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Application of potentilla anserine polysaccharide in preparation of hypoglycemic drugs or health products

The technology of fern hemp polysaccharide and health care product is applied in the application field of fern hemp polysaccharide in the preparation of hypoglycemic drugs or health care products, and can solve the problems of not lowering blood sugar in normal mice, lowering blood sugar, and having no statistical significance.

Inactive Publication Date: 2015-07-15
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wu Huiping, Zhong Qiliang, Zhang Zhe et al. "Effect of Fernia extract on α-glucosidase activity" ("New Drugs and Clinical Pharmacology of Traditional Chinese Medicine" 2010,21(1):4-6) and Wu Huiping, Gao Meifeng , Dai Xiaoming et al.’s patent on “An α-Glucosidase Inhibitor——Bernaxa Extract and Its Extraction Method” mentioned that the water extract of Brackenia had a tendency to reduce the blood sugar of normal mice 1 hour after a meal. , but there is no statistical significance compared with the non-administered group (ZL 200710131473.5), that is, it has no effect on reducing blood sugar in normal mice

Method used

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  • Application of potentilla anserine polysaccharide in preparation of hypoglycemic drugs or health products
  • Application of potentilla anserine polysaccharide in preparation of hypoglycemic drugs or health products
  • Application of potentilla anserine polysaccharide in preparation of hypoglycemic drugs or health products

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Experimental program
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Effect test

Embodiment 1

[0024] The extraction of embodiment 1 ferns polysaccharide

[0025] Weigh the dried Junima, crush it, add about 8 times the amount of water, put it on the electric stove and cook for 1.5 hours, then filter the aqueous solution, repeat 3 times, combine the filtrates obtained from the 3 times, put them in an evaporating dish and evaporate and concentrate to 1: 1. Put the concentrated solution in a beaker, add 5 times the amount of 95% v / v ethanol, and let stand at low temperature overnight. Put the precipitate in a centrifuge tube and centrifuge (3000r / min, 10min), discard the supernatant, then add absolute ethanol, acetone, and ether to wash thoroughly, then centrifuge (3000r / min, 10min), discard the supernatant. The resulting precipitate was placed in a plate, and the gray powder obtained after the ether volatilized was Bracken polysaccharide, which was weighed. The yield was 2.66%.

Embodiment 2

[0026] Embodiment 2 Determination of the polysaccharide content of Bracken

[0027] The phenol-concentrated sulfuric acid method was used to determine the content of the polysaccharides in Bracken. Precisely draw 0.16, 0.20, 0.24, 0.28, 0.32, 0.36, 0.40mL of glucose standard solution, divide into 10mL volumetric flasks, add distilled water to dilute to the mark and mix well. Accurately pipette 2.0 mL into 10 mL test tubes with stoppers, and take another 2.0 mL of distilled water as a blank control. Add 1.0 mL of phenol solution to each tube, mix well, and quickly add concentrated H 2 SO 4 5.0mL, mixed and left for 5min, heated in a boiling water bath for 15min, taken out and cooled in cold water, measured absorbance at 490nm. The calculated regression equation is: y=0.042x-0.6836 R=0.9906.

[0028] Precisely weigh about 4mg of Juema polysaccharide in a 100ml volumetric flask, add water to dissolve and dilute to the scale, accurately draw 1.0ml and put it in a test tube, me...

Embodiment 3

[0029] The establishment of embodiment 3 diabetes mouse model

[0030] The experimental animals used were 110 male Kunming mice, weighing 17-23 g, provided by the Animal Experiment Center of Lanzhou University School of Medicine.

[0031] Take 110 clean-grade male Kunming mice weighing (20±3) g, 10 of which are the blank control group, and the remaining 100 are divided into cages, marked and weighed, and injected with 70 mg / kg of alloxan into the tail vein. Fasting blood glucose was measured after 3 days, and those with blood glucose value >11.1mmol / L were diabetic models.

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Abstract

The invention discloses a new application of potentilla anserine polysaccharide, and in particular relates to an application of the potentilla anserine polysaccharide in preparation of hypoglycemic drugs or health products. A research shows that the potentilla anserine polysaccharide can significantly reduce the blood sugar of a diabetic model mouse and has a therapeutic effect on the diabetic model mouse, and points out that the potentilla anserine polysaccharide has a significant blood sugar reducing effect, and can be applied to preparation of the hypoglycemic drugs or health products; the potentilla anserine polysaccharide can be applied to the preparation of medicaments for treating diabetes, and can also be applied to the preparation of health products for assistant treatment of diabetes.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to the application of juniper polysaccharides in the preparation of hypoglycemic drugs or health care products. Background technique [0002] Diabetes mellitus is a group of clinical syndromes mainly manifested by glucose metabolism disorders caused by genetic and environmental factors. Diabetes can be complicated by a variety of chronic complications, leading to organ dysfunction and failure, and even disability and death. With the development of society and the improvement of people's living standards, the incidence of diabetes in our country is also increasing year by year. Diabetes is a metabolic disorder syndrome characterized by hyperglycemia, and the number of diabetic patients in the world is increasing at a rate of 6% per year. It is projected that between 2010 and 2030, the total number of adults with the disease will increase by 69% in d...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P3/10A23L1/30C08B37/00
Inventor 闵光宁闵光涛王利军
Owner LANZHOU UNIVERSITY