Application of vanadate to improvement of soil cultivated plant drought resistant stress

A soil culture plant and vanadate technology, which is applied in the field of promoting plant resistance to drought stress, can solve the problems of environmental damage and high cost, and achieve the effects of increasing water content, low cost and simple operation

Inactive Publication Date: 2015-04-29
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of these are to alleviate drought by changing the external environment, and some methods are costly and detrimental to the environment. Therefore, it is neces...

Method used

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  • Application of vanadate to improvement of soil cultivated plant drought resistant stress
  • Application of vanadate to improvement of soil cultivated plant drought resistant stress
  • Application of vanadate to improvement of soil cultivated plant drought resistant stress

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Sodium vanadate improves soil culture broad bean drought resistance experiment, comprises the steps:

[0027] (1) The experimental material is broad bean seedlings

[0028] Take the local broad beans sown in autumn in Yunnan, wash them with sterile water, put them in a tray containing absorbent paper, add 40mL of sterile water to the tray to soak the absorbent paper, and culture them in dark at 25°C to accelerate germination; Seeds are sown in special earth-culture small pots mixed with red soil and perlite at a volume ratio of 6:1. Three seeds are placed in each small pot, watered once every two days, and used for this experiment when the seedlings grow to four pairs of leaves. ;

[0029] (2) Prepare different concentrations of sodium vanadate (30, 60 and 100 μmol / L) treatment solutions;

[0030] (3) Irrigate broad bean seedlings with the treatment solution of the above concentration, each treatment requires 100ml of treatment solution, and water treatm...

Embodiment 2

[0033] Embodiment 2: adopt sodium vanadate (100 μ mol / L) treatment liquid to treat broad beans, verify that sodium vanadate water-retaining agent has effect on broad bean stomatal conductivity, transpiration rate, H 2 o 2 content and the effect of water content.

[0034] Figure 5 , 6 Showing the stomatal conductance of leaves of plants treated with sodium vanadate at a concentration of 100 μmol / L ( Figure 5 A), transpiration rate ( Figure 5 B) and H 2 o 2 content. It can be seen from the figure that after one day of treatment, compared with the control group, the stomatal conductance and transpiration rate of the 100 μmol / L sodium vanadate treatment group were significantly lower, while the H 2 o 2 content has increased.

[0035] Figure 7 , 8 Shows the stomatal conductance of plant leaves treated with sodium vanadate at a concentration of 100 μmol / L for three days ( Figure 7 A), transpiration rate ( Figure 7 B) and H 2 o 2 ( Figure 8 A) content and leaf ...

Embodiment 3

[0037] Example 3: The broad beans treated with sodium vanadate (100 μmol / L) treatment solution were used to verify that sodium vanadate has effect on broad bean hydrogen pump activity and plasma membrane H + -ATPase activity comprising the steps of:

[0038] (1) Determination of hydrogen pump activity

[0039] A. After being treated with 100 μmol / L sodium vanadate, 0.5 g of broad bean leaves with different treatments were taken, quickly frozen in liquid nitrogen, ground into powder by liquid nitrogen, and then added with 1 mL of plasma membrane extract (0.25 mol / L sorbitol, 1 mmol / L EDTA, 5 mmol / L MgSO 4 , 10 mmol / L Tris-HCl pH7.4); the homogenate was centrifuged at 9,000g at 4°C for 20 min to remove the precipitate, and the supernatant was centrifuged at 30,000g at 4°C for 1 h; the precipitate was collected and suspended in 0.5ml of the above extract , resulting in plasma membrane protein;

[0040] B. Determination of plasma membrane protein concentration by Bradford meth...

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Abstract

The invention discloses an application of vanadate to improvement of soil cultivated plant drought resistant stress. In the use process, a vanadate solution with the concentration being 30 to 100mu mol/L is used for treating potted broad beans, water treatment is used as the blank control, after one day and three days after the treatment, the growth condition of the plants is observed, and various physiological indexes of the plants are measured. Experimental results show that through comparison with control, after vanadate treatment, the pore conductivity, the transpiration rate, the H<+>- pump activity and plasma membrane H<+>- ATPase activity of the plants and the H2O2 content are improved, and the water content of leaves is increased, and the physiological activities of the plants are favorably weakened through changes, so that the whole water content of the plants is enabled to be in a relatively stable level. Experimental results show that vanadate has an application prospects in the aspect of improving the drought resistant stress capability of soil cultivated plants.

Description

technical field [0001] The invention belongs to the field of promoting the resistance of plants to drought stress, and relates to the new use of vanadate, an inorganic chemical reagent, in improving the response of soil culture plants to drought stress. Background technique [0002] In production, droughts with high frequency and long duration often occur, causing immeasurable losses to crop production and seriously affecting their quality; the annual loss of food production caused by drought in the world is almost equal to that caused by all other environmental factors The sum of losses, currently facing the global food crisis, how to improve crop yield under drought conditions has become one of the important topics of scholars at home and abroad. my country is one of the most water-scarce countries in the world, and the arid and semi-arid areas account for about one-half of the country's land area. According to statistics, more than 70% of my country's crop area is affect...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01C1/00
CPCA01G7/06A01C1/00
Inventor 陈丽梅吴怀胜
Owner KUNMING UNIV OF SCI & TECH
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