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Bovine serum albumin surface molecular imprinted polymer and preparation method thereof

A bovine serum albumin and surface molecular imprinting technology, which is applied in the fields of chemistry and materials, can solve the problems of imprinting research difficulties, large volume, variability and inactivation, etc., and achieve the effect of good reusability, large adsorption capacity, and rapid adsorption

Inactive Publication Date: 2015-07-01
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is mainly because protein biomacromolecules have problems such as large size, complex structure, many binding sites, and easy denaturation and inactivation as templates for molecular imprinting. It is quite difficult to imprint them according to the traditional embedding method.

Method used

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  • Bovine serum albumin surface molecular imprinted polymer and preparation method thereof
  • Bovine serum albumin surface molecular imprinted polymer and preparation method thereof
  • Bovine serum albumin surface molecular imprinted polymer and preparation method thereof

Examples

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Effect test

Embodiment 1

[0035] Add 10 mg of γ-aminopropyltriethoxysilane-modified carbon nanotubes to 10 mL of pH7 phosphate buffer solution, place in a 100 mL Erlenmeyer flask for ultrasonic dispersion for 30 s, and magnetically stir at room temperature for 12 h to obtain the first solution. Dissolve 30mg of bovine serum albumin and 45mg of ionic liquid 1-propyl(trimethoxysilyl)-3-methylimidazole chloride in 10mL of pH7 phosphate buffer solution, and place in a refrigerator at 4°C for 12h to obtain the second solution . Dissolve 2.5 mL of tetraethoxysilane in 12.5 mL of pH7 phosphate buffer, mix the first solution with the second solution, stir magnetically at 4°C for 12 hours, then add tetraethoxysilane solution to the mixed solution, Magnetic stirring was continued at 4 °C for 48 h. After the reaction is completed, the solid and liquid phases are separated, and the solid phase product is washed four times with a phosphate buffer solution of pH 7, then eluted with ultrapure water until no protein ...

Embodiment 2

[0037]Add 10 mg of γ-aminopropyltriethoxysilane-modified carbon nanotubes to 10 mL of Tris-HCl buffer solution at pH 8, place in a 100 mL Erlenmeyer flask for ultrasonic dispersion for 30 s, and magnetically stir at room temperature for 12 h to obtain the first solution. Dissolve 30 mg of bovine serum albumin and 60 mg of ionic liquid 1-propyl (trimethoxysilyl)-3-methylimidazole chloride in 10 mL of Tris-HCl buffer solution at pH 8, and place in a refrigerator at 4°C for 12 hours to obtain the second parts solution. Dissolve 2.5 mL of tetraethoxysilane in 12.5 mL of Tris-HCl buffer solution with pH 8, mix the first solution with the second solution, stir magnetically at 4°C for 12 hours, then add tetraethoxysilane to the mixed solution The solution was continued to be magnetically stirred at 4°C for 48h. After the reaction is completed, the solid-liquid phases are separated, and the solid-phase product is washed four times with a Tris-HCl buffer solution with a pH of 8, and t...

Embodiment 3

[0039] Add 10 mg of γ-aminopropyltriethoxysilane-modified carbon nanotubes to 10 mL of Tris-HCl buffer solution with pH 8.9, place in a 100 mL Erlenmeyer flask for ultrasonic dispersion for 30 s, and magnetically stir at room temperature for 12 h to obtain the first solution . Dissolve 30 mg of bovine serum albumin and 80 mg of ionic liquid 1-propyl(trimethoxysilyl)-3-methylimidazole chloride in 10 mL of Tris-HCl buffer solution at pH 8.9, and place in a refrigerator at 4°C for 12 hours to obtain the first Two solutions. Dissolve 2.5 mL of tetraethoxysilane in 12.5 mL of Tris-HCl buffer solution with pH 8.9, mix the first solution with the second solution, stir magnetically at 4°C for 12 hours, and then add tetraethoxysilane to the mixed solution Base silane solution, continue to magnetically stir at 4°C for 48h. After the reaction is completed, the solid-liquid phases are separated, and the solid-phase product is washed four times with a Tris-HCl buffer solution with a pH o...

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Abstract

The invention belongs to the field of chemical and material and relates to a bovine serum albumin surface molecular imprinted polymer and a preparation method thereof. The method comprises the following steps: by taking a bovine serum albumin as a template molecule, taking r-aminopropyl triethoxysilane modified carbon nano-tube as a matrix and taking siloxane functional ion liquid as a functional monomer, under the effect of a cross-linking agent tetraethoxysilane, grafting a layer of molecular imprinted polymer with selective recognition ability for the bovine serum albumin on the surface of the carbon nano-tube. The molecular imprinted polymer prepared by the invention is good in thermal stability, is big in specific surface area, has an effective recognition site specifically combined with the bovine serum albumin, is large in bovine serum albumin absorption volume, is good in selectivity and specificity and is excellent in reusability.

Description

technical field [0001] The invention belongs to the field of chemistry and materials, and relates to a bovine serum albumin surface molecularly imprinted polymer and a preparation method thereof. Background technique [0002] Molecular imprinting technology is a technology that simulates antibody-antigen interaction and artificially prepares polymers that have specific binding capabilities for specific molecules (template molecules). Firstly, complementary interactions between template molecules and functional monomers form a template-monomer complex, and then under the action of a cross-linking agent, a bulk polymerization reaction occurs around the template-monomer complex to form a polymer. By removing the template molecule in the polymer under certain conditions, a selective molecular recognition material with a specific spatial structure cavity and a recognition site matching the template molecule can be obtained, which is commonly referred to as a molecularly imprinted...

Claims

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Application Information

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IPC IPC(8): C08G77/26C08G77/06C08K9/06C08K7/24B01J20/26B01J20/30
Inventor 刘名茗皮江燕林丹妮王小洁冯雄汉刘凡
Owner HUAZHONG AGRI UNIV
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