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Culture media for improving polysaccharide content of dendrobium officinale plants and culture method of dendrobium officinale plants

A cultivation substrate, Dendrobium officinale technology, applied in the directions of planting substrate, botanical equipment and methods, cultivation, etc., can solve the problems of sonic stimulation, unfavorable industrial production and application, complicated operation, etc., and achieve the effect of increasing the content of polysaccharides

Active Publication Date: 2015-05-13
贵州易通实业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent with the authorized notification number CN102172152B uses light, temperature, fertilizer spraying, water irrigation, and sound wave stimulation at the same time, and combines the method of bud control when the buds just germinate and the method of manually removing the buds that have already bloomed. The results show that this method can Increase the content of polysaccharides in Dendrobium officinale, but this method is complicated to operate and is not conducive to industrial production and application

Method used

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  • Culture media for improving polysaccharide content of dendrobium officinale plants and culture method of dendrobium officinale plants
  • Culture media for improving polysaccharide content of dendrobium officinale plants and culture method of dendrobium officinale plants
  • Culture media for improving polysaccharide content of dendrobium officinale plants and culture method of dendrobium officinale plants

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0045] Experimental Example 1 Effects of Cultivation Substrate on Dendrobium officinale Plant Growth State and Polysaccharide Content

[0046] 1. Preparation of test-tube plantlets of Dendrobium officinale

[0047] (1) The explant disinfection material comes from wild Dendrobium officinale in Guizhou. Select a healthy plant with a height of 3-5cm, cut off the stem segment with a blade, first scrub the whole young stem with leaves and leaf sheaths with 70% ethanol degreasing cotton wool, then peel off the lower leaves and leaf sheaths, leaving the wrapped stems For 1-2 pointed leaves, soak in 1g / L mercury liter (plus 2 drops of Tween 20) for 8 minutes, in 100g / L NaCl0 solution for 20 minutes, wash 4-5 times with distilled water, squeeze out the shoot tip with the back of a knife to inoculate.

[0048] (2) Medium and culture conditions: Adventitious bud induction medium is 1 / 2MS+3.0mg / L6-BA+0.75mg / L NAA+100g / L coconut milk+7.Og / L agar+25g / L sucrose, Subculture once every 50 da...

experiment example 2

[0079] Experimental Example 2 The humidity control of the cultivation medium and the influence of potassium fertilizer on the polysaccharide content of Dendrobium officinale plants

[0080] 1. Preparation of test-tube plantlets of Dendrobium officinale

[0081] The obtaining method of Dendrobium officinale test-tube plantlet is the same as in Experimental Example 1.

[0082] 2. Transplanting test-tube seedlings of Dendrobium officinale

[0083] Select the test-tube seedlings of Dendrobium candidum with a height of 3-4cm and 2-5 roots to be transplanted into the following cultivation substrates for further cultivation, and transplant 400 strains of Dendrobium candidum seedlings in each cultivation substrate. When transplanting the test-tube seedlings, first remove the medium at the root, wash off the remaining medium with clean water, and then transplant it into a cultivation tray with a plant spacing of 5 cm.

[0084] The composition of table 4 cultivation medium

[0085] ...

Embodiment 1

[0103] Select the test-tube seedlings of Dendrobium officinale with a height of 3 to 4 cm and 2 to 5 roots to be transplanted in the following cultivation substrates and continue to cultivate. It is a pine wood block with a thickness of 4.0 cm; 100 Dendrobium officinale seedlings are transplanted. When transplanting the test-tube seedlings, first remove the medium at the root, wash off the remaining medium with clean water, and then transplant it into a cultivation tray with a plant spacing of 5 cm.

[0104] After the Dendrobium candidum test-tube plantlets are transplanted to the above-mentioned cultivation substrate, they should be managed as normal before the middle and early August, and watered when the moisture content of the cultivation substrate is lower than 80%, and when watering, they should be poured until the lower layer of the cultivation substrate drips. After mid-to-late August, water only when the water content of the cultivation substrate is lower than 50%, an...

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Abstract

The invention discloses culture media for improving polysaccharide content of dendrobium officinale plants and a culture method of the dendrobium officinale plants. An upper layer of the culture media for improving the polysaccharide content of the dendrobium officinale plants is made of a mixed medium of wood flour and SiO2 powder, a lower layer of the culture media is made of wood blocks, and the grain diameter of the wood blocks ranges from 0.5 cm to 4.0 cm. According to the culture method of the dendrobium officinale plants, the dendrobium officinale plants are planted in the culture media, before the middle early days of August, when the water content of the culture media is reduced to 80 percent, the culture media are watered until water drips from the lower layer of the culture media, after the middle of August, when the water content of the culture media is reduced to 50 percent, watering is carried out, only the upper layer of the culture media is watered wet, potash fertilizer is increased and applied, from the middle of August, the potash fertilizer is applied once every month until harvesting is carried out, and 3 kg of potassium chloride is applied at a time per mu.

Description

technical field [0001] The invention relates to plant cultivation and a cultivation method thereof, in particular to a cultivation substrate for increasing the polysaccharide content of a dendrobium candidum plant and a cultivation method for a dendrobium candidum plant. Background technique [0002] Dendrobium officinale Kimura et Migo is a traditional Chinese medicinal material in my country. It is a rare medicinal plant in the genus Dendrobium of Orchidaceae. It is mainly distributed in Guizhou, Yunnan, Guangxi, Jiangxi, Anhui and Hunan. According to the "Compendium of Materia Medica", Dendrobium candidum has the effects of "curing mainly injuries, nourishing five viscera and fatigue, strengthening yin and nourishing essence, nourishing internal deficiency, calming stomach qi, growing muscles, improving intelligence and eliminating convulsions, lightening body and prolonging life". According to the 2010 edition of "The Pharmacopoeia of the People's Republic of China", Den...

Claims

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Application Information

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IPC IPC(8): A01G31/00C05G3/00
CPCA01G24/00A01G24/23A01G31/00
Inventor 倪石寿
Owner 贵州易通实业股份有限公司