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Method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing guar gum

A mannose enzymatic hydrolysis solution and mannanase technology are applied in the biological field, which can solve the problems of long time, low enzymatic hydrolysis efficiency, low oligosaccharide, etc., and achieve production time and cost saving, high enzymatic hydrolysis speed, and improved enzymatic hydrolysis. The effect of efficiency

Active Publication Date: 2015-07-01
临沂小鲁生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One is that the existing method mainly adopts β-mannanase to the enzymatic hydrolysis of guar gum, but the efficiency of adopting a single enzyme to hydrolyze guar gum is not high
Although some existing technologies use the method of degrading plant polysaccharides with compound enzymes, since each enzyme degrades separately, it takes a long time and the enzymatic hydrolysis efficiency is low
The 2nd, in the guar gum enzymatic hydrolysis process, because the guar gum solution viscosity is too high, the concentration during the enzymolysis guar gum that generally adopts at present is 0.3-2%, the concentration of the oligosaccharide that obtains is lower, in further When preparing galactomannose powder, dehydration and drying treatment is required, which not only increases the cost of subsequent dehydration and drying of the product, galactomannose, but also seriously affects the efficiency of industrial production

Method used

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  • Method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing guar gum
  • Method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing guar gum
  • Method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing guar gum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Take 10g of guar gum powder, dilute to 100mL with 0.1mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.0; then heat and swell at 92°C for 50min; after cooling to 30°C, At the same time, 700 U of β-mannanase, 10000 U of xylanase and 20000 U of cellulase were added and mixed thoroughly.

[0026] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0027] (1) Raise the temperature from the cooled temperature to 35°C, and perform enzymatic hydrolysis for 1.5h;

[0028] (2) Raise the temperature to 48°C and perform enzymatic hydrolysis for 3 hours; increase the temperature by 1°C every 3 minutes during the heating process, and keep it for 10 minutes for every 1°C increase;

[0029] (3) Raise the temperature to 55° C. and perform enzymatic hydrolysis for 2 hours.

[0030] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mass percentage...

Embodiment 2

[0034] Take 11g of guar gum powder, dilute to 100mL with 0.1mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.3; then heat and swell at a temperature of 93.5°C for 55min; after cooling to 31°C, At the same time, add 990 U of β-mannanase, 16500 U of xylanase, and 27500 U of cellulase, and mix well.

[0035] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0036] (1) Raise the temperature from the cooled temperature to 38°C, and perform enzymatic hydrolysis for 2 hours;

[0037] (2) Raise the temperature to 50°C and perform enzymatic hydrolysis for 3.5 hours; during the heating process, increase the temperature by 1°C every 4 minutes, and keep it for 10 minutes for every 1°C increase;

[0038] (3) Raise the temperature to 55° C. and perform enzymatic hydrolysis for 2.5 hours.

[0039] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The m...

Embodiment 3

[0043] Take 12g of guar gum powder, dilute to 100mL with 0.1mol / mL citric acid-sodium citrate buffer solution, adjust the pH value to 6.6; then heat and swell at 95°C for 60min; after cooling to 33°C, At the same time, add β-mannanase 1080U, xylanase 24000U, cellulase 36000U, and mix thoroughly;

[0044] After adding the three enzymes, the temperature gradient enzymatic hydrolysis method is adopted, specifically:

[0045] (1) Raise the temperature from the cooled temperature to 40°C, and perform enzymatic hydrolysis for 2.5 hours;

[0046] (2) Raise the temperature to 53°C and perform enzymatic hydrolysis for 4 hours; during the heating process, increase the temperature by 2°C every 5 minutes, and keep it for 10 minutes for every 2°C increase;

[0047] (3) Raise the temperature to 55° C., and perform enzymatic hydrolysis for 3 hours.

[0048] After the enzymatic hydrolysis, the oligogalactomannose enzymatic hydrolysis solution is obtained. The mass percentages of galactoman...

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Abstract

The invention discloses a method for rapidly preparing galactomannan oligosaccharide enzymatic hydrolysate by utilizing guar gum. The method is characterized by firstly preparing a guar gum solution with a citric acid-sodium citrate buffer solution, then controlling the temperature and time to carry out heating swelling, after cooling, adding beta-mannanase, xylanase and cellulase at the same time, fully mixing the materials uniformly and controlling the enzymolysis temperature and time to carry out degradation. The method has the effects of achieving rapid enzymolysis of guar gum at high concentration, obtaining the galactomannan oligosaccharide enzymatic hydrolysate and saving the drying and purifying costs during subsequent production of galactomannan oligosaccharide.

Description

technical field [0001] The invention relates to a biological enzymatic hydrolysis method for rapidly preparing oligogalactomannose by using guar gum, and belongs to the field of biotechnology. Background technique [0002] Guar gum is a natural thickener processed from the endosperm of guar beans, an annual herbaceous leguminous crop. The main component is a plant polysaccharide: galactomannan. Guar gum is a white or yellowish free-flowing powder, soluble in cold or hot water, and forms a jelly-like substance when it meets water, which can thicken rapidly. The main chain of the galactomannan structure is mannan linked by β-(1-4) glycosidic bonds, and the branch chain is galactan linked by α-(1-6) glycosidic bonds. Galactomannose is an incomplete degradation product of galactomannan. It is a general term for oligosaccharides polymerized by 2-10 galactose and mannose molecules through glycosidic bonds. The molar ratio of galactose and mannose About 1:2. Galactomannose oligo...

Claims

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Application Information

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IPC IPC(8): C12P19/14
Inventor 刘云国刘艳华徐云峰马云刘凌霄
Owner 临沂小鲁生物科技有限公司
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