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A method for determining the amount of benzopyrene deposited in the lungs of rats after smoking by using high performance liquid chromatography

A technology of high performance liquid chromatography and benzopyrene, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of few changes in cell antioxidant enzymes and no reported detection methods, etc., and achieve good repeatability and recovery rate , shortened processing time, and convenient operation

Active Publication Date: 2017-05-03
CHINA TOBACCO ZHEJIANG IND
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Problems solved by technology

Most of the previous studies on the effect of B(a)P on respiratory diseases focused on the formation of BPDE-DNA and the molecular mechanism of lung cancer induction, while the research on B(a)P caused oxidative damage to respiratory epithelial cells. There are few studies on the changing law of
The establishment of a detection method for B(a)P pulmonary deposition after smoking will provide a good technical support for in-depth research on smoking and lung hazards. There is no report on the detection method of B(a)P pulmonary deposition after smoking. Research

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  • A method for determining the amount of benzopyrene deposited in the lungs of rats after smoking by using high performance liquid chromatography
  • A method for determining the amount of benzopyrene deposited in the lungs of rats after smoking by using high performance liquid chromatography

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Embodiment Construction

[0019] To establish a method for the detection of benzopyrene in the lungs of rats after smoking by high performance liquid chromatography. Rats smoked in their mouths and noses. The cigarette samples were commercially available cigarettes with a tar content of 11 mg. They were smoked in the international standard mode, and 10 and 20 cigarettes were smoked respectively. After smoking, the rats were killed, and the homogenate of the lung tissue was taken, extracted with dimethyl sulfoxide and cyclohexane, concentrated, and used a C18 reverse column with acetonitrile and water as the mobile phase, and a fluorescence detector was used to detect benzopyrene content.

[0020] 1. Main instruments and reagents

[0021] Main instruments: electronic balance: Shanghai Tianping Instrument Factory, FA1104; high performance liquid chromatography: Shimadzu LC-20; chromatographic column: BP-C18 column (250mm*4.6mm); refrigerated centrifuge: Thermo, ST16R; rotary evaporator Instrument: Nanj...

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Abstract

The invention relates to a method for determining deposition amount of benzopyrene in rat lung after smoking by virtue of high performance liquid chromatography. The method comprises five steps as follows: (1) inducing a rat to smoke; (2) preparing a to-be-detected sample solution; (3) preparing a standard working solution; (4) determining chromatographic conditions; and (5) drawing a standard curve and calculating a result. The method for determining deposition amount of benzopyrene in rat lung after smoking by virtue of high performance liquid chromatography disclosed by the invention has the following advantages: 1. through cigarette smoke generated from smoking by virtue of a smoking machine, deposition of benzopyrene in rat lung under different smoking modes of different cigarettes can be detected, and the operation is flexible; 2. compared with a conventional method adopting Soxhlet extraction, the experiment has the advantages that the sample processing time can be shortened greatly and the operation is convenient in a mode of cyclohexane extraction-dimethyl sulfoxide reextraction-cyclohexane extraction; and 3. the method disclosed by the invention has the advantages of high sensitivity and good repeatability and recovery rate.

Description

technical field [0001] The invention relates to the technical field of biological testing of smoke compounds, in particular to a method for measuring the amount of benzopyrene deposited in the lungs of rats after smoking by using high-performance liquid chromatography. Background technique [0002] Benzo(a)pyrene (B(a)P) not only widely exists in the natural environment, but also is the most important harmful substance in cigarette smoke. my country is the country with the largest number of smokers in the world, and there is a trend of rejuvenation. The smoking rate of men has reached 63%. Although the smoking rate of women in my country is only 3.8%, more than half of them have a history of passive smoking. The impact of benzopyrene on the human respiratory system has important clinical significance. Studies have shown that a variety of lung diseases are closely related to the toxicological effects of benzopyrene. The formation of adducts causes DNA damage: On the other ha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 周国俊储国海肖卫强胡安福李霞尹洁蒋健刘金莉卢昕博袁凯龙胡雅军王韵许成云吴希美
Owner CHINA TOBACCO ZHEJIANG IND
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