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Method for electrochemical device to utilize corn cob hydrolysate

A corn cob hydrolyzate, electrochemical technology, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of limiting the development of succinic acid, unsustainable development, and low production of succinic acid. Achieve the effect of easy operation, simple method and low pollution

Inactive Publication Date: 2015-04-22
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the chemical method mainly uses non-renewable petroleum resources as raw materials, which has high cost, serious pollution and unsustainable development, which seriously limits the development of succinic acid
The yield of succinic acid produced by biological method is relatively low

Method used

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  • Method for electrochemical device to utilize corn cob hydrolysate
  • Method for electrochemical device to utilize corn cob hydrolysate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for utilizing corncob hydrolyzate in an electrochemical device, comprising the following steps:

[0024] Step 1, degrading corncob by dilute acid method: add 5mL dilute H with a volume fraction of 2% per gram of corncob 2 SO 4 , 121°C, sterilized for 20 minutes, decolorized with activated carbon, filtered with suction, and rotary steamed.

[0025] Step 2, prepare seed culture medium, catholyte, anolyte, seed washing liquid, wherein, seed culture medium (g·L -1 ) including glucose 10 (divided), yeast extract 5, corn steep liquor 5, NaHCO 3 10, NaH 2 PO 4 2H 2 O 9.6, K 2 HPO 4 ·3H 2 O 15.5; catholyte (g L -1 ) including yeast extract 5, NaH 2 PO 4 2H 2 O 8.5, Na 2 HPO 4 12H 2 O 31.51, NaHCO 3 10; neutral red (g L -1 ) 0.0288; anolyte (g L -1 ) including Na 2 HPO 4 12H 2 O 2.51, NaH 2 PO 4 2H 2 O 2.81, NaCl 1.45; DTT (g L -1 ) 0.2; seed lotion (g L -1 ) including Na 2 HPO 4 12H 2 O 5 , NaH 2 PO 4 2H 2 O 5.6, DTT 0.15.

[0026] ...

Embodiment 2

[0031] Step 1 to step 4 are the same as Example 1.

[0032] Step 5, first centrifuge the fermented bacteria at 10000rpm to remove the supernatant, wash 4 times with the seed washing solution and centrifuge, then insert the bacteria into the catholyte, then add 0.0288g / L neutral red and 10g / L corn cob The hydrolyzate is connected to the catholyte, mixed evenly, and finally connected to the cathode cavity of the bioelectrochemical reactor. Then 0.2g / L DTT was added to the anolyte, mixed evenly, and then connected to the anode cavity of the bioelectrochemical reactor.

[0033] Step 6, connect the bioelectrochemical reactor to electricity, keep the constant voltage at -0.8V, and pass CO into the cathode chamber 2 0.02 L / (min·L), the temperature was controlled at 37°C for 12 hours of fermentation.

Embodiment 3

[0035] Step 1 to step 4 are the same as Example 1.

[0036] Step 5, first centrifuge the fermented bacteria at 12000rpm to remove the supernatant, wash with the seed washing solution for 5 times and centrifuge, then insert the bacteria into the catholyte, then add 0.0288g / L neutral red and 15g / L corn cob The hydrolyzate is connected to the catholyte, mixed evenly, and finally connected to the cathode cavity of the bioelectrochemical reactor. Then 0.2g / L DTT was added to the anolyte, mixed evenly, and then connected to the anode cavity of the bioelectrochemical reactor.

[0037] Step 6, connect the bioelectrochemical reactor to electricity, keep the constant voltage at -1.2V, and pass CO into the cathode chamber 2 0.02 L / (min·L), the temperature was controlled at 37°C for 24 hours of fermentation.

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Abstract

A method for an electrochemical device to utilize corn cob hydrolysate comprises the following steps that corn cobs are added into dilute sulphuric acid to be disinfected, and the corn cob hydrolysate is obtained after activated carbon decoloration, extraction filtration and rotary evaporation; a seed medium, catholyte, anolyte, seed washing liquid, an electron medium and DTT are prepared; disinfection is carried out on the prepared reagents, a bio-electrochemical reactor and a serum bottle; the disinfected serum bottle is fetched, the seed medium is added into the serum bottle, CO2 is led into the serum bottle, and actinobacillus succinogenes is added to be cultured to serve as a fermentative strain; centrifugation is carried out on the fermentative strain to remove the supernatant, a strain body is obtained after washing and secondary centrifugation and is added into the catholyte, then the electron medium and the corn cob hydrolysate are added into the catholyte to be mixed evenly and are connected to a cathode cavity of the bio-electrochemical reactor, and then the DTT is added into the anolyte to be mixed evenly and added into an anode cavity; chemical CO2 is led into the cathode cavity, and a product is obtained after fermentation at 37 DEG C. The method is simple, the yield is high, and the method is suitable for industrialized operation.

Description

technical field [0001] The invention belongs to the field of electrochemical biological method fermentation, and in particular relates to a method for utilizing corncob hydrolyzate by an electrochemical device. Background technique [0002] China is a large agricultural country, and corn is one of the three major food crops, forming a large amount of biomass raw materials such as straw and corncobs. At present, the level of carp corn cobs in China is relatively low, and most of them are used as fuel, resulting in a serious waste of resources. Corn cobs mainly contain cellulose, hemicellulose, and lignin, which can be degraded to form fermentable sugars. There are many technologies for corncob treatment, including steam explosion technology, dilute acid pretreatment technology and acid enzyme hydrolysis technology. Among them, dilute acid pretreatment technology is relatively mature. [0003] Succinic acid, also known as succinate acid, is a common natural organic acid a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/46C12R1/01
CPCC12P7/46C12P2201/00
Inventor 陈可泉赵艳曹伟佳王震何珣欧阳平凯
Owner NANJING UNIV OF TECH
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