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Black rice anthocyanin extracting method

An extraction method, black rice flower technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of low stability, difficult product application, poor product quality, etc., and achieve easy control of the process and short heating time , fast heating effect

Inactive Publication Date: 2016-12-14
HENAN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The black rice anthocyanins obtained by common extraction methods currently contain more impurities such as sugars, organic acids, and proteins, which make the product poor in quality and low in stability, and cause great difficulties in product application.

Method used

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  • Black rice anthocyanin extracting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Extraction of Anthocyanins from Qindao No. 2 Black Rice

[0057] Use the grains of Qindao No. 2 black rice as raw material, dry at 35°C at low temperature, and then crush it into 60-mesh fine powder, accurately weigh 10.0g of the broken black rice fine powder, put it in a conical flask, add 100ml Ethanol / hydrochloric acid mixed solution, wherein the mixed solution is composed of 65% ethanol / 0.1mol / L acid whose V / V is 70 / 30, the solid-liquid ratio of black rice fine powder and mixed solution is 1:10, after the two are mixed evenly , the pH was adjusted to 3.8, and the anthocyanins were extracted from the black rice fine powder for 30 minutes at an extraction temperature of 50° C. and a power of 500 W under the action of ultrasound and microwave assistance.

[0058] Shake the mixture after ultrasonic and microwave-assisted extraction, centrifuge at 4000rpm / min for 15min, separate the filter residue and filtrate; take the filtrate and use vacuum rotary evaporation at 40°C,...

Embodiment 2

[0068] Extraction of Anthocyanins from Heixuan No.8

[0069] Use black rice grains of No. 8 black rice as raw material, dry at 35°C at low temperature, and then crush it into a fine powder of 40 meshes. Accurately weigh 10.0 g of the crushed black rice fine powder of No. 8 Xuan, and place it in a conical flask. Add the ethanol / acid mixed solution of 50ml, wherein mixed solution is to form by the hydrochloric acid of 60% ethanol / 0.05mol / L that V / V is 70 / 20, the solid-liquid ratio of black rice fine powder and mixed solution 1: 5. After mixing the two evenly, adjust the pH to 4.0, extract anthocyanins from No. 8 black rice fine powder for 50 minutes at an extraction temperature of 40°C and a power of 450W, with the help of ultrasonic waves and microwaves.

[0070] Shake the mixture extracted by ultrasonic and microwave assistance, centrifuge at 4000rpm / min for 10min, separate the filter residue and filtrate; take the filtrate and use vacuum rotary evaporation at 40°C, and concen...

Embodiment 3

[0079] Extraction of Anthocyanins from Heifeng Glutinous Black Rice

[0080] Use the grains of Heifeng glutinous black rice as raw materials, dry them at 35°C at low temperature, and then crush them into 20-mesh fine powder. Accurately weigh 10.0g of the crushed Heifeng glutinous black rice fine powder, place it in a conical flask, add The ethanol / acid mixed solution of 80ml, wherein mixed solution is made up of the hydrochloric acid of 85% ethanol / 0.2mol / L that V / V is 70 / 25, the solid-liquid ratio of Heifeng glutinous black rice fine powder and mixed solution is 1: 8, After the two were mixed evenly, the pH was adjusted to 3.8, and the anthocyanins were extracted from Heifenggluo black rice fine powder for 60 minutes at an extraction temperature of 45°C and a power of 500W, with the assistance of ultrasonic waves and microwaves.

[0081] Shake the mixture after ultrasonic and microwave-assisted extraction, centrifuge at 4000rpm / min for 15min, separate the filter residue and f...

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Abstract

The invention provides a black rice anthocyanin extracting method. The black rice anthocyanin extracting method specifically comprises the following steps: taking a black rice grain as a material, preprocessing the black rice grain, coarsely extracting the anthocyanin in the black rice grain, and then sequentially perform ultrafiltration, nanofiltration, macroporous resin adsorption, ethyl alcohol elution, secondary nanofiltration, and freeze-drying in vacuum to obtain a freeze-dried powder of the black rice anthocyanin. The black rice anthocyanin extracting method provided by the invention is good in selectivity; impurities of polysaccharide, organic acids, proteins and the like in the black rice anthocyanin can be effectively separated; the extracting rate is high, the product quality is high, even trace of the black rice anthocyanin is also extracted, the extracting speed is high, the operation is simple, and the discharge of effluent is low.

Description

technical field [0001] The invention relates to the technical field of extracting plant functional natural active ingredients, in particular to a method for extracting black rice anthocyanins. Background technique [0002] Anthocyanidin (Anthocyanidin) widely exists in the plant kingdom, is a water-soluble pigment, belongs to flavonoids in phenolic compounds. Anthocyanins are produced by the phenylpropionic acid pathway and the flavonoid biosynthetic pathway, which are catalyzed by the regulation of many enzymes. In the natural state, due to the instability of anthocyanins, they mainly exist in the plant cell fluid in the form of glycosides (called anthocyanins) in plants. Different anthocyanins show different colors, thus forming flowers, fruits, Stems and leaves come in a riot of beautiful colors including blue, purple, violet, magenta, red and orange. Anthocyanins have a strong antioxidant effect, can remove free radicals in the body; reduce fat content in serum and liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C09B67/54
CPCC09B61/00C09B67/0096
Inventor 马超贾琦石周爽庞玉辉冯雅岚李友军
Owner HENAN UNIV OF SCI & TECH
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