Method for recycling nitrogen and phosphorus in sewage
A resource and sewage technology, applied in the field of nitrogen and phosphorus resource utilization in sewage, to achieve a virtuous cycle, improve absorption efficiency, and promote environmental protection.
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Embodiment 1
[0024] Embodiment 1 A method for rapidly utilizing nitrogen and phosphorus in sewage by anaerobic fermentation and duckweed cultivation
[0025] Sewage treatment process such as figure 1 , figure 2 shown. First, build a sedimentation well with a volume of 100 cubic meters and a brick-concrete structure. The sewage enters the No. 1 sedimentation well through the underground sewage pipe network. After 48 hours of sedimentation, the solid part of the lower layer is used as organic fertilizer, and the liquid part of the upper layer is added to the mass volume The ratio is 2% NaOH, add hydrochloric acid after standing for 36 hours to adjust the pH to 7.2, and enter the sewage anaerobic fermentation tank treatment system (UASB anaerobic reactor, 8 meters high, 3 meters in diameter, effective volume 56 cubic meters, disposable Treat 5 tons of sewage, purchased from Qingdao Yiweiyou Environmental Engineering Equipment Co., Ltd.), take anaerobic activated sludge as the inoculum, and...
Embodiment 2
[0026] Example 2 Duckweed Determination of Absorption Efficiency of Nitrogen and Phosphorus in Biogas Slurry
[0027] The biogas slurry obtained after treatment by the sewage anaerobic fermentation tank treatment system in Example 1 was used for the test of this example. A square plastic pot (length 20cm, width 15cm, height 10cm) was used as the test container, and 1000mL of biogas slurry was added, and then 2g fresh weight of the pre-cultivated Zihuaia spp. was added to each treatment for 15 days. There were 20 repetitions for each duckweed treatment, and 5 repetitions were set for the water sample without duckweed as the control. All treatments were cultured and observed in a laboratory with uniform natural light. The temperature in the laboratory was maintained at 25-30°C. During the test, 3 repetitions were randomly selected for the two duckweed treatments every 3 days, and 3 repetitions were taken for the control group, and the remaining repetitions were replaced with 1...
Embodiment 3
[0030] The mensuration of starch and protein content in the duckweed of embodiment 3 biogas slurry cultivation
[0031] The starch content was determined indirectly by the total sugar content method (starch content=total sugar content×0.909). The dried duckweed powder obtained in Example 1 was hydrolyzed by 1.2M hydrochloric acid in a boiling water bath. The pH was adjusted to 7.0 using 10M NaOH, and lead acetate was added to precipitate the protein. Use C18 solid-phase extraction column to filter supernatant, use HPLC (Thermo 2795, Thermo Corp., USA) to analyze hydrolysis reaction, detector uses evaporative light scattering detector (All-Tech ELSD 2000, All-tech., Corp., USA). For the detailed process, please refer to the literature Zhang, L., Zhao, H., Gan, M., Jin, Y., Gao, X., Chen, Q., Guan, J., Wang, Z., 2011.Application of simultaneous saccharification and fermentation (SSF) from viscosity reducing of raw sweet potato for bioethanol production at laboratory, pilot an...
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