, and achieve the effects of simple preparation, convenient operation, and high selectivity detection

Inactive Publication Date: 2015-04-08
UNIV OF JINAN
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  • Abstract
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Problems solved by technology

[0004] The research on the application of electrochemical immunosensors to detect gastric cancer tumor markers is o

Method used

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  • Preparation method of electrochemical mark-free immunosensor of gastric cancer tumor marker

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Embodiment 1

[0031] Example 1 Preparation method of electrochemical gastric cancer tumor marker label-free immunosensor:

[0032] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 5 uL of titanium dioxide nanoparticle sol TiO on the electrode surface 2 NPs, after drying at room temperature, drop-coated 5 μL of gold and silver nanorod sol AuAg NRs, and dried at room temperature;

[0033](2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 5uL 0.01 mol / L chloropalladium acid solution H on the surface 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 30 seconds to obtain TiO 2 NPs-loaded gold-silver-palladium alloy nanorod material AuAg-Pd NDRs modified working electrode, dried at room temperature;

[0034] (3) Wash the electrode obtained in step (2) with ultrapure water, and after drying at room temperature, drop-coat 5 uL of thionine solution...

Embodiment 2

[0046] Example 2 Preparation method of electrochemical gastric cancer tumor marker label-free immunosensor:

[0047] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 8 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 8 μL of AuAg NRs and dried at room temperature;

[0048] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 8uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 60 seconds to obtain TiO 2 NPs-loaded AuAg-Pd NDRs modified working electrode, dried at room temperature;

[0049] (3) Clean the electrode obtained in step (2) with ultra-pure water, dry it at room temperature, drop-coat 8 uL TH on the surface, and dry it at room temperature;

[0050] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 8 uL CHS on the surface, and dry it at ...

Embodiment 3

[0057] Example 3 Preparation method of electrochemical gastric cancer tumor marker label-free immunosensor:

[0058] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 10 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 10 μL of AuAg NRs and dried at room temperature;

[0059] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 10uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 90 seconds to obtain TiO 2 NPs-loaded AuAg-Pd NDRs modified working electrode, dried at room temperature;

[0060] (3) Wash the electrode obtained in step (2) with ultrapure water, and after drying at room temperature, drop-coat 10 uL TH on the surface, and dry at room temperature;

[0061] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 10 uL CHS on the surface, and ...

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Abstract

The invention discloses a preparation method of an electrochemical mark-free immunosensor of a gastric cancer tumor marker, and belongs to the field of a novel nano functional material and a biosensor. Firstly, a titanium dioxide-noble metal composite material is directly synthesized on an electrode on a titanium dioxide nano particle substrate by using a photoelectrical chemical synthesis method; furthermore, the electrochemical mark-free immunosensor of the gastric cancer tumor marker, which has the advantages of low cost, high sensitivity, good specificity, rapidness in detection and simplicity in operation, is prepared.

Description

Technical field [0001] The present invention involves a preparation method for detecting the electrochemical immune sensor for the detection of gastric cancer tumor markers.It belongs to the new type of nano -functional materials and biosensor technology. Background technique [0002] Due to its high sensitivity, good specificity, and simple operation, electrochemical immune sensors are widely used in the field of clinical analysis and environmental testing. They can generally be divided into two types: labeling and unparalleled.The requirements for the detection environment are stricter and the operation is relatively cumbersome. Therefore, no standard electrochemical immune sensor has a broader application prospect.To build a standard electrochemical chemical immune sensor, the key technology is to improve the fixed volume of modified electrode antibodies and the signal response speed and size of the signal of the test base.Titanium dioxide nano particles TIO 2 NPS is a kind of...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N27/26
CPCG01N27/26G01N33/57446
Inventor 张勇吴丹魏琴杜斌范大伟胡丽华
Owner UNIV OF JINAN
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