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Preparation method and application of cell-loading three-dimensional support

A three-dimensional scaffold and cell technology, which is applied in the field of cell printing and biomaterials, can solve the problems of cell damage, large shear stress, and susceptibility to environmental influences, and achieve the effects of reducing damage, easy operation, and reducing damage

Active Publication Date: 2015-04-29
深圳尤尼智康医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cell printing output technologies mainly include inkjet printing (piezoelectric volume-driven and thermal bubble), laser direct writing, laser-induced transfer, electrostatic spraying, focused ultrasonic jetting, micro-extrusion, etc. The feasibility and reliability of traditional cell printing All aspects have been verified, but there are still limitations in how to maintain cell viability: when printing, most mammalian cells are relatively fragile and susceptible to environmental influences, and the maximum temperature of the nozzle during the printing process is above 300 ° C, and There is a large shear stress, and the instantaneous high temperature, high voltage or instantaneous strong electrostatic field during the cell printing process has caused damage to the cells

Method used

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  • Preparation method and application of cell-loading three-dimensional support
  • Preparation method and application of cell-loading three-dimensional support
  • Preparation method and application of cell-loading three-dimensional support

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A method for preparing a cell-loaded three-dimensional scaffold based on cell printing output technology, comprising the following steps:

[0035] The first step is to prepare cell-loaded microspheres, mix chondrocytes with 1 wt % sodium alginate aqueous solution sterilized by high temperature intermittently, and the concentration of chondrocytes in the mixture is 2.2×10 5 / mL, according to the injection conditions of pressure 120 kPa and nozzle diameter 730 μm, the mixed solution was sprayed to a concentration of 2 wt% crosslinking agent CaCl 2 solution, let stand for 10min, remove CaCl2 solution, rinsed 3 times with PBS solution, and 3 times with DMEN cell culture solution to obtain cell-loaded microspheres for later use.

[0036] In the second step, the matrix material was prepared, and a recombinant collagen solution with a concentration of 7wt% and a sodium alginate solution with a concentration of 7wt% were respectively prepared, mixed at a mass ratio of 1:1, and ...

Embodiment 2

[0043] A method for preparing a cell-loaded three-dimensional scaffold based on cell printing output technology, comprising the following steps:

[0044] The first step is to prepare cell-loaded microspheres, mix bone marrow mesenchymal stem cells with 2.5 wt % sodium alginate aqueous solution which is intermittently sterilized at high temperature, and the concentration of chondrocytes in the mixture is 5×10 5 / mL, according to the injection conditions of pressure 150 kPa and nozzle diameter 500 μm, the mixed solution was sprayed to a concentration of 5 wt% crosslinking agent CaCl 2 solution, let stand for 10 min, remove CaCl 2 solution, rinsed 3 times with PBS solution, and 3 times with DMEN cell culture solution to obtain cell-loaded microspheres for later use.

[0045] The second step is to prepare the matrix material, respectively prepare a recombinant collagen solution with a concentration of 3 wt% and a sodium alginate solution with a concentration of 5 wt%, mix them at...

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Abstract

The invention provides a preparation method of a cell-loading three-dimensional support. The preparation method of the cell-loading three-dimensional support is characterized by comprising the following steps: uniformly mixing autologous cells with a high temperature intermittently sterilized hydrogel solution to obtain mixed liquor, injecting the mixed liquor into a crosslinking agent solution, standing, then removing the crosslinking agent solution, and rinsing, so that cell-loading microspheres are obtained; mixing the cell-loading microspheres with a matrix material to obtain cell blend; and extruding the cell blend onto a three-dimensional micro-positioning platform carrying a sterile glass base plate by adopting a biological printer, and overlaying layer by layer, so that the cell-loading three-dimensional support is formed. The preparation method of the cell-loading three-dimensional support has the advantages that damage on cellular activity and bioactivity in a cell printing process is reduced, and high-activity cell printing is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of cell printing and biomaterials, and is particularly suitable for the preparation of scaffold materials for biomedical tissue engineering, specifically a method for preparing a three-dimensional scaffold loaded with cells. Background technique [0002] The combination of life science, information science and manufacturing science is an important trend in the development of science and technology in the 21st century. Cell 3D printing technology based on advanced manufacturing theory and tissue engineering theory has expanded new theoretical and technical space for tissue engineering, pathological model construction, drug screening and detection, cell biology research and other fields. [0003] Compared with traditional tissue engineering methods, the advantages of cell printing mainly include: (1) Simultaneous construction of a biologically active two-dimensional or three-dimensional "cell / material" system;...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/24A61L27/22A61L27/20
Inventor 王红张楠
Owner 深圳尤尼智康医疗科技有限公司
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