A large-size hydrophilic organic polymer liquid phase monolithic chromatographic column and its preparation and application

A monolithic chromatographic column and hydrophilic organic technology, which is applied in the field of large-size hydrophilic organic polymer liquid-phase monolithic chromatographic column and its preparation and application, and can solve problems that affect the research and application of large-sized monolithic chromatographic columns, unsatisfactory separation effects, and overall Low column efficiency and other issues, to achieve excellent separation ability and separation efficiency, low cost, not easy to break and collapse

Active Publication Date: 2016-08-24
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a major problem in the above-mentioned studies: the column efficiency of the monolithic column is low, the mechanical strength is poor, and the separation effect is not ideal.
Obviously, these shortcomings have greatly affected the further research and application of large-scale monolithic chromatographic columns

Method used

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  • A large-size hydrophilic organic polymer liquid phase monolithic chromatographic column and its preparation and application
  • A large-size hydrophilic organic polymer liquid phase monolithic chromatographic column and its preparation and application
  • A large-size hydrophilic organic polymer liquid phase monolithic chromatographic column and its preparation and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Monomer 3-[N,N-dimethyl-[2-(2-methylprop-2-enoyloxy)aminopropyl]ammonium]propane-1-sulfonic acid inner salt 0.0870g, The joint agent N, N-methylenebisacrylamide 0.0132g, the porogen methanol 0.2100g and the initiator azobisisobutyronitrile 0.0020g are prepared into a reaction mixture, which is ultrasonically degassed for 5 minutes and poured into the pretreated Then, seal both ends of the quartz capillary and put it in a 60°C water bath for 12 hours; after the reaction, connect the quartz capillary to a high-pressure pump, wash away impurities and unreacted substances, and obtain a large-sized SPP-co-MBA (400 micron internal diameter) hydrophilic organic polymer liquid monolithic chromatographic column, the electron scanning electron microscope result of organic polymer in the column is as follows figure 1 and figure 2 shown.

Embodiment 2

[0037] Monomer 3-[N,N-dimethyl-[2-(2-methylprop-2-enoyloxy)aminopropyl]ammonium]propane-1-sulfonic acid inner salt 0.0851g, The coupling agent N, N-methylenebisacrylamide 0.0150g, the porogen methanol 0.2089g and the initiator azobisisobutyronitrile 0.0033g are prepared into a reaction mixture, and after ultrasonic degassing, it is poured into pretreated quartz In the capillary, then seal both ends of the quartz capillary and put it in a 60°C water bath to react for 12 hours. After the reaction is completed, connect the quartz capillary to a high-pressure pump, rinse to remove unreacted substances, and obtain a large-sized SPP-co-MBA (400 micron inner diameter ) Hydrophilic organic polymer liquid phase monolithic chromatographic column.

Embodiment 3

[0039] Monomer 3-[N,N-dimethyl-[2-(2-methylprop-2-enoyloxy)aminopropyl]ammonium]propane-1-sulfonic acid inner salt 0.0900g, The coupling agent N, N-methylenebisacrylamide 0.0104g, the porogen methanol 0.2119g and the initiator azobisisobutyronitrile 0.0035g are prepared into a reaction mixture, and after ultrasonic degassing, pour it into a pretreated quartz capillary Then seal both ends of the quartz capillary and put it in a 60°C water bath to react for 12 hours. After the reaction is completed, connect the quartz capillary to a high-pressure pump, rinse to remove unreacted substances, and obtain a large-sized SPP-co-MBA (400 micron inner diameter) Hydrophilic organic polymer liquid phase monolithic column.

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Abstract

The invention belongs to the technical field of chromatographic separation materials and discloses a large-size hydrophilic organic polymer liquid monolithic chromatographic column as well as preparation and an application. According to the chromatographic column, 3-[N,N-dimethyl-[2-(2-methylpropane-2-alkene acyloxy) aminopropyl] ammonio] propane-1-sulfonate is taken as a function monomer, N,N-methylene bisacrylamide is taken as a cross-linking agent, methanol is taken as a hole forming agent, azodiisobutyronitrile is taken as an initiator, and the chromatographic column is formed through in situ polymerization of the materials in a pretreated quartz capillary tube with an inner diameter larger than 200 mu m by the aid of a thermocatalytic reaction. The inner diameter of the chromatographic column is larger than that of an ordinary organic polymer monolithic chromatographic column, the sample loading amount is larger, and the required flow speed is measured in microliter, so that requirements for an instrument is lower by comparison with a nanoliter liquid phase; the column pressure of the large-size hydrophilic organic polymer liquid monolithic chromatographic column is low, and the monolithic chromatographic column is suitable for separating multiple polar compounds such as base samples, nucleoside samples, small peptide samples and other samples.

Description

technical field [0001] The invention belongs to the technical field of chromatographic separation materials, and in particular relates to a large-size hydrophilic organic polymer liquid-phase monolithic chromatographic column and its preparation and application. Background technique [0002] As we all know, liquid chromatography and its combined technology have become an extremely important part of separation and analysis science, and have a very wide range of applications in various fields such as environment, food safety, pharmacy, medicine and life sciences. Chromatographic column is the core medium for liquid chromatography to realize its separation and analysis function, so the development of chromatographic column directly affects the development of the entire liquid chromatography technology and even various separation and analysis science fields. In recent years, new monolithic materials for chromatographic stationary phases, which have the advantages of high permeab...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01D15/22B01D15/20B01J20/26B01J20/30B01J20/281C07K1/16G01N30/02G01N30/60
Inventor 江正瑾刘杵胜韩海张婷婷郭嘉亮李海斌
Owner JINAN UNIVERSITY
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