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Method for preparing biological beautifying facial mask containing adipose derived stem cells and product thereof

A technology of fat stem cells and autologous organisms, which is applied in the preparation of bio-cosmetic facial masks containing autologous adipose-derived stem cells and its products, which can solve the problems of limited moisturizing, whitening and oil control, and limited bio-cosmetic facial masks, and achieve the best cosmetic effect.

Inactive Publication Date: 2015-05-20
广州赛琅生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, the effects of bio-cosmetic masks on the market on skin beauty are very limited, limited to moisturizing, whitening and oil control.

Method used

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  • Method for preparing biological beautifying facial mask containing adipose derived stem cells and product thereof
  • Method for preparing biological beautifying facial mask containing adipose derived stem cells and product thereof
  • Method for preparing biological beautifying facial mask containing adipose derived stem cells and product thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 Preparation of human autologous adipose stem cells

[0051] Beauty volunteers obtained 100ml of adipose tissue fluid from abdominal liposuction (with whom they signed an informed consent form), and washed it with 1×PBS (pH value 7.4) for 5 times to remove the drugs, oil and blood cells used in liposuction as much as possible. Digest with 0.1% (mass volume ratio) type I collagenase (Sigma, USA) at 37° C. for 60 minutes, and repeatedly pipette several times with a 1 ml pipette at intervals of 10 minutes. After digestion, centrifuge at 1000 rpm for 10 minutes, absorb and discard the upper layer of fat, add 1×PBS (pH value is 7.4) and mix well, and filter with a 200-mesh cell sieve (BD Company, USA); the obtained cell suspension is 1000 rpm Centrifuge for 10 minutes, wash the cell pellet once with 1×PBS (pH value 7.4), resuspend in α-MEM culture solution (Invitrogen, USA) containing 10% fetal bovine serum by volume fraction, and stain and count Nucleated cells ar...

Embodiment 2

[0054] Example 2 Flow cytometric detection of autologous adipose-derived stem cells

[0055] Take the 3 × 10 prepared in Example 1 6 Adipose-derived stem cells were divided into three groups. The first group was added to 20 μL FITC-labeled mouse anti-human CD29 monoclonal antibody, 20 μL PE-labeled mouse anti-human CD44 monoclonal antibody and 20 μL Percp-labeled mouse anti-human CD34 monoclonal antibody; the second group was added to There are 20 μL FITC-labeled mouse anti-human CD45 monoclonal antibody, 20 μL PE-labeled mouse anti-human CD105 monoclonal antibody and 20 μL Percp-labeled mouse anti-human CD14 monoclonal antibody; the third group is the isotype control, added to 20 μL FITC-labeled mouse IgG1, 20 μL PE Label mouse IgG1 and 20 μL PerCP-labeled mouse IgG1; place in a refrigerator at 4°C for 30 minutes for staining, then wash 3 times with 1 mL of 1×PBS (pH 7.4), and finally resuspend the washed cells with 0.5 mL of 1×PBS , The resulting washed cells were detected wi...

preparation example 3

[0056] Preparation Example 3 Preparation of Human Autologous Plasma

[0057] Use 10ml EDTA anticoagulant blood collection tubes (BD Company, USA) to extract 100ml anticoagulant meridian blood, and centrifuge 10 10ml anticoagulant tubes at 1000 rpm for 10 minutes to obtain 65ml plasma; centrifuge again at 3000 rpm for 10 minutes , obtain 10-80ml of platelet-rich plasma, and store it in the refrigerator at 4°C for later use.

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Abstract

The invention provides a method for preparing a biological beautifying facial mask containing adipose derived stem cells. The method comprises the following steps: culturing and acquiring adipose-derived stem cells from autogenous fat, and acquiring platelet enriched plasma from autogenous peripheral blood; adding agarose into normal saline, heating and dissolving, and adding the platelet enriched plasma when the liquid temperature is reduced to about 40 DEG C; uniformly mixing, and adding adipose derived stem cells to prepare the biological beautifying facial mask. Furthermore, nutritional factors can be added to prepare a personalized biological beautifying facial mask product. The facial mask can be used for removing skin wrinkles and acne marks, repairing scars, lightening spots, resisting oxidation and the like.

Description

technical field [0001] The invention relates to a method for preparing a biological cosmetic facial mask containing autologous fat stem cells and a biological cosmetic facial mask product containing autologous fat stem cells obtained by the method. In particular, the present invention relates to the use of adipose stem cells derived from autologous fat and platelet-rich plasma derived from autologous peripheral blood, which are added to agarose to form a semi-gel mask, and nutritional factors can also be added at the same time, and obtained by the method Personalized bio-beauty mask products. The biological cosmetic facial mask of the invention can be used for medical cosmetology, and has the effects of wrinkle removal, acne scar and scar repair, spot removal and anti-oxidation on the skin. Background technique [0002] At present, various facial mask products have been widely used in the market, most of which are made of non-woven fabrics, crystal cloth and biological fibe...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/73A61K8/02A61Q19/08A61Q19/00A61P17/02A61P17/10A61P17/00
Inventor 陈镇洲寇蕊刘兵
Owner 广州赛琅生物技术有限公司
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