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Method for preparing persimmon fruit pigments

A pigment and persimmon technology, applied in the field of preparation of plant natural pigments, can solve the problem of low pigment content, achieve uniform particle size distribution, increase product yield, and shorten extraction time

Inactive Publication Date: 2015-06-10
桂林得坤生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Chinese patent CN103013164A discloses a method for extracting natural pigments from persimmon skins, using the peels removed from persimmon production as raw materials, using countercurrent extraction combined with macroporous adsorption resin separation and extraction to separate the persimmon skin pigments, and countercurrent extraction using heated solvents to reverse The raw material of persimmon skin is extracted, and the natural pigment components can be selectively adsorbed by macroporous adsorption resin, but the pigment content obtained by this method is low

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  • Method for preparing persimmon fruit pigments

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Effect test

Embodiment 1

[0026] A preparation method for persimmon fruit pigment, comprising the steps of:

[0027] 1) Raw material processing: wash and crush 10kg of persimmons;

[0028] 2) High-pressure micro-jet micro-grinding treatment: add 50kg of pure water to the crushed persimmons, process it with a high-speed shear emulsifier for 10 minutes at a speed of 12000r / min, and then use a high-pressure micro-jet ultra-fine pulverizer to process it at 25°C , the treatment pressure is 120MPa, and the number of treatments is 3 times to obtain a paste slurry;

[0029] 3) Fermentation: The paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 7.0, inserted into the cultured mature Lactobacillus bulgaricus liquid strain at 10% by volume, and put into a fermenter for fermentation , the fermentation temperature is 35-37°C, and the fermentation time is 4h;

[0030] 4) Extraction: The fermented liquid obtained in step 3) is subjected to ultra...

Embodiment 2

[0037] A preparation method for persimmon fruit pigment, comprising the steps of:

[0038] 1) Raw material processing: wash and crush 10kg of persimmons;

[0039] 2) High-pressure micro-jet ultra-fine pulverization treatment: add 60kg of pure water to the crushed persimmons, process them with a high-speed shear emulsifier for 20 minutes at a speed of 10,000r / min, and then use high-pressure micro-jet ultra-fine pulverization equipment for processing at 25°C , the treatment pressure is 100MPa, and the number of treatments is 2 times to obtain a paste slurry;

[0040] 3) Fermentation: the paste-like slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, then adjusted to pH 6.5, and 12% by volume is inserted into a mature Lactobacillus acidophilus liquid strain, and placed in a fermenter Fermentation, fermentation temperature is 35-37℃, fermentation time is 3h;

[0041] 4) Extraction: the fermentation broth obtained in step 3) is subjected t...

Embodiment 3

[0048] A preparation method of persimmon fruit pigment, comprising the following steps:

[0049] 1) Raw material treatment: 10kg persimmons are cleaned and broken;

[0050] 2) High-pressure micro-jet ultra-fine pulverization treatment: add 50kg of pure water to the crushed persimmons, use a high-speed shearing emulsifier for 15 minutes, the rotating speed is 8000 r / min, and then use a high-pressure micro-jet ultra-fine pulverization equipment at 25 ° C for processing , the treatment pressure is 80MPa, and the treatment times are 2 times to obtain a paste-like slurry;

[0051] 3) Fermentation: the paste slurry obtained in step 2) is subjected to ultra-high temperature instantaneous sterilization, and then adjusted to pH 7.0, 15% by volume percentage is connected to the cultured mature Streptococcus crema liquid strain, put into a fermenter for fermentation , the fermentation temperature is 35-37℃, and the fermentation time is 4h;

[0052] 4) Extraction: the fermentation broth...

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Abstract

The invention discloses a method for preparing persimmon fruit pigments. The method comprises the steps of raw material processing, high pressure microfluidics superfine grinding processing, fermentation processing, extraction, macroporous resin column chromatography, polyamide column chromatography, absorption and purification of pigments solution by sephadex gel filtration chromatography and separation and purification of persimmon fruit pigments by preparative liquid chromatography, by means of the method, the water solubility impurities in the pigments solution can be effectively removed, the primary separation of pigment monomers is achieved, the separation of monomers of the persimmon fruit pigments is effectively achieved, and the purity of a product is guaranteed.

Description

technical field [0001] The invention relates to a preparation method of plant natural pigment, in particular to a preparation method of persimmon fruit pigment. Background technique [0002] Natural colors are food colors obtained from natural sources. Pigments mainly extracted from animal and plant tissues and microorganisms (cultivation), among which vegetable colorants account for the majority. Natural pigments not only have the function of coloring food, but also a considerable part of natural pigments have physiological activity. [0003] Persimmon (Diospyros.kaki.L), also known as monkey date, red fruit, persimmon tree is a perennial deciduous fruit tree. Persimmon is a plant of the genus Diospyros.kaki.L in the family Ebenaceae. There are more than 500 species of this genus in the world, most of which are tropical or subtropical plants, and a few are produced in warm temperate regions. Persimmon is a temperate deciduous fruit tree of Persimmonaceae berry class. It ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00
Inventor 刘小峰何明茜罗知颂李京生刘二伟
Owner 桂林得坤生物科技股份有限公司
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