Method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography

A technology of high-speed countercurrent chromatography and black fruit wolfberry, which is applied in the direction of organic chemistry, etc., to achieve the effect of simple process, convenient operation and good repeatability

Active Publication Date: 2015-04-15
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, no one has used HSCCC technology to separate and purify petunienin from Lycium barbarum

Method used

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  • Method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography
  • Method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography
  • Method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Preparation of crude extract

[0024] Add 100mL of methanol (2% formic acid) to 100g of dried fruit of Lycium barbarum to extract for 24h, and repeat 3 times. After 3 times of extracts were combined, filter paper was used to remove insoluble matter, protein, and polysaccharides. After the filtrate was collected, the methanol was concentrated by a rotary evaporator, and the extract was redissolved in distilled water (2% formic acid) and applied to D-101 macroporous resin (50mm×800mm). The macroporous resin was pretreated with methanol and water. After loading the sample, flush the column with 3 times the column volume of distilled water to remove sugars, amino acids and other impurities. Then wash the column with methanol, collect the effluent, concentrate and dry under reduced pressure to obtain the crude extract.

[0025] 2. Preparation of Lycium barbarum anthocyanins by high-speed countercurrent separation

[0026] Prepare a two-phase solvent system of methyl t...

Embodiment 2

[0036] 1. Preparation of crude extract

[0037] Add 100mL of methanol (2% formic acid) to 100g of dried fruit of Lycium barbarum to extract for 24h, and repeat 3 times. After 3 times of extracts were combined, filter paper was used to remove insoluble matter, protein, and polysaccharides. After the filtrate was collected, the rotary evaporator was concentrated to remove methanol, and the extract was reconstituted with distilled water (2.% formic acid) and applied to D-101 macroporous resin (50mm×800mm). The macroporous resin was pretreated with methanol and water. After loading the sample, flush the column with 3 times the column volume of distilled water to remove sugars, amino acids and other impurities. Then wash the column with methanol, collect the effluent, concentrate and dry under reduced pressure to obtain the crude extract.

[0038] 2. High-speed countercurrent separation

[0039] Prepare a two-phase solvent system of methyl tert-butyl ether: n-butanol: acetonitr...

Embodiment 3

[0042] 1. Preparation of crude extract

[0043] Add 100mL of methanol (2% formic acid) to 100g of dried fruit of Lycium barbarum to extract for 24h, and repeat 3 times. The extraction process was carried out at room temperature and protected from light. After 3 times of extracts were combined, filter paper was used to remove insoluble matter, protein, and polysaccharides. After the filtrate was collected, the methanol was concentrated by a rotary evaporator, and the extract was redissolved in distilled water (2% formic acid) and applied to D-101 macroporous resin (50mm×800mm). The macroporous resin was pretreated with methanol and water. After loading the sample, flush the column with 3 times the column volume of distilled water to remove sugars, amino acids and other impurities. Then wash the column with methanol, collect the effluent, concentrate and dry under reduced pressure to obtain the crude extract.

[0044] 2. High-speed countercurrent separation

[0045] Prepare...

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Abstract

The invention provides a method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography. With utilization of the high-speed counter-current chromatography and adopting of a reasonable solvent system, the high-purity petunidin is obtained from lycium ruthenicum murray; the method does requires no use of a solid-phase carrier, has no irreversible adsorption, and separates and prepares petunidin anthocyanin with no loss of samples, no pollution, high efficiency, speediness and low cost; the purity of the obtained petunidin can reach more than 97%. The method has the advantages of simple process, convenient operation, safety and good reproducibility, and can be used for industrialized production.

Description

technical field [0001] The invention relates to a method for extracting and isolating petunia from black fruit Lycium barbarum by using HSCCC technology. Background technique [0002] The characteristics of drought resistance, salt and alkali resistance, and sand burial resistance of black wolfberry make it a rare shrub species for windbreak and sand fixation, water and soil conservation, and soil improvement. According to the Tibetan medical classics "Jingzhu Materia Medica" and "Four Medical Classics", it is recorded that black fruit wolfberry is sweet in taste, flat in nature, clears heart heat, and is used to treat heart fever, heart disease, irregular menstruation, and menopause. "Uyghur Medicine Chronicle" records: black fruit wolfberry fruit can treat diseases such as urethral calculus, ringworm and scabies, bleeding gums, etc., and it is used as nourishing and strong, improving eyesight and antihypertensive medicine among the people. The use of Nitraria tanguta frui...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 丁晨旭张秋龙胡娜马涛李文聪索有瑞
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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