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Efficient extraction method of total coptis alkaloids

A technology of total biological alkaloids and extraction methods, which is applied in the direction of drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve the problems of low purity and yield, and achieve the effects of increasing final yield, reducing production cost, and low energy consumption

Inactive Publication Date: 2015-05-27
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] The technical problem to be solved in the present invention is the problem that the purity and yield are not high in the extraction of the total alkaloids of Coptis chinensis

Method used

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  • Efficient extraction method of total coptis alkaloids
  • Efficient extraction method of total coptis alkaloids
  • Efficient extraction method of total coptis alkaloids

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1 Comparative Study Results of Coptidis Rhizoma Grinding Particle Size and Extraction Rate

[0049] ①, Coptis chinensis crushed to 150 mesh

[0050] Grind 100g of Coptis Rhizoma to 150 mesh; add 0.5 times volume of perlite to Coptidis Rhizoma fine powder, then add 3 times volume concentration of 1% sulfuric acid aqueous solution (V / V) and stir evenly; the prepared solution is transferred to percolation In the cylinder, after soaking for 6 hours at 15°C, percolation begins, and the percolation rate is 0.1 times volume / hour; 8 times the volume of percolation liquid is collected to obtain 800 ml. Sampling was carried out to analyze the content of total alkaloids in Coptis chinensis, and the extraction rate was calculated.

[0051] The percolation solution is neutralized with calcium hydroxide until the pH of the solution is 5, and then filtered; the residue is washed once with water of 1 times the amount of the residue, and the alkaloids taken away by the residue ...

Embodiment 2

[0065] Example 2 Contrastive Experiment of Coptis Rhizoma Grinding Particle Size and Filtration (Percolation) Speed

[0066] ①, Coptis chinensis crushed to 150 mesh

[0067] Grind 100 g of Coptis Rhizoma to 150 mesh; add 3 times volume concentration of 1% sulfuric acid aqueous solution (V / V) to Coptis Rhizoma fine powder and stir evenly; transfer the prepared solution into the percolation cylinder, start percolation, and measure percolation Speed, the results are shown in Table 2. Eight times the volume of the percolate was collected, yielding 800 ml. Sampling was carried out to analyze the content of total alkaloids in Coptis chinensis, and the extraction rate was calculated.

[0068] ②, Coptis chinensis crushed to 100 mesh

[0069] Except that the particle size of Coptis rhizome raw materials is different, the other operations are the same as ①.

[0070] ③, Coptis chinensis crushed to 50 mesh

[0071] Except that the particle size of Coptis rhizome raw materials is diff...

Embodiment 3

[0079] Example 3 Comparative experimental research on the influence of additives on the percolation (filtration) speed of 150 mesh Coptidis fine powder

[0080] ①, 150 mesh Coptis fine powder

[0081] Pulverize 100g into 150 mesh Coptis rhizome fine powder, add 3 times volume concentration and be 1% sulfuric acid aqueous solution (V / V) and stir evenly; See Table 3. Eight times the volume of the percolate was collected, yielding 800 ml. Sampling was carried out to analyze the content of total alkaloids in Coptis chinensis, and the extraction rate was calculated.

[0082] ②, 150 mesh Coptis fine powder + 1% Coptis fine powder weight silica gel (or perlite)

[0083] Except adding 1% silica gel (or perlite), the other operations are the same as ①.

[0084] ③, 150 mesh Coptis fine powder + 10% silica gel (or perlite)

[0085] Except adding 10% silica gel (or perlite), the other operations are the same as ①.

[0086] ④, 150 mesh Coptis fine powder + 100% silica gel (or perlite) ...

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Abstract

The invention belongs to the technical field of extraction of active ingredients of traditional Chinese medicines, and particularly relates to an efficient extraction method of total coptis alkaloids. The efficient extraction method aims at solving the technical problems that the purity and the yield of the total coptis alkaloids extracted in the prior art are not high. The efficient extraction method of the total coptis alkaloids is characterized in that the efficient extraction method comprises the following steps of 1) smashing raw materials, 2) pretreating the raw materials, 3) percolating, 4) neutralizing percolation liquid, 5) concentrating, 6) salting out, 7) filtering and washing, 8) treating total alkaloid mother liquid, and 9) drying. The efficient extraction method is simple to operate and low in energy consumption; the cost of industrial production is lowered greatly; the extraction yield of the total coptis alkaloids reaches above 98%; and main active ingredients of the total coptis alkaloids are berberine hydrochloride, namely berberin, coptisine, epiberberine, palmatine, jateorhizine, etc.

Description

technical field [0001] The invention belongs to the technical field of extraction of active ingredients of traditional Chinese medicines, and in particular relates to an efficient extraction method of total biological alkaloids of Coptidis Rhizoma. Background technique [0002] Coptis chinensis was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Coptis chinensis is bitter in taste, cold in nature, and has the functions of purging fire, detoxifying, clearing away heat, and drying dampness. For dysphoria, confusion, upset and insomnia, hot and humid fullness, vomiting, abdominal pain and diarrhea, conjunctival congestion and swelling, sore tongue, eczema, burns, vomiting blood, epistaxis, etc. The results of modern pharmaceutical research show that the main active ingredient of Coptis chinensis is the total alkaloids of Coptis chinensis, mainly including berberine (berberine), palmatine, coptisine, epiberberine and jatrorrhizine, etc. ("Chinese Pharmac...

Claims

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Application Information

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IPC IPC(8): A61K36/718A61K36/758A61K36/29A61P3/06A61P3/10A61P3/04A61P9/00
Inventor 李学刚叶小利
Owner SOUTHWEST UNIVERSITY
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