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Melamine, quinolones two-in-one gold standard microporous test strip

A melamine and quinolone technology, applied in the directions of microorganisms, biochemical equipment and methods, instruments, etc., can solve the problems such as the lack of simultaneous detection of melamine and quinolone residues, large sensitivity impact, and unsuitability for screening. The effect of a wide range, broad market prospects and strong adaptability

Active Publication Date: 2017-01-11
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Most of the existing detection methods for quinolones and melamine are chromatographic methods. The sensitivity of these methods is greatly affected by steps such as sample purification and concentration. Moreover, these methods require complex instruments and the process is cumbersome, and they are not suitable for the screening of a large number of samples on site. check
At present, there is no product that simultaneously detects the residues of melamine and quinolones

Method used

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  • Melamine, quinolones two-in-one gold standard microporous test strip
  • Melamine, quinolones two-in-one gold standard microporous test strip
  • Melamine, quinolones two-in-one gold standard microporous test strip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1, the preparation of the colloidal gold marker of conjugate, specific antibody and specific antibody

[0056] 1. Preparation of conjugates

[0057] 1. Preparation of melamine-carrier protein conjugates

[0058] (1) Take 50mg of melamine and dissolve it in 2ml DMSO.

[0059] (2) Take 50mg of HSA and dissolve it in 3ml of PBS buffer (pH7.2, 0.01M).

[0060] (3) Mix the melamine solution obtained in step (1) and the HSA solution obtained in step (2) evenly, then add 1ml of 5% (volume ratio) glutaraldehyde aqueous solution drop by drop and stir at room temperature for 3h, then add 1ml of 20mg / ml hydroboration Sodium aqueous solution was kept at 4°C for 1 hour, then placed in a dialysis bag and dialyzed with pure water (dialysis parameters: 4°C, stirring at 100 rpm, for 2 days, changing the solution twice a day), and then with PBS buffer (pH7.2, 0.01M) for dialysis (dialysis process parameters: 4°C, 100rpm stirring, 2 days, twice a day), then centrifuge at 450...

Embodiment 2

[0092] Embodiment 2, the assembly of kit

[0093] figure 1 It is a schematic diagram of the structure of the test strip. figure 2 It is a schematic diagram of the use state of the kit. image 3 Schematic diagram for the interpretation of the results of the kit.

[0094] The kit for detecting melamine and quinolones provided by the present invention includes the sample plate prepared in Step 3 of Example 1 and 8 identical test strips. The kit also includes an aluminum foil bag, and the sample plate and the test strip are packaged in the aluminum foil bag. Each test strip includes a bottom plate 1 , a reaction film 2 , a sample absorbent pad 3 and a water absorbent pad 4 . The sample absorbent pad, the reaction membrane and the water absorbent pad are attached to the bottom plate in sequence, the end of the sample absorbent pad is connected to the beginning of the reaction membrane, and the end of the reaction membrane is connected to the water absorbent pad. connected at ...

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Abstract

The invention discloses a melamine and quinolone two-in-one gold-labeled microporous test paper strip. A kit provided by the invention comprises a sample board and at least one test paper strip, wherein the sample board is at least provided with one sample groove; the bottom of each sample groove is attached with a colloidal gold label of a melamine specific antibody and a colloidal gold label of a norfloxacin specific antibody. Each test paper strip comprises a bottom plate (1), a reaction film (2), a sample absorption pad (3) and a water absorption pad (4), wherein the corresponding sample absorption pad, the corresponding reaction film and the corresponding water absorption pad are sequentially attached to the corresponding bottom plate; each reaction film is sequentially provided with a T2 line, a T1 line and a C line from the initial end to the tail end, and the corresponding T2 line and the corresponding T1 line are respectively coated with the following two kinds of conjugates, namely a norfloxacin-carrier protein conjugate and a melamine-carrier protein conjugate; the corresponding C line is coated with an antiantibody. The kit provided by the invention has a broad market prospect and relatively great economic and social benefits.

Description

technical field [0001] The invention belongs to the field of food safety testing, and in particular relates to a two-in-one gold standard microporous test strip for simultaneously detecting melamine and quinolone residues in animal-derived foods (such as milk, etc.). Background technique [0002] Melamine (MEL), referred to as triamine, IUPAC named "1,3,5-triazine-2,4,6-triamine", scientific name triamine triazine, alias melamine, cyanuramide, trimer Amide is an important nitrogen-heterocyclic organic chemical raw material. It is a white monoclinic crystal, almost odorless, slightly soluble in water, soluble in methanol, formaldehyde, acetic acid, hot ethylene glycol, glycerin, pyridine, etc., insoluble in Acetone and ethers are harmful to the body and cannot be used in food processing or food additives. Melamine is a basic organic chemical intermediate product with a wide range of uses, and its main use is as a raw material for the production of melamine-formaldehyde resin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/558C12N5/20C07K16/44
CPCG01N33/558
Inventor 沈建忠王战辉江海洋丁双阳张素霞史为民吴聪明曹兴元夏曦
Owner CHINA AGRI UNIV