Preparation method of mesoporous apatite nano-drug carrier with pH responsiveness and cellular targeting property for hepatoma cell

A nano-drug carrier and mesoporous apatite technology, which is applied in the field of biomedical materials, can solve the problems of inability to specifically identify target cells, increase drug toxicity and side effects, and low drug delivery ability, thereby reducing drug intake and cost. Inexpensive, practical effect

Active Publication Date: 2015-05-06
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the problem that the carrier prepared by the existing method cannot specifically recognize the target cells, and most of the drug molecules are distributed in normal tissues and taken up by normal cells, while the drug distribution in diseased tissues is less, resulting in low drug delivery ability. Increased the technical problem of toxic and side effects of drugs in the body, thus providing a method for preparing a mesoporous apatite nano-drug carrier that is pH-responsive and cell-targeted to liver cancer cells

Method used

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  • Preparation method of mesoporous apatite nano-drug carrier with pH responsiveness and cellular targeting property for hepatoma cell
  • Preparation method of mesoporous apatite nano-drug carrier with pH responsiveness and cellular targeting property for hepatoma cell
  • Preparation method of mesoporous apatite nano-drug carrier with pH responsiveness and cellular targeting property for hepatoma cell

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specific Embodiment approach 1

[0033] Embodiment 1: A method for preparing a mesoporous apatite nano-drug carrier that is pH-responsive and cell-targeted to liver cancer cells in this embodiment is carried out in the following steps:

[0034] 1. Preparation of the template agent: Dissolve the template agent F127 and calcium pantothenate monohydrate in deionized water, and stir at a stirring speed of 250r / min to 400r / min until a clear emulsion is obtained to obtain the F127-calcium pantothenate mixed solution. Promptly obtain templating agent;

[0035] The mass ratio of template agent F127 described in step 1 to calcium pantothenate monohydrate is 1: (2.5-8); the mass ratio of F127 described in step 1 to deionized water is 1: (15-67);

[0036] 2. Preparation of mesoporous hydroxyapatite nanoparticles: K 2 HPO 4 ·3H 2 O is dissolved in deionized water to give K 2 HPO 4 solution, followed by NH 3 ·H 2 O Mediation K 2 HPO 4 The pH value of the solution is adjusted to a pH value of 10-14, and then the p...

specific Embodiment approach 2

[0055] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the mass ratio of template agent F127 and calcium pantothenate monohydrate described in step 1 is 2.0:10.12. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0056] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the volume ratio of the template agent F127 described in step 1 to deionized water is 2.0 g: 80 mL. Other steps and parameters are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a preparation method of a mesoporous apatite nano-drug carrier with pH responsiveness and cellular targeting property for a hepatoma cell, belonging to the field of biomedical materials. The invention aims at solving the technical problems that the drug delivery capacity is relatively low and the in-vivo toxic and side effects of a drug are enhanced because a carrier prepared by using the existing method cannot specifically recognize a target cell, most of drug molecules are distributed in normal tissues and are absorbed by normal cells, but the dosage of the drug distributed in pathological tissues is relatively small. The preparation method comprises the steps of firstly, preparing a template agent; secondly, preparing a mesoporous hydroxylapatite nanoparticle; thirdly, aminating mesoporous hydroxylapatite; fourthly, borating mesoporous apatite; fifthly, preparing lactobionic acid functionalized bovine serum albumin; and sixthly, preparing the mesoporous apatite nano-drug carrier with pH responsiveness and cellular targeting property. The mesoporous apatite nano-drug carrier prepared by using the preparation method can be used for recognizing hepatoma cells in a targeting way, so that the uptake of the normal tissues to the drug is reduced, and the in-vivo toxic and side effects of the drug are reduced.

Description

Technical field: [0001] The invention belongs to the field of biomedical materials, and in particular relates to a preparation method of a mesoporous apatite nano drug carrier with pH responsiveness and cell targeting. Background technique: [0002] With the increasing incidence of malignant tumors year by year, the intelligent responsive drug delivery system has attracted more and more attention. The intelligent responsive system is that after arriving at the target affected area, the drug carrier generates an intelligent response to changes in the surrounding environment or external stimuli to achieve the purpose of controlled drug release. So far, a series of responsive release drug carriers have been constructed, such as temperature response, light response, and ultrasonic response. This type of system can exist stably in the circulation in the body. However, these responsive releases of drugs come from external additional stimuli rather than changes in the physiologica...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K9/19A61K47/42A61K47/04A61K31/704A61K49/00A61P35/00A61K47/52A61K47/69
Inventor 黄玉东李大龙贺金梅李纪伟黄鑫程玮璐吴亚东
Owner HARBIN INST OF TECH
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