Crushing method of thallus cells

Abstract

The invention discloses a crushing method of thallus cells. The crushing method comprises the following steps: (a), freezing, namely collecting a thallus fermentation liquid, pumping the thallus fermentation liquid into a water distribution plate of a flake ice machine, and sprinkling the thallus fermentation liquid in the water distribution plate onto an icing surface in the flake ice machine to form an ice layer; and (b), melting and crushing, namely scraping off bacterium liquid borneol from the icing surface by virtue of an ice cutter and dropping borneol into an ice bin, firstly freezing at -5 DEG C to -90 EDG C for 1-10 hours, and then melting at 10-60 DEG C to obtain crushed thallus cell fluid. According to the crushing method disclosed by the invention, the thallus fermentation liquid is frozen by virtue of the flake ice machine to ensure that the thallus fermentation liquid is in large area contact with the icing surface in the flake ice machine, the purposes that the thallus fermentation liquid can be rapidly prepared into borneol and is extremely easy to melt can be achieved, the flake ice machine is applied to the biological fermentation industry for the first time, the crushing efficiency of the thallus cells can be effectively improved, the crushing rate can reach 95%, and the retaining rate of enzyme activity can reach 97%.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for crushing bacterial cells. Background technique [0002] Most Escherichia coli genetically engineered bacteria belong to intracellular enzymes, so it is necessary to break the bacterium cells first to release the enzymes in the cells, and then use the biocatalytic performance of the enzymes. In the prior art, the method of breaking bacterium cells There are many kinds, which can be roughly divided into two categories: mechanical method and non-mechanical method according to the application of external force: mechanical method mainly includes pressing method (solid shearing action) and high-pressure homogenization method (liquid shearing action) Non-mechanical methods mainly include chemical method, autolysis method, enzymatic method and freeze-thaw method. In actual production, no matter which crushing method is used, it is necessary to ensure that the impact on the acti...

AI Technical Summary

Problems solved by technology

In actual production, no matter which crushing method is used, it is necessary to ensure that the impact on the activity of the target product enzyme is minimized. At present, the crushing technology suitable for large-scale industrial applications is mainly mechanical, but its high shear force is easy to cause sensitivity. Inactivation of sexual enzymes. In addition, the chemical method of disrupting bacterial cells is mainly used on a small scale in the laboratory, and this method is easy to introduce impurities, and the cost of enzymatic hydrolysis is relatively high. Therefore, these two methods are not suitable for industrial applications. In comparison, the freeze-thaw method is more suitable for the large-scale application of crushed bacterial cells after production verification. The bacterial cells are first refrigerated and then melted in a refrigerator, so that the formation of ice particles in the bacterial cells increases the salt concentration of the remaining cell fluid, which can cause the cells to swell and break. However, this method not only consumes a lot of manpower, but also has a high cost of crushing. Therefore, it is very important to seek a low-cost and fast method of disrupting bacterial cells, which is very important for the use of enzymes in bacterial cells to achieve catalysis