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Magnetic graphene oxide nano-silver composite material and preparation and application thereof

A composite material and oxide stone technology, which is applied in the field of magnetic graphene oxide nano-silver composite material and its preparation, can solve the problems of small amount of silver immobilized, achieve the effect of fast adsorption and elution of glycopeptides, and simplify experimental operation

Active Publication Date: 2015-06-17
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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Problems solved by technology

However, the current polymer-based nano-silver composites have a small amount of silver immobilization and unavoidable non-specific adsorption, so it is necessary to develop new nano-silver composites.

Method used

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  • Magnetic graphene oxide nano-silver composite material and preparation and application thereof
  • Magnetic graphene oxide nano-silver composite material and preparation and application thereof
  • Magnetic graphene oxide nano-silver composite material and preparation and application thereof

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specific Embodiment 2

[0026] 1. Preparation of magnetic graphene oxide nano-silver composite: press figure 1 As shown, the magnetic graphene oxide was prepared by the condensation reaction between graphene oxide and aminoferric oxide. Weigh 20 mg of graphene oxide, add 40 mL of 0.1M morphine ethanesulfonic acid buffer solution (pH5.6), and ultrasonicate for 3 hours to completely peel off the graphene oxide to form a uniform dispersion, then add 192 mg of 1-ethyl-3-(3-di Methylaminopropyl)-carbodiimide and 115mg N-hydroxysuccinimide were used to activate the carboxyl group of graphene oxide by ultrasonication for 2 hours, then 100mg of aminoferric iron tetroxide was added, and the magnetic graphene oxide composite material was prepared by ultrasonic reaction for 1 hour. Magnetic adsorption, washed with deionized water and ethanol three times alternately, and vacuum dried at 60°C.

[0027] Take 3mg of magnetic graphene oxide and disperse it in 1mL of deionized water, add 30mg of polyethyleneimine, t...

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Abstract

The invention relates to preparation of a magnetic graphene oxide nano-silver composite material with glycopeptide specifically enriched. According to the covalence reaction between graphene oxide and amino ferroferric oxide, magnetic graphene oxide is prepared. The magnetic graphene oxide is used as a carrier, polyethyleneimine is used as a reducing agent and a stabilizer, and nano-silver particles are generated in situ and fixed on the surface of the magnetic graphene oxide. The magnetic graphene oxide nano-silver composite material is used as a hydrophilic stationary phase to be used for specific enrichment of glycopeptide. By means of the preparation method, the magnetic graphene oxide nano-silver composite material is successfully used for hydrophilic enrichment of glycopeptide. The magnetic graphene oxide nano-silver composite material has good application prospects and practical value in the proteomics.

Description

technical field [0001] The invention relates to a magnetic graphene oxide nano-silver composite material and a preparation method thereof, which are used for the specific enrichment of glycopeptides in hydrophilic patterns. Background technique [0002] Protein glycosylation is an important post-translational modification that plays an important role in many biological processes. At present, glycopeptide identification and analysis based on mass spectrometry technology is an important means to study glycoproteomics. However, the low abundance of glycopeptides and the strong ion suppression of non-glycopeptides seriously interfere with the identification of glycopeptides. Therefore, it is necessary to specifically enrich low-abundance glycopeptides before mass spectrometry analysis. Hydrophilic interaction chromatography has been successfully used in the enrichment of glycopeptides. Compared with other enrichment methods, hydrophilic interaction chromatography has the advant...

Claims

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Application Information

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IPC IPC(8): B22F1/00B22F9/24B01J20/20B01J20/30G01N30/08
Inventor 张丽华江波吴琪杨开广张玉奎
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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