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A kind of extraction method and application of myrrh leaf cell extract

An extraction method and extract technology are applied in the directions of pharmaceutical formulations, cosmetic preparations, preparations for skin care, etc., which can solve the problems of low yield of flavonoids, large consumption of organic solvents, damage to cells, etc. Easy to use effect

Active Publication Date: 2018-06-05
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the alcohol extraction method refers to the method of utilizing the solubility of ethanol and using ethanol as a solvent to separate and purify substances; however, the yield of flavonoids extracted by this method is low, the consumption of organic solvent is large, and the separation difficulty is high, and the operation steps are many; Ultrasonic extraction method is to use ultrasonic assisted solvent for extraction. The sound wave produces high-speed, strong cavitation effect and stirring effect, destroying the cells of the herbal medicinal materials, allowing the solvent to penetrate into the medicinal material cells, shortening the extraction time and improving the extraction rate, but it is not easy to extract in Widely produced in industry

Method used

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  • A kind of extraction method and application of myrrh leaf cell extract

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Experimental program
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Effect test

Embodiment 1

[0044] ① Pretreatment of myrrh: wash the leaves of myrrh, dry them naturally in the shade, dry them at a constant temperature of 50°C, and crush them through a 60-mesh sieve;

[0045] ② Mixing: Weigh 10 g of myrrh leaves, put them into a conical flask, add 0.4 g of Tween-80 and 0.1 g of cellulase and water, the mixed solution is 1 L in total, mix and stir evenly, so that the myrrh leaves and Tween-80 Mix well with cellulase;

[0046] ③Enzymolysis: Put the triangular flask containing myrrh leaf powder, Tween-80, and cellulase in a water bath, the temperature of the water bath is 70°C, and the vacuum degree is -0.8MPa for enzymolysis reaction for 110 minutes;

[0047] ④ Filtration: After the enzymolysis reaction is completed, filter under reduced pressure, transfer the solid-liquid mixture to filter paper, and turn on the suction pump to start suction filtration;

[0048] ⑤ Concentration of the extract: After filtering, use the method of rotating membrane concentration, and thr...

Embodiment 2

[0062] The method for extracting myrrh leaf extract in the present embodiment 2 is basically the same as that of embodiment 1, the difference is: step 2. take by weighing 150g of myrrh leaf extract, put it into a conical flask, add 10g Tween-80 and 50g cellulase and Water, a total of 1L of the mixed solution, mixed evenly and stirred, so that the leaves of myrrh were fully mixed with Tween-80 and cellulase; during the enzymatic hydrolysis in step 3, the temperature of the water bath was 40°C and the vacuum degree was -0.4MPa for 50min. In step ⑥, ethanol with a mass fraction of 75% is used for extraction.

[0063] The following is a test to verify that the flavonoids extracted from myrrh leaves have anti-aging and free radical scavenging effects: Animal experiments with male Kunming mice: 75 male Kunming mice were purchased at the age of 4 weeks , the laboratory was free to drink water and eat food for 5 days, and they were randomly divided into 5 groups, 25 in each group. Th...

Embodiment 3

[0076] Take each component by weight as follows:

[0077] Propylene glycol: 10 parts;

[0078] Myrrh leaf cell extract: 5 parts;

[0079] Hyaluronic acid: 3 parts;

[0080] POE oleyl ether: 1.5 parts;

[0081] Paraben: 0.5 parts;

[0082] Vitamin B: 0.1 parts;

[0083] Vitamin E: 0.1 parts;

[0084] Carboxymethylcellulose: 5 parts

[0085] Water: 74.8 parts

[0086] Wherein, Myrrha leaf cell extract was prepared in Example 1.

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Abstract

The invention discloses an extraction method and application of a myrrh leaf cell extract. The extraction method comprises the following steps: (1) drying and crushing myrrh leaves; (2) performing enzymolysis, to be specific, uniformly mixing myrrh leaves, cellulase, tween-80 and water, and reacting for 50-110 minutes at 40-70 DEG C in the vacuum degree of (-0.4)-(-0.8)MPa; (3) filtering and concentrating the obtained mixture; (4) extracting, to be specific, performing extraction on the concentrated liquid by using a microwave method under the conditions that the microwave output power is 200-500W and the frequency is 3000MHz, filtering the extract, drying the filter residues, and grinding the filter residues into powder, so as to obtain the myrrh leaf cell extract. An anti-aging cosmetic consisting of the myrrh leaf cell extract extracted by using the extraction method, hyaluronic acid and the like has the effects of preventing aging, removing free radicals, and the like.

Description

technical field [0001] The invention belongs to the field of biological cosmetology, and in particular relates to an extraction method of myrrh leaf cell extract and its application in skin care products. Background technique [0002] Myrrh has another name called powdered myrrh, bright myrrh, myrrh, powdered myrrh, processed myrrh, roasted myrrh, vinegar fried myrrh, raw myrrh, raw myrrh and other other names. It is the dry resin of the olive tree or hardidin tree, mainly produced in Africa, Somalia, Ethiopia and India. Gather the white oil gum resin exuded from the cracks in the bark, and turn into reddish-brown hard round lumps in the air. After smashing, fry until burnt black and apply. Cure mainly chest and abdomen stasis and pain, dysmenorrhea, amenorrhea, lump in the abdomen, bruises, carbuncle sores, intestinal abscess, red eyes and swelling. It has the effects of promoting blood circulation, relieving pain, reducing swelling and promoting muscle growth. In the E...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/99A61K8/9789A61Q19/10A61Q19/08A61Q19/02A61Q19/00
Inventor 陈海佳王一飞葛啸虎黎敏聪池诗源
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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