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An enzyme-free ECL glucose sensor

A technology of glucose sensor and g-c3n4, which is applied in the field of enzyme-free ECL glucose sensor to achieve the effect of high selectivity, weakened hindering effect and sensitive detection

Inactive Publication Date: 2018-08-24
SOUTHWEST UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The sensitivity, selectivity, and stability of existing enzyme-free glucose sensors still need to be further improved

Method used

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  • An enzyme-free ECL glucose sensor
  • An enzyme-free ECL glucose sensor
  • An enzyme-free ECL glucose sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] 1. Preparation of g-C 3 N 4 Nanosheets

[0062] Take 20g of melamine powder in a ceramic crucible, cover it, heat up to 600°C at a heating rate of 3°C / min, calcinate for 2 hours, and cool naturally to room temperature to obtain a yellow block g-C 3 N 4 powder. Take 250mg block g-C 3 N 4 The powder was dispersed in 250mL water and ultrasonicated for 10h. Centrifuge the obtained suspension at a rate of 5000rpm for 5min to remove the unpeeled chunks of g-C 3 N 4 powder. The supernatant was dried to obtain g-C 3 N 4 Nanosheets.

[0063] 2. Preparation of sensors

[0064] Glassy carbon electrodes (GCE, Φ=4mm) were respectively treated with 0.3 and 0.05 μm Al 2 o 3 After powder polishing, it was cleaned ultrasonically in distilled water, ethanol and double distilled water, and left to dry at room temperature. 1mg g-C 3 N 4 Nanosheets and 1mg PTCA were dispersed in 2mL water and sonicated for 8 hours to obtain pink g-C 3 N 4 - Dispersions of PTCA nanocomposi...

Embodiment 2

[0106] 1. Preparation of g-C 3 N 4 Nanosheets

[0107] Take 20g of melamine powder in a ceramic crucible, cover it, heat up to 595°C at a heating rate of 2.5°C / min, calcinate for 2.5h, and cool naturally to room temperature to form a yellow block g-C 3 N 4 powder. Take 250mg block g-C 3 N 4 The powder was dispersed in 250mL water and ultrasonicated for 9.5h. Centrifuge the obtained suspension at a rate of 4900rpm for 8min to remove the unpeeled chunks of g-C 3 N 4 powder. The supernatant was dried to obtain g-C3 N 4 Nanosheets.

[0108] 2. Preparation of sensors

[0109] Glassy carbon electrodes (GCE, Φ=4mm) were respectively treated with 0.3 and 0.05 μm Al 2 o 3 After powder polishing, it was cleaned ultrasonically in distilled water, ethanol and double distilled water, and left to dry at room temperature. 1mg g-C 3 N 4 Nanosheets and 0.9mg PTCA were dispersed in 1.995mL water, sonicated for 7 hours to obtain pink g-C 3 N 4 - Dispersions of PTCA nanocomposit...

Embodiment 3

[0119] 1. Preparation of g-C 3 N 4 Nanosheets

[0120] Take 20g of melamine powder in a ceramic crucible, cover it, heat up to 605°C at a heating rate of 3.5°C / min, calcinate for 1.5h, and cool naturally to room temperature to form a yellow block g-C 3 N 4 powder. Take 250mg block g-C 3 N 4 The powder was dispersed in 250mL water and ultrasonicated for 10.5h. Centrifuge the obtained suspension at a rate of 5200rpm for 4min to remove the lumps of g-C that have not yet peeled off 3 N 4 powder. The supernatant was dried to obtain g-C 3 N 4 Nanosheets.

[0121] 2. Preparation of sensors

[0122] Glassy carbon electrodes (GCE, Φ=4mm) were respectively treated with 0.3 and 0.05 μm Al 2 o 3 After powder polishing, it was cleaned ultrasonically in distilled water, ethanol and double distilled water, and left to dry at room temperature. 1mg g-C 3 N 4 Nanosheets and 1.1mg PTCA were dispersed in 1.995mL water, sonicated for 9h to obtain pink g-C 3 N 4 - Dispersions of ...

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Abstract

The invention relates to the field of a glucose sensor, and in particular relates to a non-enzyme electrogenerated chemiluminescence (ECL) glucose sensor. The glucose sensor is mainly made of graphite-like carbon nitride, 3,4,9,10-perylenetetracarboxylic acid, benzene ring dextranum and concanavalin protein. Based on the specific binding ability of ConA and glucose and excellent ECL property of g-C3N4-PTCA nano composite, the non-enzyme ECL glucose sensor can detect glucose sensitively under physiological conditions, the structured non-enzyme ECL glucose sensor has higher selectivity, stability and reproducibility, and the detection is more sensitive.

Description

technical field [0001] The invention relates to the field of glucose sensors, in particular to an enzyme-free ECL glucose sensor. Background technique [0002] Glucose is the main source of energy for cell metabolism, but elevated blood sugar may increase the risk of diabetes, which can lead to kidney failure or heart disease. Therefore, glucose detection is increasingly valued in clinical as well as biochemical sampling and plays an increasingly important role in improving the quality of life. Common glucose detection methods include high performance liquid chromatography, spectrophotometry, polarimetry, gas chromatography and electrochemical methods. [0003] Since Clark and Lyons reported the first enzyme electrode in 1962, enzyme biosensors have been widely used in glucose detection because of their high sensitivity, fast response and low cost. However, it is well known that enzymes are sensitive to temperature, pH, toxic substances, and humidity. For example, glucose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 陈时洪谭兴容
Owner SOUTHWEST UNIV
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