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Rhodococcus sp. strain and application thereof

A technology of Rhodococcus and strains, applied to bacteria, fixed on/in organic carriers, water/sludge/sewage treatment and other directions, can solve the problems of low degradation rate, low tolerance to phenolic concentration, restriction and the like, Achieving broad application prospects and strong adaptability

Active Publication Date: 2015-06-03
浙江至美环境科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Based on the above scientific and patent documents, although a variety of microorganisms screened out can degrade phenol to a certain extent, the environmental conditions for degradation are harsh, the degradation rate is low, and the concentration of phenols is low, so the promotion to practical application is greatly restricted.

Method used

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  • Rhodococcus sp. strain and application thereof
  • Rhodococcus sp. strain and application thereof
  • Rhodococcus sp. strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Isolation and identification of Rhodococcus sp. CM-HZX1

[0034] 1. Isolation of Rhodococcus sp. CM-HZX1

[0035] Rhodococcus sp. CM-HZX1 was isolated from the activated sludge in the aerobic tank of Huzhou sewage treatment plant.

[0036] (1) culture medium

[0037] Enrichment medium: yeast powder 5g, peptone 10g, NaCl 10g, add distilled water to make up to 1000mL, pH: 7.0-7.2, sterilize at 121°C for 20 minutes.

[0038] Inorganic salt medium: (NH 4 ) 2 SO 4 : 1g / L, CaCl 2 : 0.1g / L, K 2 HPO 4 : 0.5g / L, KH 2 PO 4 : 0.5g / L, MgSO 4 : 0.5g / L, NaCl: 1g / L, a certain amount of phenol, constant volume with phenol-free distilled water, pH: 7.0~7.2, sterilized at 121°C for 20 minutes.

[0039] Solid medium: add 1.5-2% agar to the above liquid medium components.

[0040] (2) Isolation and purification of bacterial strains

[0041] Take 10g of activated sludge and inoculate it into 100ml enriched medium with a phenol concentration of 100mg / L, shake it on a sha...

Embodiment 2

[0062] The best growth condition optimization of embodiment 2 Rhodococcus (Rhodococcus sp.) CM-HZX1

[0063] Medium: (NH 4 ) 2 SO 4 : 1g / L, CaCl 2 : 0.1g / L, K 2 HPO 4 : 0.5g / L, KH 2 PO 4 : 0.5g / L, MgSO 4 : 0.5g / L, NaCl: 1g / L, a certain amount of phenol, constant volume with phenol-free distilled water, pH: 7.0~7.2.

[0064] (1) Effect of the initial pH value of the medium on the degradation efficiency of phenol by Rhodococcus sp. CM-HZX1

[0065] By changing the pH value in the medium, the influence of the initial pH on the degradation efficiency of phenol by Rhodococcus sp. CM-HZX1 was investigated. Prepare 300 mL of phenol culture medium with a concentration of 500 mg / L, and the transfer volume is about 15%. Centrifuge the sludge and add it to the phenol culture medium with a pH of 3-11. For blank comparison, under the conditions of 30°C and 150r / min shaker culture, the supernatant was centrifuged for a certain period of time and the value of volatile phenol was de...

Embodiment 3

[0073] The salt resistance of the Rhodococcus strain of embodiment 3 degrading phenol

[0074] In complex industrial phenol-containing wastewater, the salt content is relatively high. By changing the salt content in the medium, the salt-resistant ability of Rhodococcus strains that degrade phenol was investigated. Prepare a phenol culture medium with a concentration of about 500mg / L, and transfer the volume to about 15%. Centrifuge the sludge and add it to the phenol culture medium. The initial pH value is 7. Cultivate in a shaker at 30°C and 150r / min, with a certain period of time. , and centrifuged to get the supernatant to detect the value of volatile phenol by direct spectrophotometry with 4-aminoantipyrine.

[0075] Depend on Figure 6 It can be seen that when the salt content of the strain of the present invention is 0.5-4%, the phenol degradation rate is about 90%, indicating that the microorganism belongs to moderate salt-tolerant microorganism.

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Abstract

The invention discloses a rhodococcus sp. strain and application thereof, belonging to the field of environmental microbiological application. The strain is named as (Rhodococcus sp.) CM-HZX1 and is now collected with the serial number of CCTCC (China Center For Type Culture Collection) NO:M 2014329 in CCTCC on July 9th, 2014. The rhodococcus sp.CM-HZX1 has relatively strong phenol degrading capacity, namely that the degradation rate is up to 93.6% after 0.5g / L of phenol is degraded for 24 hours, and the degradation rate is up to more than 90% after 1.5g / L of phenol is degraded for 48 hours. The strain is capable of tolerating the salinity of 4% and strong in adaptability; and the strain is efficient when being used for biological treatment of phenol wastewater so as to be wide in application prospect.

Description

technical field [0001] The invention relates to the application field of environmental microorganisms, in particular to a rhodococcus strain and its application. Background technique [0002] Phenol-containing wastewater mainly comes from oil refining, coking, gas, and chemical, papermaking, smelting, textile printing and dyeing production processes that use phenol or phenolic formaldehyde as raw materials. This kind of wastewater has a wide range of sources, large water volume, complex components, and high toxicity. In water pollution control, it is listed as one of the key toxic and harmful wastewater to be solved. [0003] At present, the treatment methods of phenolic wastewater mainly include physical, chemical and biological methods. The physical methods are adsorption and extraction methods, but the selectivity is poor and it is easy to cause secondary pollution. Chemical methods mainly include chemical oxidation and chemical precipitation, but the treatment costs ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/08C12N11/04C02F3/34C12R1/01C02F101/34
CPCC02F3/34C02F2101/345C12N1/20C12N11/04C12N11/08C12N11/10C12N1/205C12R2001/01
Inventor 魏霞谢柳
Owner 浙江至美环境科技有限公司
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