Novel bunyavirus reverse transcription loop-mediated isothermal amplification detection method and kit

Abstract

The invention relates to a novel bunyavirus reverse transcription loop-mediated isothermal amplification (RT-LAMP) detection method and kit. The invention aims at that the provided method and detection kit should has the characteristics of being good in specificity, high in detection sensitivity, stable, accurate, high in detection efficiency, quick and convenient to operate, and so on. Technical scheme: a novel bunyavirus reverse transcription loop-mediated isothermal amplification detection kit is provided, in the kit, RT-LAMP reaction liquid comprises the following constituents: specific amplification primer, deoxyribonucleoside triphosphate, glycine betaine, buffer solution, potassium chloride, ammonium sulfate, magnesium sulfate, Twain-20, DNA polymerase, reverse transcriptase, SYBR Green I wax microcrystalline particles and water without RNase I . The novel bunyavirus reverse transcription loop-mediated isothermal amplification detection method is carried out according to the following steps: (1) extracting RNA of a to-be-detected sample; (2) preparing a one-step-process reverse transcription loop-mediated isothermal amplification system; (3) judging result.

Description

technical field [0001] The present invention relates to a virus ring-mediated reverse transcription isothermal amplification detection method and a detection kit, especially a nucleic acid ring-mediated reverse transcription isothermal amplification detection method and a detection kit of a new type of bunyavirus, which can be applied to Rapid laboratory test for novel bunyaviruses. Background technique [0002] Fever with thrombocytopenia syndrome caused by a new type of bunyavirus infection in humans is a new infectious disease with fever accompanied by leukopenia, thrombocytopenia and multiple organ dysfunction as the main clinical manifestations. After infection with the new bunyavirus, it can rapidly cause damage to the liver and kidney function of the patient, causing the patient to die due to disseminated intravascular coagulation, shock, and respiratory failure, with a mortality rate as high as 30%. Epidemiological studies have shown that the distribution of new bun...

AI Technical Summary

Problems solved by technology

[0003] People infected by the new bunyavirus often have acute onset and high mortality, but the current treatment for the new bunyavirus infection is mainly symptomatic and supportive treatment

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