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A Desulfurization Bacteria Efficiently Degrading DBTs and Its Application in Desulfurization

A technology for removing sulfur elements, applied in the direction of microorganism-based methods, bacteria, biochemical equipment and methods, etc., can solve the problems of poor tolerance to organic solvents, low desulfurization efficiency, etc., and achieve high desulfurization ability

Active Publication Date: 2018-01-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many bottlenecks in the actual production of this technology. One of the biggest problems is that the desulfurization efficiency of the desulfurization strains isolated in the world is not high, and the tolerance to organic solvents is poor.

Method used

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  • A Desulfurization Bacteria Efficiently Degrading DBTs and Its Application in Desulfurization
  • A Desulfurization Bacteria Efficiently Degrading DBTs and Its Application in Desulfurization
  • A Desulfurization Bacteria Efficiently Degrading DBTs and Its Application in Desulfurization

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Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1: strain screening

[0024] Dissolve about 2g of crude oil-contaminated soil in Karamay Oilfield, Xinjiang, into 100ml of BSM-DBT culture solution, enrich and cultivate for 4 days, repeat 3-4 times, then take the culture solution and extract it with ethyl acetate for high-pressure liquid phase analysis Its metabolites, for the culture solution containing dihydroxybiphenyl, use the line drawing method and coating method to separate in the BSM-DBT solid selection medium, after 3 days, the colony is formed, pick a single colony and put it into the BSM-DBT liquid Cultivate in the culture medium, and measure the metabolites with the above method after four days of cultivation, and repeat the purification for 4 times to obtain the pure strains of the strains that can metabolize dibenzothiophene and convert to dihydroxybiphenyl.

Embodiment 2

[0025] Embodiment 2: bacterial strain identification

[0026] The entire genome of the pure culture was extracted using a bacterial whole genome rapid extraction kit, and PCR was performed with 16S rDNA universal primers. The strain was identified as Gordonia sp. and named Gordoniasp.JDZX5.

Embodiment 3

[0027] Embodiment 3: Physiological and biochemical studies of bacterial strains

[0028] The morphology of the above-mentioned JDZX5 was observed by transmission electron microscope as follows: figure 1 . It can be seen from the figure that the bacterium is short rod-shaped and has a capsule. The optimum growth temperature of the strain is 30°C, and it can grow well at 28-37°C. Take the initial pH 3.0, 4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0 and 11.0 to culture at the optimum temperature, and determine the optimum growth pH 7.0. The bacterium has stronger tolerance to alkali than acid, and can grow well at pH 6-10. When the pH is below 4.0, the bacterium hardly grows, and when the pH is 11, it can still grow slowly.

[0029] Through the physiological and biochemical experiments such as Gram staining, capsule staining, oxidase test and contact enzyme test, it is determined that the bacteria is Gram-positive, has no flagella, has a capsule, is negative for oxidase, and is positive...

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Abstract

The invention discloses a desulfurization bacterium for efficiently degrading DBT and its application in desulfurization, belonging to the technical field of biological desulfurization. The Gordonia sp. JDZX5 of the present invention has a preservation number: CCTCC NO: M 2014650. The strain has the ability to degrade 4,6-dimethyldibenzothiophene, dibenzothiophene, benzothiophene, and thiophene, indicating that the strain has a wide substrate utilization ability and strong tolerance to organic solvents . When using the growing cells and resting cells of the bacteria in single-water and oil-water two-phase systems to desulfurize organic sulfur compounds modeled on dibenzothiophene, it was found that the bacteria can rapidly desulfurize in the oil-water two-phase system. growth, indicating that the strain has strong tolerance to organic solvents, and it was found that the strain had higher desulfurization efficiency in the oil-water two-phase system. The bacterium is suitable for the desulfurization of industrial fuel oil and is expected to be widely used in the desulfurization of industrial fuel oil.

Description

technical field [0001] The invention relates to a desulfurization bacterium for efficiently degrading DBT and its application in desulfurization, belonging to the technical field of biological desulfurization. Background technique [0002] While the development and utilization of petroleum promotes rapid economic development, it also brings a series of problems, the most prominent of which is the sulfur oxide released by its combustion, which will have a bad impact on human health, the environment and the national economy. For example, acid rain and acid mist formed by sulfur oxides will corrode buildings, acidify soil and rivers, reduce crop production, and kill a large number of aquatic organisms. With the strengthening of people's awareness of environmental protection, the country has issued a series of laws and regulations to limit the sulfur content of vehicle fuel oil. China allowed the sulfur content of motor gasoline to be 800ppm in 2003, and by 2011, the sulfur con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C10G31/00C12R1/01
CPCC10G31/00C10G2300/202C12N1/205C12R2001/01
Inventor 冯守帅杨海麟詹晓王武辛瑜夏小乐张玲
Owner JIANGNAN UNIV
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