Efficient extraction method of total coptis alkaloids

A technology of total biological alkaloids and extraction methods, which is applied in the direction of drug combinations, pharmaceutical formulas, plant raw materials, etc., can solve the problems of low purity and yield, and achieve the effects of increasing final yield, reducing production cost, and low energy consumption

Inactive Publication Date: 2015-05-27
SOUTHWEST UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] The technical problem to be solved in the present invention is the problem that the

Method used

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  • Efficient extraction method of total coptis alkaloids
  • Efficient extraction method of total coptis alkaloids
  • Efficient extraction method of total coptis alkaloids

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0048] Embodiment 1 The comparative study results of the relationship between the pulverized particle size and extraction rate of Coptis chinensis

[0049] ①, Coptis is crushed to 150 mesh

[0050] Pulverize 100 g of Coptis chinensis to 150 mesh; add 0.5 times the volume of perlite to Coptis chinensis fine powder, then add 3 times the volume concentration of 1% sulfuric acid aqueous solution (V / V) and stir evenly; After soaking at 15°C for 6 hours, the percolation was started, and the percolation speed was 0.1 times the volume / hour; 8 times the volume of the percolation solution was collected to obtain 800 ml. Samples were taken to analyze the content of total alkaloids in Coptis chinensis, and the extraction rate was calculated.

[0051] The leachate is neutralized with calcium hydroxide to pH=5 of the solution, and filtered; the residue is washed once with 1 times the amount of water of the residue, and the alkaloids taken away by the residue are recovered to obtain a washi...

Example Embodiment

[0065] The comparative experiment of embodiment 2 Coptis chinensis crushing particle size and filtration (percolation) speed

[0066] ①, Coptis is crushed to 150 mesh

[0067] Pulverize 100 g of Coptis chinensis to 150 mesh; add 3 times the volume concentration of 1% sulfuric acid aqueous solution (V / V) to Coptis chinensis fine powder and stir evenly; transfer the prepared solution into the percolation cylinder, start percolation, and measure the percolation speed, and the results are shown in Table 2. Eight volumes of the permeate were collected, yielding 800 mL. Samples were taken to analyze the content of total alkaloids in Coptis chinensis, and the extraction rate was calculated.

[0068] ②, Coptis is crushed to 100 mesh

[0069] Except for the fact that the pulverized particle size of the raw materials of Coptis chinensis is different, the rest of the operations are the same as ①.

[0070] ③, Coptidis pulverized to 50 mesh

[0071] Except for the fact that the pulver...

Example Embodiment

[0079] Example 3 Comparative experimental study on the effect of additives on the percolation (filtration) speed of 150 mesh Coptidis fine powder

[0080] ①, 150 mesh Coptis chinensis powder

[0081] Pulverize 100 g into 150 mesh Coptis chinensis fine powder, add 3 times the volume concentration of 1% sulfuric acid aqueous solution (V / V) and stir evenly; transfer the prepared solution into the percolation cylinder, start percolation, measure the percolation speed, the result See Table 3. Eight volumes of the permeate were collected, yielding 800 mL. Samples were taken to analyze the content of total alkaloids in Coptis chinensis, and the extraction rate was calculated.

[0082] ②, 150 mesh coptis fine powder + 1% coptis fine powder weight of silica gel (or perlite)

[0083] Except adding 1% silica gel (or perlite), other operations are the same as ①.

[0084] ③, 150 mesh coptis fine powder + 10% silica gel (or perlite)

[0085] Except adding 10% silica gel (or perlite), oth...

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Abstract

The invention belongs to the technical field of extraction of active ingredients of traditional Chinese medicines, and particularly relates to an efficient extraction method of total coptis alkaloids. The efficient extraction method aims at solving the technical problems that the purity and the yield of the total coptis alkaloids extracted in the prior art are not high. The efficient extraction method of the total coptis alkaloids is characterized in that the efficient extraction method comprises the following steps of 1) smashing raw materials, 2) pretreating the raw materials, 3) percolating, 4) neutralizing percolation liquid, 5) concentrating, 6) salting out, 7) filtering and washing, 8) treating total alkaloid mother liquid, and 9) drying. The efficient extraction method is simple to operate and low in energy consumption; the cost of industrial production is lowered greatly; the extraction yield of the total coptis alkaloids reaches above 98%; and main active ingredients of the total coptis alkaloids are berberine hydrochloride, namely berberin, coptisine, epiberberine, palmatine, jateorhizine, etc.

Description

technical field [0001] The invention belongs to the technical field of extraction of active ingredients of traditional Chinese medicines, and in particular relates to an efficient extraction method of total biological alkaloids of Coptidis Rhizoma. Background technique [0002] Coptis chinensis was first recorded in "Shen Nong's Materia Medica" and listed as top grade. Coptis chinensis is bitter in taste, cold in nature, and has the functions of purging fire, detoxifying, clearing away heat, and drying dampness. For dysphoria, confusion, upset and insomnia, hot and humid fullness, vomiting, abdominal pain and diarrhea, conjunctival congestion and swelling, sore tongue, eczema, burns, vomiting blood, epistaxis, etc. The results of modern pharmaceutical research show that the main active ingredient of Coptis chinensis is the total alkaloids of Coptis chinensis, mainly including berberine (berberine), palmatine, coptisine, epiberberine and jatrorrhizine, etc. ("Chinese Pharmac...

Claims

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Application Information

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IPC IPC(8): A61K36/718A61K36/758A61K36/29A61P3/06A61P3/10A61P3/04A61P9/00
Inventor 李学刚叶小利
Owner SOUTHWEST UNIVERSITY
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