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Schizochytrium sta‑m‑3 and its use and method of producing Schizochytrium

A Schizochytrium and microorganism technology, applied to Schizochytrium STA-M-3 and its application field, can solve the problem of high price of DHA products, achieve shortened production cycle, single unsaturated fatty acid composition, and good genetic stability Effect

Active Publication Date: 2018-02-09
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Another object of the present invention is to provide a method utilizing Schizochytrium to produce DHA; Schizochytrium STA-M-3 provided by the invention produces DHA with starch as a carbon source, which solves the problem that the price of DHA products is relatively high question

Method used

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  • Schizochytrium sta‑m‑3 and its use and method of producing Schizochytrium
  • Schizochytrium sta‑m‑3 and its use and method of producing Schizochytrium
  • Schizochytrium sta‑m‑3 and its use and method of producing Schizochytrium

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Embodiment 1

[0026] A kind of Schizochytrium STA-M-3, which is classified as Schizochytrium, and the Schizochytrium is preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee, and the preservation number is: CGMCC No.9381 , The preservation time is: June 25, 2014, and the address of the preservation unit is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences.

Embodiment 2

[0028] The Schizochytrium STA-M-3 is based on the DHA high-yield strain Schizochytrium B4D1 and the high-efficiency use of starch niger CGMCC3.316 as the starting strain, using the method of inactivating the parents and protoplast fusion to make the two parents The protoplasts are fused and regenerated to obtain strains with both parental traits.

[0029] 1. Experimental reagents and culture medium:

[0030] Buffer solution PBS: disodium hydrogen phosphate-sodium dihydrogen phosphate, the concentration is 0.2mol / L, pH5.8, sterilized at 121°C for 20min.

[0031] Hypertonic buffer HPBS: Add 0.7mol / L KCl to PBS buffer.

[0032] Pretreatment agent: add 0.1% EDTA to PBS buffer, and sterilize at 121°C for 20min. Add 0.3% β-mercaptoethanol just before use. Enzyme solution: Lysing enzyme (Lysing enzyme) was purchased from Sigma Company (it is better to write down the model and specification). Weigh the lyase, suspend it in HPBS, vibrate fully and let it stand still to dissolve it ...

Embodiment 3

[0055] The invention discloses a method for producing DHA by using Schizochytrium STA-M-3. The Schizochytrium STA-M-3 is cultivated in a starch medium to produce DHA.

[0056] The starch medium uses seawater or artificial seawater as an inorganic salt source, with a salinity of about 15‰, starch as a carbon source, with a concentration of 5g / L, and corn steep liquor, yeast extract or peptone as a nitrogen source, with a concentration of 2g / L, cultivated at 28°C for 7 days, and the pH was in the range of 4-8 during the cultivation process.

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Abstract

The invention discloses a Schizochytrium (Schizochytrium) STA-M-3. The Schizochytrium is preserved in the General Microorganism Center of the China Microbiological Culture Collection Management Committee, the preservation number is: CGMCC No.9381, and the preservation time is : June 25, 2014. The invention also discloses a method for producing Schizochytrium, comprising the following steps: step 1, using Schizochytrium B4D1 and Aspergillus niger CGMCC3.316 as two parents, and preparing the protoplasts of the two parents; The protoplasts of the parents are fused; step 3, inoculating the fused protoplasts on a starch medium and culturing them to obtain Schizochytrium STA-M-3. The invention also discloses a method for producing DHA by utilizing the schizochytrid fungus, wherein the schizochytrid fungus STA‑M‑3 is cultivated in a starch medium to produce DHA. The invention solves the problem of relatively high price of DHA products.

Description

technical field [0001] The invention belongs to the field of industrial biotechnology, relates to a protoplast fusion technology, in particular to a method for constructing a new type of Schizochytrium which directly utilizes starch to produce DHA through protoplast fusion, in particular to Schizochytrium STA-M-3 and its application. Background technique [0002] DHA (Docosahexaenoic acid, docosahexaenoic acid) is a polyunsaturated fatty acid with important physiological significance and economic value. DHA is not only an essential substance for the normal development of the brain and vision of infants, but also plays a positive role in delaying the decline of brain and vision functions in the elderly, reducing blood lipids, inhibiting thrombosis, regulating immune function, inhibiting tumors, and preventing and treating inflammatory diseases. Compared with traditional fish oil sources, DHA prepared by microbial fermentation has many advantages such as single unsaturated fa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/15C12N15/04C12P7/64C12R1/645
CPCC12N1/14C12N15/04C12P7/6427C12N1/145C12R2001/645
Inventor 李德茂王迪陈树林张可王海军陈吴西
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI