Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle

A composite nanoparticle and near-infrared light technology, applied in the field of biochemistry, can solve the problems of large tissue and cell damage, hinder application, and weak tissue penetration, and achieve the effect of strong penetration and little damage to cells and tissues.

Inactive Publication Date: 2015-06-03
CENT SOUTH UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most existing organic H 2 S donors require UV light to generate H 2 S, ultraviolet light is very harmful to

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle
  • Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle
  • Composite nanoparticle for controlling H2S release by employing near-infrared light as well as preparation method and application of composite nanoparticle

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0041] Example 1

[0042] Organic H 2 Chemical synthesis of S donor molecules:

[0043] The synthetic route is as follows:

[0044]

[0045] The specific steps are as follows:

[0046] (1) Weigh 3g (0.0142mol) of compound I and dissolve it in 15mL of anhydrous dichloromethane, and slowly drop 7mL under ice bath to a concentration of 2molL -1 The color of the solution changed from yellow to orange. After 2 hours of reaction, the reaction solution was poured into hydrochloric acid diluted with ice water. The crude product is obtained by filtration, and the crude product is separated by column chromatography to obtain compound II. The yield was 50.7%.

[0047] (2) Weigh 1.63 g of compound II and dissolve in 15 mL of dichloromethane, and stir in an ice bath. A measure of 1.367 mL of phosphorus tribromide was slowly added to the above solution. After reacting for 1.5 hours in an ice bath, transfer to room temperature and continue the reaction for 1.5 hours. After the completion of the r...

Example Embodiment

[0051] Example 2

[0052] Up-conversion nanoparticles UCNPs (β-LiYF4: 0.5% Er3 + ,25%Yb3 + ) Preparation:

[0053] Put the rare earth oxide (Y 2 O 3 :Yb 2 O 3 :Tm 2 O 3 = 74.5%: 25%: 0.5%) was dissolved in an appropriate amount of trifluoroacetic acid, stirred at 80°C for 5 hours, and the trifluoroacetic acid was evaporated to obtain a white powder solid. This mixed powder is dissolved in a mixed solution of oleic acid and octadecene, and then 8 times the amount of lithium fluoride is added. Heat to 110°C in a vacuum environment and react for 1 hour. Under the protection of nitrogen, increase the temperature to 320-330°C at 25°C / min, stir vigorously, and cool to room temperature after reacting for 1 hour. Add an appropriate amount of acetone and centrifuge at 6000 rpm for 10 min to obtain a white precipitate. After washing twice with acetone and absolute ethanol, it was dissolved in chloroform. The excess lithium fluoride was removed by centrifugation at 2000 rpm for 1 min, and...

Example Embodiment

[0065] Example 3

[0066] Determination of the number of SP molecules on the surface of UCNPs:

[0067] (1) Drawing of UV standard curve: configure SP molecular solutions of different concentrations: 10μgmL -1 , 12.5μgmL -1 , 16.5μgmL -1 , 22.5μgmL -1 , 28.5μgmL -1 , 38.5μgmL -1 , 50μgmL -1 , Measure the intensity of UV absorption, draw the UV standard curve, see Figure 7 a.

[0068] (2) Measure 0.25mL SP-PEG-UCNPs solution, mix with 0.75mL acetonitrile solution, measure the UV absorption intensity at 350nm, it is A 350nm = 0.093. Bringing into the UV standard curve fitting function, the concentration of SP molecules in the solution is 7.71 μgmL -1 . That is, there are 7.25×10 in 1mLSP-PEG-UCNPs solution 16 SP molecules.

[0069] (3) Take 1 mL of pure UCNPs, dry and weigh it, to 20 mg. The volume of a UCNPs is known to be 1.72×10 -16 cm 3 , The density of UCNPs is 4gcm -1 , Calculated to be 2.91×10 in 1mL solution 13 UCNPs.

[0070] (4) Finally, it is calculated that there are 250...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a composite nanoparticle for controlling H2S release by employing near-infrared light as well as a preparation method and an application of the composite nanoparticle. The composite nanoparticle is formed by loading organic H2S donor molecules on an up-conversion nanoparticle surface through a polyethylene glycol-oleylamine medium; and the preparation method is that the composite nanoparticle is obtained by chemical reaction of the up-conversion nanoparticle, the polyethylene glycol-oleylamine and the organic H2S donor molecules. The composite nanoparticle is good in stability; H2S can be released under near-infrared light illumination; the release rate can be regulated and controlled; the composite nanoparticle is good in cell compatibility, and small in cytotoxicity; the composite nanoparticle and the cell are cultured together, so that the donor cell, which can release the H2S under the near-infrared light illumination, and of which the release speed can be regulated and controlled can be obtained; and the composite nanoparticle is of great significance in the field of biological research.

Description

technical field [0001] The invention relates to a method of utilizing near-infrared light to control H 2 The novel composite nanoparticle released by S and its preparation method and application belong to the field of biochemistry. Background technique [0002] All the time, H 2 S is considered a poisonous gas. It not only has a strong irritating effect on the eyes and respiratory system of mammals, but if inhaled in large quantities, it may cause confusion, heart failure and other physiological reactions. However, recent studies have shown that H 2 S, like CO and NO, is a biological gas molecule, which has very important physiological significance. h 2 S has high reactivity with oxygen-containing substances and nitrogen-containing substances, such as hydrogen peroxide, peroxide, superoxide, nitroso, etc., and is an important antioxidant and scavenger in organisms. More importantly, H 2 S can undergo sulfhydryl reaction with cysteine ​​and connect to the iron center o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/09C07C323/16C07C319/14
Inventor 邓留陈民陈万松刘又年
Owner CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap