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Method for making ginseng extraction component by multi-enzyme enzymolysis

A ginseng extraction and multi-enzyme technology, which is applied in the field of enzyme engineering, can solve the problems of low biological activity, residues that cannot be recycled, and low extraction rate, and achieve high extraction rate, shorten extraction time, and comprehensive effects of saponin components

Inactive Publication Date: 2015-06-24
珲春绿源参业生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The existing methods for extracting effective components of ginseng generally have the disadvantages of low extraction rate, low biological activity, and residues that cannot be recycled.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] S1, after getting clean ginseng and cutting into sheets with a thickness of 5 mm, carry out superfine grinding, and pass through a 100-mesh medicine sieve;

[0031] S2, after adding distilled water in a ratio of 1:8, adjust the pH value to 3.6 with citric acid and lactic acid;

[0032] S3. Place the ginseng slurry obtained in step S1 at a temperature of 30° C., and treat it at a low temperature for 10 minutes;

[0033] S4. Place the ginseng slurry obtained in step S3 in a test tube, put it into an ultrasonic water bath after stoppering, and conduct ultrasonic waves at a constant temperature for 30 minutes;

[0034] S5. Pour the ginseng slurry obtained in step S4 into an enzymatic hydrolysis tank, add a lignin degrading enzyme with an activity of 55U / mL, and enzymolyze it at 50°C for 0.7h;

[0035] S6. Add the compound enzymolysis solution at a ratio of 300.0 g / ton, and conduct enzymolysis at 35°C for 72 hours, stir and oxygenate once every 4 hours, and control the pH v...

Embodiment 2

[0038] S1, after getting clean ginseng and cutting into sheets with a thickness of 5 mm, carry out superfine grinding, and pass through a 400-mesh medicine sieve;

[0039] S2. After adding distilled water in a ratio of 1:12, adjust the pH value to 4.8 with citric acid and lactic acid;

[0040] S3. Place the ginseng slurry obtained in step S1 at a temperature of 45° C., and treat it at a low temperature for 15 minutes;

[0041] S4. Place the ginseng slurry obtained in step S3 in a test tube, put it into an ultrasonic water bath after stoppering, and conduct ultrasonic waves at a constant temperature for 30 minutes;

[0042] S5. Pour the ginseng slurry obtained in step S4 into an enzymatic hydrolysis tank, add a lignin degrading enzyme with an activity of 55-85 U / mL, and perform enzymatic hydrolysis at 60°C for 1.8 hours;

[0043] S6, 350.0 g / ton ratio is added to the compound enzymolysis solution, 35°C, enzymolysis for 72 hours, stirring and oxygenation once every 4 hours, and...

Embodiment 3

[0046] S1, after getting clean ginseng and cutting into sheets with a thickness of 5 mm, carry out superfine grinding, and pass through a 250-mesh medicine sieve;

[0047] S2. After adding distilled water in a ratio of 1:10, adjust the pH value to 4.2 with citric acid and lactic acid;

[0048] S3. Place the ginseng slurry obtained in step S1 at a temperature of 40° C., and treat it at a low temperature for 12.5 minutes;

[0049] S4. Place the ginseng slurry obtained in step S3 in a test tube, put it into an ultrasonic water bath after stoppering, and conduct ultrasonic waves at a constant temperature for 30 minutes;

[0050] S5. Pour the ginseng slurry obtained in step S4 into an enzymatic hydrolysis tank, add a lignin degrading enzyme with an activity of 55-85 U / mL, and enzymatically hydrolyze at 55°C for 1.25 hours;

[0051] S6. Add the compound enzymatic hydrolysis solution at a ratio of 375 g / ton, 35°C, enzymatic hydrolysis for 72 hours, stirring and oxygenating once ever...

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Abstract

The invention discloses a method for making a ginseng extraction component by multi-enzyme enzymolysis. The method comprises the following steps: taking a clean ginseng, cutting into slices with the thickness of 5mm, performing ultrafine crushing, adding distilled water, then regulating the pH value to 3.6-4.8 with citric acid and lactic acid, putting at the temperature of 30-45 DEG C, performing low-temperature treatment for 10-15min, putting into a test tube, plugging, then putting into an ultrasonic water bath and performing constant-temperature ultrasonic treatment for 30min; pouring into an enzymolysis tank, adding a lignin degrading enzyme with the activity of 55-85U / mL, and performing enzymolysis at 50-60 DEG C for 0.7-1.8h; adding a composite enzymolysis solution, performing enzymolysis at 35 DEG C for 72h, stirring and filling oxygen once every four hours, and controlling the pH value at 3.5-6.2; and filtering after enzymolysis, adding ethanol into residues for leaching, filtering, merging filtrate, and performing spray drying to obtain powder. The process disclosed by the invention is simple, convenient and easy to operate and high in extraction rate.

Description

technical field [0001] The invention relates to the technical field of enzyme engineering, in particular to a method for producing ginseng extract components through multi-enzyme enzymolysis. Background technique [0002] Ginseng (Panax ginseng C.A.Mey) is the dried root of the Araliaceae plant ginseng, commonly known as ginseng, ghost cover, goblin, baby ginseng, etc. Ginseng is mild in nature, sweet in taste, slightly bitter and lukewarm. It has the effects of invigorating vital energy, recovering pulse and strengthening the spleen, nourishing the spleen and lung, promoting body fluid and quenching thirst, calming the nerves and improving intelligence. In recent years, scholars at home and abroad have conducted pharmacological studies, clinical observations and epidemiological examinations on the active ingredients of ginseng. Radiation, anti-fatigue and other biological activities. [0003] The chemical components of ginseng mainly include triterpenoid ginsenosides, org...

Claims

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Application Information

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IPC IPC(8): A61K36/258
Inventor 曲新砉
Owner 珲春绿源参业生物科技有限公司
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