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Preparation method of polyprenol microemulsion with antibacterial activity

A technology of polyprenol and bacteriostatic activity is applied in the field of preparation of polyprenol microemulsion, which can solve the problems affecting research and development of biological activity, high molecular weight and low bioavailability of polyprenol, and achieves good centrifugal effect. Stability, maintaining biological activity, strong antibacterial effect

Active Publication Date: 2020-08-11
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Polyprenol has a large molecular weight and strong hydrophobicity, which leads to low bioavailability of polyprenol in humans and animals, which affects the research on its biological activity and the development of related preparations

Method used

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  • Preparation method of polyprenol microemulsion with antibacterial activity
  • Preparation method of polyprenol microemulsion with antibacterial activity
  • Preparation method of polyprenol microemulsion with antibacterial activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Single factor investigation of emulsifier HLB value

[0034] The definition formula of emulsifier HLB value is: HLB 混合 =HLB a *W a / (W a +W b )+HLB b *W b / (W a +W b ), where W a Represents the mass score of Span-80, HLB a Represents the HLB value of Span-80, W b Represents the quality score of Tween-80, HLB b Represents the HLB value of Tween-80.

[0035] Emulsification conditions: polyprenol 10 g, emulsifier 20 g, stirring speed 10000 r / min, emulsification temperature 60 ℃, stirring time 10 min, investigate the effect of the HLB value (8-12) of the compound emulsifier on the particle size of the emulsion The effect of size. After the emulsification was completed, 15 ml of the emulsions were taken out, cooled to room temperature, placed in a centrifuge at 8000 r / min for 20 min, and the stratification was observed.

[0036] HLB value State after centrifugation 8 obvious stratification 8.5 slightly layered 9 slightly ...

Embodiment 2

[0040] Embodiment 2 Single factor investigation of emulsifier and polyprenol ratio

[0041]Emulsification conditions: polyprenol 10 g, stirring speed 10000 r / min, emulsification temperature 60 ℃, stirring time 10min, investigate the ratio of compound emulsifier to polyprenol [m(emulsifier):m(polypentenol Enol) = (1 ~ 2): 1] on the particle size of the emulsion. The obtained emulsion was subjected to particle size analysis, and the results are shown in Table 3.

[0042] Ratio of emulsifier to polyprenol Average particle size (nm) 1:1 4202 1.25:1 2121 1.5:1 921 1.75:1 283 2:1 625

[0043] The results showed that the average particle size of the emulsion was the smallest when the ratio of emulsifier to polyprenol was 1.75:1, so it was determined that the ratio of emulsifier to polyprenol was 1.75:1 as the optimum value.

Embodiment 3

[0044] Example 3 Single factor investigation of stirring speed

[0045] Emulsification conditions: polyprenol 10 g, emulsifier 20 g, emulsification temperature 60 ℃, stirring time 10 min, to investigate the effect of stirring speed on emulsion particle size. The obtained emulsion was subjected to particle size analysis, and the results are shown in Table 4.

[0046] Stirring speedr / min Average particle size (nm) 5000 1360 10000 354 15000 124 20000 119

[0047] The results show that the average particle size of the emulsion is relatively close when the stirring speed is 15000 r / min and 20000 r / min, considering the economical efficiency of the actual operation, it is determined that the stirring speed is 15000 r / min is the best value.

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Abstract

The invention provides a preparation method for a polyprenol microemulsion with bacteriostatic activity. The preparation method comprises the following steps: with polyprenol as a raw material, mixing an emulsifier with a co-emulsifier and then adding polyprenol to form an oil phase; carrying out stirring and adding deionized water, wherein the obtained system temporarily becomes turbid and then becomes clear again; after stabilization of the system, continuing adding a great amount of deionized water with stirring under a heating condition so as to realize phase inversion, wherein the system turns turbid from clear; and finally allowing temperature to rise and carrying out high speed stirring for a certain period of time, wherein the polyprenol microemulsion is obtained when the system becomes clear. The invention has the following advantages: the polyprenol microemulsion with good water dispersibility is prepared from fat-soluble polyprenol grease; and the preparation method is simple, effectively improves water dispersibility, physical properties and bacteriostatic biological activity of polyprenol and broadens the application scope of fat-soluble polyprenol in the industries of food, health products and cosmetics and industries related to biological medicine.

Description

technical field [0001] The invention relates to a preparation method of polyprenol microemulsion with antibacterial activity, belonging to the field of biomedicine. Background technique [0002] Polyprenols (GBP) is based on C 5 Lipid compounds with isopentenyl as the structural unit can be divided structurally: betulin type ω-(trans) 2 -(cis) n -OH, fika alcohol type ω-(trans) 3 -(cis) n -OH and solanesol type omega-(trans) n -OH, see figure 1 . Polyprenol is an important active ingredient in the synthesis of glycoproteins in the cell membrane of organisms. It participates in various metabolisms in organisms and has a wide range of biological activities. [0003] The Latvian Academy of Medical Sciences and "BIOLAT" company have followed the "Preclinical Toxicological Test Requirements for New Drug Substances" of the Drug Committee of the Ministry of Health of the Soviet Union. The results show that polyprenol is safe and non-toxic to the human body, and has no mutage...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/107A61K31/765A61K8/06A61K8/84A61K8/9789A61K8/9771A61K8/9767A23L33/00A61K36/16A61K36/15A61K36/605A61P31/04A61Q19/00
CPCY02A50/30
Inventor 王成章陶冉
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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