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Tissue culture and rapid propagation method for Acer rubrum 'Brandywine'

A tissue culture rapid propagation and red maple technology, applied in the field of plant tissue culture, can solve the problems of low seed setting rate and limited seedlings, etc., and achieve the effect of rapid growth and strong adaptability

Inactive Publication Date: 2015-06-03
罗焕荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the American red maple is mainly propagated by seed sowing, but the seed setting rate is low, which limits the supply of a large number of seedlings of the American red maple.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Disinfection of explants: Rinse the collected stems with clean water for 10 minutes and gently brush off the impurities on them with a brush. Disinfect them with 75% ethanol solution for 10 seconds in an ultra-clean workbench, and then wash them with sterile water for 3 seconds. Then, sterilize with 0.1% mercuric chloride solution for 10 minutes, rinse with sterile water for 4 times, absorb the water with sterile filter paper, and set aside.

[0018] (2) Induction culture: Cut the sterilized stem section in step (1) into a 1.5cm-long stem section and inoculate it into the induction medium for bud induction culture. After inoculation, culture in total darkness at 25°C for 5 days, and then place in light for 10 hours a day with a light intensity of 2000lx and culture at a temperature of 25°C until adventitious buds form, with an average contamination rate of only 4.5 %, the average survival rate was 89.7%, and the induction rate was 85.3%. The induction medium is: WP...

Embodiment 2

[0023] (1) Disinfection of explants: Rinse the collected stems with clean water for 15 minutes and gently brush off the impurities on them with a brush. Disinfect them in 75% ethanol solution for 20 seconds in an ultra-clean workbench, and then wash them with sterile water for 5 minutes. Then, sterilize with 0.1% mercuric chloride solution for 16 minutes, rinse with sterile water for 6 times, and then use sterile filter paper to absorb the water for later use.

[0024] (2) Induction culture: Cut the sterilized stem section in step (1) into a 1.5cm-long stem section and inoculate it into the induction medium for bud induction culture. After inoculation, culture in total darkness at 27°C for 7 days, and then place in light for 14 hours a day with a light intensity of 3000 lx and culture at a temperature of 27°C until adventitious buds form, with an average contamination rate of only 5.3 %, the average survival rate was 90.4%, and the induction rate was 86.7%. The induction medi...

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Abstract

The invention discloses a tissue culture and rapid propagation method for Acer rubrum 'Brandywine'. The Acer rubrum 'Brandywine' is an Aceraceae Acer tall deciduous tree, has the characteristics of high adaptability, drought resistance, temperature resistance, growth fastness and the like and is a fine tree species for landscaping. At present, the Acer rubrum 'Brandywine' mainly propagates seedlings with a seed sowing propagation method, but the seed fruiting rate is lower, so that supply of a large number of seedlings of the Acer rubrum 'Brandywine' is limited. According to the method, stems with buds are used as explants, tissue-cultured regenerated plants of the Acer rubrum 'Brandywine' are successfully obtained through steps of induction, multiplication, rooting, acclimatization, transplantation and the like, a systematic tissue-cultured plant regeneration system for the Acer rubrum 'Brandywine' is established, and a reference is provided for mass factory culture of the seedlings of the Acer rubrum 'Brandywine'.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for tissue culture and rapid propagation of American red maple. Background technique [0002] American red maple (Acer rubrum' Brandywine '), also known as red maple, North American red maple, Canadian red maple, northern red maple, brown sugar maple, scarlet maple, swamp maple, etc., is a tall deciduous tree belonging to the Aceraceae family. With the characteristics of temperature resistance and rapid growth, it is an excellent tree species for landscaping. The new leaves are red in spring, reflecting each other with clusters of red flowers; the branches and leaves are shaded in summer: the leaves are bright red and turn yellow in autumn, and finally bright red, which lasts for a long time. The tree shape is upright and upward, with a round crown and gray bark. Rich in color throughout the year, blooming in spring, monoecious, red f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 罗焕荣
Owner 罗焕荣
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