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Detection method for compounded Sichuan fritillary bulb extract tablets

A technology of compound Chuanbei essence tablet and detection method, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of identification of unephedra root and Bai Wei, etc., and achieve equivalent extraction effect, stable baseline of the spectrum, and good separation Effect

Active Publication Date: 2015-07-15
SICHUAN FENGCHUN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the existing detection methods of Fufang Chuanbeijing Tablets and Capsules, neither Ephedra root nor Baiwei was identified

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0060] Preparation of reference substance solution: take 10 mg of ephedrine hydrochloride reference substance, accurately weigh, put in a 100ml measuring bottle, add methanol to dilute to the mark, shake up, accurately measure 2ml, put in a 25ml measuring bottle, add mobile phase to dilute to the mark , Shake well to make a reference solution containing 8 μg of ephedrine hydrochloride per 1 ml.

[0061] Preparation of the test solution: Take 20 tablets of this product, grind them finely, accurately weigh 0.4-0.6g of this product, put it in a 100ml measuring bottle, add 50-70ml of methanol, and ultrasonicate for 15-20min at a power of 120W and a frequency of 59KHz , add methanol to dilute to the mark, shake well, filter, and discard the initial filtrate to obtain the test solution.

[0062] Determination method: Accurately draw 8-12 μl each of the reference substance solution and the test solution, inject it into a liquid chromatograph, and measure; each tablet of this product ...

Embodiment 1

[0071] (1) traits

[0072] This product is a sugar-coated tablet, yellowish brown after removing the sugar coating; fragrant and bitter.

[0073] (2) Microscopic observation

[0074] Take this product and observe it under a microscope. Calcium oxalate needle crystals form bundles, 32-144 μm long, present in mucus cells or scattered; Character-like or horseshoe-shaped, layered pattern can be seen.

[0075] (3) TLC qualitative identification

[0076] TLC qualitative identification of ephedrine and ephedrine A: take 10 tablets of this product, grind finely, add 30ml of water to dissolve, shake and extract twice with ether, the amount of ether used each time is 20ml; discard the ether solution , the water liquid is evaporated to dryness in a water bath, the residue is added with ethanol 20ml to dissolve, filtered, the filtrate is evaporated to dryness in a water bath, and the residue is added to 1ml of methanol to dissolve, as the test solution; Add methyl alcohol and make eve...

Embodiment 2

[0086] (1) traits

[0087] This product is a sugar-coated tablet, yellowish brown after removing the sugar coating; fragrant and bitter.

[0088] (2) Microscopic observation

[0089] Take this product and observe it under a microscope. Calcium oxalate needle crystals form bundles, 32-144 μm long, present in mucus cells or scattered; Character-like or horseshoe-shaped, layered pattern can be seen.

[0090] (3) TLC qualitative identification

[0091] Qualitative identification of ephedrine and ephedrine A by TLC: take 10 tablets of this product, grind finely, add 40ml of water to dissolve, shake and extract with ether for 3 times, the amount of ether used each time is 30ml; discard the ether solution , the water liquid water bath evaporates to dryness, the residue adds ethanol 30ml and makes dissolving, filters, and the filtrate water bath evaporates to dryness, the residue adds methanol 2ml and makes dissolving, as need testing solution; Get ephedrine reference substance and...

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Abstract

The invention discloses a detection method for compounded Sichuan fritillary bulb extract tablets. The detection method comprises the steps of carrying out character detection, carrying out microscopic observation, carrying out thin-layer chromatography qualitative identification and carrying out content determination, wherein thin-layer chromatography qualitative identification comprises the steps of identifying ephedrannin and neoephedrine A in a preparation, identifying polygala root and cynanchum atratum and identifying glycyrrhiza uralensis; content determination comprises the step of determining the content of ephedrine hydrochloride in the preparation. The detection method disclosed by the invention has the advantages that the mixing of radix ephedrae and cynanchum atratum is effectively controlled, and meanwhile, the composition and content of the compounded Sichuan fritillary bulb extract tablets are effectively controlled, so that the drug can be safer and more effective.

Description

technical field [0001] The invention relates to a method for detecting medicines, in particular to a method for detecting compound Chuanbeijing tablets. Background technique [0002] Compound Chuanbei Jing Tablets is a variety recorded in the nineteenth volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health". The standard number is WS-B-3622-98. Mother, polygala (remove licorice root), tangerine peel, French pinellia, bellflower, licorice extract, are tablets made of pure traditional Chinese medicine. It has the functions of resolving phlegm and relieving cough, relieving lung and relieving asthma. It is clinically used to treat acute and chronic bronchitis, bronchiectasis, cough, phlegm and dyspnea caused by phlegm blocking the lung and lung failure. However, in the original standard, only the microscopic identification of Pinellia chinensis was carried out, and the identification of ephedra by TLC was carried out. There was n...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
Inventor 钟茂团吴诗惠何德中古莉
Owner SICHUAN FENGCHUN PHARMA
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