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Edible fungi culturing method

A cultivation method and technology for edible fungi, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as affecting the health of bacterial species, how to use disinfectants without public disclosure, and unfavorable bacterial growth, and achieve good killing. Effect, destructive power is not strong, the effect of broad sterilization spectrum

Active Publication Date: 2015-07-22
湖南绿洲植物资源开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through the above-mentioned method, the disinfectant for cultivating edible fungi of the present invention can eliminate and prevent the miscellaneous bacteria that may appear in the cultivation process of various edible fungus strains such as bacteria, actinomycetes, saccharomyces, mold; but , its invention is only a kind of disinfectant, and the prescription components are complicated. Its invention does not disclose how the disinfectant is used in the process of strain cultivation, and there is no specific operation plan and use effect. local disinfection of the species, it cannot disinfect the species itself and the culture medium, there is no essential difference from the disinfection with ethanol alone, it has no ability to deal with the species itself, there is no significance of preventing and treating the species in advance, and there is no change in the cultivation method of edible fungi and process that did not have a positive impact on
In addition to potassium permanganate, this invention also contains a large amount of other chemical substances. For edible fungus strains, the addition and use of anti-fungal agents, antibiotics or various chemical substances will affect the health of the strain itself, which is not conducive to the growth of bacteria. growth of species

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 The Cordyceps coccidioides bacterial classification collected in the field-the subsea bacterial classification

[0026] (1) Take 100g of shredded potatoes, 50g of rice grains, 100g of fresh corn grains, 50g of wheat bran, 10g of glucose, 2g of superphosphate, 2g of sodium propionate, add 500ml of water, mix well, heat and decoct, and stir to form a paste, Pack equal volumes into tissue culture bottles with a volume of 300ml, sterilize at 125°C for 35 minutes, let cool, take out, cover tightly, and cool to room temperature to obtain the culture medium;

[0027] (2) Get the Cordyceps coccidioides bacterial classification-the stroma bacterial classification collected from the field, soak in 0.8% potassium permanganate solution 10 times the strain volume under fully open conditions, and replace the high Potassium manganate solution, soaked for 120 minutes;

[0028] (3) Under fully open conditions, insert the strains treated in step (2) into the culture medium obt...

Embodiment 2

[0030] Embodiment 2 pollutes armillaria bacterial classification

[0031] (1) Take 100g of shredded potatoes, 50g of rice grains, 100g of fresh corn grains, 50g of wheat bran, 10g of glucose, 2g of superphosphate, 2g of sodium propionate, add 500ml of water, mix well, heat and decoct, and stir to form a paste, Pack equal volumes into tissue culture bottles with a volume of 300ml, sterilize at 120°C for 25 minutes, let cool, take out, cover tightly, and cool to room temperature to obtain the culture medium;

[0032] (2) Take the Armillaria strains that have been placed in the freezer for one year and have a small amount of green mold and black mold due to power outages. Soak in medium temperature, replace the potassium permanganate solution every 30 minutes, and soak for 60 minutes in total;

[0033] (3) Under fully open conditions, insert the strains treated in step (2) into the medium obtained in step (1), and cultivate them at 20°C for 5 days, most of the strain bottles and...

Embodiment 3

[0035] Embodiment 3 pollutes Poria cocos strain cultivar

[0036] Get the cultivar of the Poria cocos strain that has been cultivated for 10 days, and the bacterial classification has grown a small number of molds including green mold, black mold, and yellow mold; under fully open conditions, add 0.4% high A mixed solution of potassium manganate and 0.4% sodium propionate, soak the bacteria and culture medium together for 120 minutes, replace the mixed solution every 30 minutes, pour out the mixed solution after soaking, and continue to cultivate, most of the bacteria bottles and bags have no Pollution, complete success, a small number of bacteria bottles and bacteria bags are still polluted, the pollution rate is 30%.

[0037] The solution containing potassium permanganate and sodium propionate is ready-to-use.

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Abstract

The invention relates to an edible fungi culturing method. The method includes the following steps that under the fully-open condition, edible fungi cultures are continuously cultured after being soaked through a solution containing potassium permanganate with the concentration ranging from 0.3% to 0.8% for 60 minutes to 120 minutes. The method is easy and convenient to operate, excellent in effect and especially suitable for culturing of cultures collected in the field or polluted cultures or cultures with infectious microbes.

Description

technical field [0001] The invention relates to a method for cultivating edible fungi, in particular to a method for continuing to cultivate after soaking the edible fungus in a solution containing potassium permanganate. Background technique [0002] The isolation and cultivation of edible fungus strains is a very important research field, and millions of tons of edible fungi strains are needed every year. The separation and purification of wild strains and the preservation of cultivated strains, three-stage propagation, purification and rejuvenation all require the preparation of mother species, original species, and cultivated species. The traditional method of preparing mother species is inclined test tube species, 200-500 yuan / branch, the test tube mouth is made of cotton plugs, chemical fiber plugs, silicone plugs, etc., which are easy to contaminate, loosen, fall off, and the amount of bacteria is very small. , the test tube is too long to take and inoculate the st...

Claims

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Application Information

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IPC IPC(8): A01G1/04A01H4/00
Inventor 谢昭明黄丹何亚辉
Owner 湖南绿洲植物资源开发有限公司
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