Method for extracting trypsin inhibitor from bamboo shoots

A technology of trypsin inhibition and bamboo shoots, which is applied in the field of protein preparation, can solve the problems of low economic benefits of enterprises, low price of raw materials of fresh bamboo shoots, waste of resources and the environment, and achieve the effects of promoting technological upgrading, improving resource utilization efficiency, and improving economic benefits

Inactive Publication Date: 2015-04-29
JIANGXI ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in recent years, due to the saturation of the market for traditional bamboo shoots and fierce competition in the market, the economic benefits of related enterprises have been low, the raw material prices of fresh bamboo shoots have been low, and farmers have inc

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  • Method for extracting trypsin inhibitor from bamboo shoots

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Embodiment 1

[0014] The bamboo shoots were peeled and washed with water, cut into small pieces of about 3×5cm, and then the bamboo shoots were added to the water according to the ratio of bamboo shoots: water mass to volume 1g: 1ml, ground with a mixer, and then reacted at 60 ° C for 3 minutes, filtered, Remove the residue to take the filtrate, slowly add 600g / L ammonium sulfate solution to the filtrate while stirring, until the solution is clear, leave it at 3°C ​​for 8 hours, then centrifuge the solution overnight, remove the supernatant, and redissolve the precipitate In water, the protein concentration was adjusted to 1% by mass to obtain crudely extracted bamboo shoots trypsin inhibitor. Take 5ml of sample and load it on Sephadex G25 column, elute with ultrapure water, concentrate the eluted fraction to 5ml, and then pass through Sephadex G50 column (2×93.5cm) and elute with ultrapure water , the eluted fraction was concentrated, and then passed through diethylenediamine ethyl cellulo...

Embodiment 2

[0016] The bamboo shoots are peeled and washed with water, cut into small pieces of about 3 × 5cm, and then the bamboo shoots are added to the water according to the ratio of bamboo shoots: water mass to volume: 1g: 5ml, ground with a mixer, and then reacted at 90 ° C for 30 minutes, filtered, Remove the residue to take the filtrate, slowly add 750g / L ammonium sulfate solution to the filtrate while stirring, until the solution is clear, leave it at 7°C for 12 hours, then centrifuge the overnight solution, remove the supernatant, and redissolve the precipitate In water, the protein concentration was adjusted to 1% by mass to obtain crudely extracted bamboo shoots trypsin inhibitor. Take 5ml of sample and load it on Sephadex G25 column, elute with ultrapure water, concentrate the eluted fraction to 5ml, and then pass through Sephadex G50 column (2×93.5cm) and elute with ultrapure water , the eluted fraction was concentrated, and then passed through diethylenediamine ethyl cellul...

Embodiment 3

[0018] The bamboo shoots are peeled and washed with water, cut into small pieces of about 3 × 5cm, and then the bamboo shoots are added to the water according to the ratio of bamboo shoots: water mass to volume: 1g: 3ml, ground with a mixer, and then reacted at 70 ° C for 15 minutes, filtered, Remove the residue to take the filtrate, slowly add 670g / L ammonium sulfate solution to the filtrate while stirring, until the solution is clear, leave it at 5°C for 10 hours, then centrifuge the overnight solution, remove the supernatant, and redissolve the precipitate In water, the protein concentration was adjusted to 1% by mass to obtain crudely extracted bamboo shoots trypsin inhibitor. Take 5ml of sample and load it on Sephadex G25 column, elute with ultrapure water, concentrate the eluted fraction to 5ml, and then pass through Sephadex G50 column (2×93.5cm) and elute with ultrapure water , the eluted fraction was concentrated, and then passed through diethylenediamine ethyl cellul...

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Abstract

The invention discloses a method for extracting a trypsin inhibitor from bamboo shoots. The method comprises the following steps: peeling the bamboo shoots, cleaning with water and slicing; adding the sliced bamboo shoots into water according to a mass volume ratio 1g:(1-5)ml of the bamboo shoots to water; grinding and reacting for 3-30min at 60-90 DEG C; filtering, removing the residue and taking the filtrate; adding a 600-750g/L ammonium sulfate solution into the filtrate while stirring until the solution is clear; standing for 8-12 hours at 3-7 DEG C; centrifuging, removing the supernate and dissolving the precipitate in water; treating through a sephadex G25 column and eluting with water; concentrating the elution fraction and treating through a sephadex G50 column and eluting with water; treating the elution fraction with diethylaminoethylcellulose-sephadex A50; performing gradient elution with a 0.1-0.3g/L NaCl liquid; and collecting 0.12-0.15g/L gradient elution fraction to obtain the trypsin inhibitor. The method disclosed by the invention can be used for developing bamboo shoot products with high added value, the economic benefit of the bamboo shoot processing industry is increased, the resource utilization efficiency is improved, and the development of bamboo industry and the prosperity of rural economy are promoted.

Description

Technical field: [0001] The invention belongs to the field of protein preparation, in particular to a method for extracting trypsin inhibitor from bamboo shoots. Background technique: [0002] my country has very rich bamboo resources, mainly distributed in Fujian, Jiangxi, Zhejiang and other provinces. Bamboo shoots are swollen buds and young stems formed by germination and differentiation of buds on bamboo whip or stalk base. It is a high-protein and low-fat forest vegetable. With the promotion of high-yield technology in bamboo shoot forests and bamboo shoot dual-purpose forests, the output of fresh bamboo shoots in my country has been increasing year by year. processing. However, in recent years, the market for traditional bamboo shoot products has become saturated and the market competition is fierce, resulting in low economic benefits of related enterprises, low raw material prices of fresh bamboo shoots, and increased production but not income of farmers. In additio...

Claims

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Application Information

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IPC IPC(8): C07K14/81C07K1/16
CPCC07K14/8117
Inventor 余能富王玉王小东贺磊黄慧
Owner JIANGXI ACAD OF FORESTRY
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